Human N-Cadherin Antibody MAB13881: R&D Systems

Human N-Cadherin Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects mouse N-Cadherin in direct ELISAs and Western blots. In direct ELISAs approximately 50% cross-reactivity with recombinant mouse N-Cadherin is observed, and no cross-reactivity with recombinant human (rh) E-Cadherin, rhP-Cadherin, rhVE-Cadherin, rhCadherin-4, -8, -11, -12, or -13 is observed.
  • Source
    Monoclonal Mouse IgM Clone # 691723
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse N-Cadherin
    Asp160-Ala724
    Accession # P19022.4
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • Immunohistochemistry
    8-25 µg/mL
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human, Mouse, and Rat N‑Cadherin by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line, C2C12 mouse myoblast cell line, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human N‑Cadherin Monoclonal Antibody (Catalog # MAB13881) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for N‑Cadherin at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of N-Cadherin in HeLa Human Cell Line by Flow Cytometry. HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human N‑Cadherin Monoclonal Antibody (Catalog # MAB13881, filled histogram) or isotype control antibody mouse IgM (open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgM Secondary Antibody (Catalog # F0116).
Immunohistochemistry
N‑Cadherin in Human Brain. N‑Cadherin was detected in immersion fixed paraffin-embedded sections of Alzheimer's human brain using Mouse Anti-Human N‑Cadherin Monoclonal Antibody (Catalog # MAB13881) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: N-Cadherin
Neuronal Cadherin (N-Cadherin or NCAD), also known as Cadherin-2 (CDH2), is a 130 kDa type I membrane protein belonging to the Cadherin superfamily of calcium-dependent adhesion molecules. Cadherins are involved in multiple processes including embryonic development, cell migration, and maintenance of epithelial integrity (1, 2). Human N‑Cadherin is synthesized with a 25 amino acid (aa) signal peptide and a 134 aa N‑terminal propeptide. The mature cell surface‑expressed protein consists of a 565 amino acid (aa) extracellular domain (ECD) that contains five Cadherin repeats, a 21 aa transmembrane segment, and a 161 aa cytoplasmic domain (3). Within the ECD, human N-Cadherin shares 98% aa sequence identity with mouse and rat N-Cadherin. In the nervous system, N-Cadherin mediates adhesion between the opposing faces of developing neuronal synapses and between Schwann cells and neuronal axons (4, 5). It interacts in cis or in trans homophilically and with the GluR2 subunit of neuronal AMPA receptors (1, 6). During synaptic maturation, its expression is lost from inhibitory terminals but maintained at excitatory terminals (5). ADAM10-mediated shedding of the N-Cadherin ECD alters cell-cell adhesion, synaptic development, and AMPA receptor activity (7, 8). N-Cadherin can also be cleaved at multiple additional sites within the intracellular or extracellular domains by Calpain, gamma ‑Secretase, and several MMPs (9 - 13). Cleavage of N‑Cadherin in atherosclerotic plaques contributes alternatively to vascular smooth muscle cell proliferation (MMP-9 and -12) or apoptosis (MMP‑7) (12, 13). Aberrant cell surface expression of the pro and mature forms of N-Cadherin in cancer results in increased tumor progression and invasiveness (14, 15). N-Cadherin also mediates the adhesion between hematopoeitic progenitor cells and mesenchymal stromal cells of the bone marrow (16).
  • References:
    1. Pokutta, S. and W.I. Weis (2007) Annu. Rev. Cell Dev. Biol. 23:237.
    2. Gumbiner, B.M. (2005) Nat. Rev. Mol. Cell Biol. 6:622.
    3. Reid, R.A. and J.J. Hemperly (1990) Nucleic Acids Res. 18:5896.
    4. Wanner, I.B. and P.M. Wood (2002) J. Neurosci. 22:4066.
    5. Benson, D.L. and H. Tanaka (1998) J. Neurosci. 18:6892.
    6. Saglietti, L. et al. (2007) Neuron 54:461.
    7. Reiss, K. et al. (2005) EMBO J. 24:742.
    8. Malinverno, M. et al. (2010) J. Neurosci. 30:16343.
    9. Jang, Y.-N. et al. (2009) J. Neurosci. 29:5974.
    10. Uemura, K. et al. (2006) Neurosci. Lett. 402:278.
    11. Hartland, S.N. et al. (2009) Liver Int. 29:966.
    12. Williams, H. et al. (2010) Cardiovasc. Res. 87:137.
    13. Dwivedi, A. et al. (2009) Cardiovasc. Res. 81:178.
    14. Maret, D. et al. (2010) Neoplasia 12:1066.
    15. Tanaka, H. et al. (2010) Nat. Med. 16:1414.
    16. Wein, F. et al. (2010) Stem Cell Res. 4:129.
  • Long Name:
    Neural Cadherin
  • Entrez Gene IDs:
    1000 (Human); 12558 (Mouse); 83501 (Rat)
  • Alternate Names:
    cadherin 2, N-cadherin (neuronal); cadherin 2, type 1, N-cadherin (neuronal); Cadherin-2; CD325 antigen; CD325; CDH2; CDHNcalcium-dependent adhesion protein, neuronal; CDw325; NCadherin; N-Cadherin; NCADN-cadherin 1; Neural cadherin; neural-cadherin
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Reelin is involved in transforming growth factor-β1-induced cell migration in esophageal carcinoma cells.
    Authors: Yuan Y, Chen H, Ma G, Cao X, Liu Z
    PLoS ONE, 2012;7(2):e31802.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
Cell and Tissue Staining Kits
Description Application Cat# Citations Images  

Anti-Mouse HRP-DAB Cell & Tissue Staining Kit

CTS002 14
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Immunohistochemistry Reagents
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Antigen Retrieval Reagent-Universal

CTS015 10
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Antigen Retrieval Reagent-Basic

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Mounting Medium

CTS011 4
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Antigen Retrieval Reagent Sampler (50 mL each)

CTS016
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Antigen Retrieval Reagent-Acidic

CTS014
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NorthernLights Guard Mounting Media

NL996
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VisUCyte Antigen Retrieval Reagent-Universal

VCTS023
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VisUCyte Antigen Retrieval Reagent-Acidic

VCTS022
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VisUCyte Antigen Retrieval Reagent-Basic

VCTS021
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Secondary Antibodies
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Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0102B 10  
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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

Flow, IHC, ICC NL007 9
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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse IgG Antibody

WB AF007 4
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Mouse IgM APC-conjugated Antibody

Flow F0117  
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Goat Anti-Mouse IgM PE-conjugated Antibody

Flow F0116  
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Goat Anti-Mouse IgM NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL020
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Goat Anti-Mouse IgM NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL019
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Goat Anti-Mouse IgM Fluorescein-conjugated Antibody

Flow F0118 1
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Goat Anti-Mouse IgM PerCP-conjugated Antibody

Flow F0119
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