Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all NCOA2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human NCOA2
Phe1090-Leu1247
Accession # Q15596

Specificity

Detects human NCOA2 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human (rh) NCOA1 is observed, and less than 1% cross-reactivity with rhNCOA4 and rhNCOA6 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human NCOA2 Antibody

Detection of Human NCOA2 antibody by Western Blot.

Detection of Human NCOA2 by Western Blot.

Western blot shows lysates of JAR human choriocarcinoma cell line, Hep3B human hepatocellular carcinoma cell line, and IMR-32 human neuroblastoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human NCOA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8010) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NCOA2 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human NCOA2 Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: JAR human choriocarcinoma cell line, Hep3B human hepatocellular carcinoma cell line, and IMR‑32 human neuroblastoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NCOA2

NCOA2 (Nuclear receptor COActivator 2; also TIF2 and GRIP-1) is a 150-160 kDa, ubiquitously expressed, nuclear member of the SRC/p160 nuclear receptor (NR) coactivator family of proteins. As noted, and although NCOA2 is a coactivator of hormone nuclear receptors, it also contributes to HIF-1 alpha :Arnt driven gene transcription. With respect to NRs, it not only has intrinsic activity that directly impacts hormone receptor gene activation, but also recruits additional coactivators to the NR:p160/NCOA2 complex, imparting additional complexity to target gene expression. NCOA2 binds directly to nuclear receptors via one or more LxxLL motifs that constitute an NID (NR Interaction Domain). It further uses its C-terminus to both recruit additional coactivators such as CBP and CARM1, and to modify key chromatin sites via an intrinsic HAT activity. Human NCOA2 is 1464 amino acids (aa) in length. It contains bHLH domain (aa 26-83), a PAS region (aa 119-183), four consecutive LxxLL motifs over aa 641-882, and a C-terminal LLxxLxxxL segment (aa 1079-1087). There are five utilized Ser/Thr phosphorylation sites, and three utilized acetylation sites on Lys. NCOA2 is known to undergo translocation and fuse with KAT6A/MOZ, creating a fusion product containing aa 870-1464 of NCOA2. Over aa 1090-1247, human NCOA2 shares 94% aa sequence identity with mouse NCOA2/GRIP-1.

Long Name

Nuclear Receptor Co-activator 2

Alternate Names

bHLHe75, HTIF2, KAT13C, TIF2

Entrez Gene IDs

10499 (Human); 17978 (Mouse); 83724 (Rat)

Gene Symbol

NCOA2

UniProt

Q15596 (Human)

Additional NCOA2 Products

Product Documents for Human NCOA2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human NCOA2 Antibody

For research use only

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