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Human Neuritin Antibody

Catalog #: MAB283 Datasheet / COA / SDS

Discontinued Product

MAB283 has been discontinued.
View all Neuritin products.
Detection of Neuritin in HepG2 Human Cell Line by Flow Cytometry.
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Human Neuritin Antibody Summary

Species Reactivity
Human
Specificity
Detects human Neuritin in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse Neuritin.
Source
Monoclonal Mouse IgG2B Clone # 79537
Purification
Protein A or G purified from ascites
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Neuritin
Ala28-Gly114
Accession # Q9NPD7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Neuritin. For natural samples, we recommend the use of Goat Anti-Human Neuritin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF283)
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of Neuritin antibody in HepG2 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Neuritin in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human Neuritin Monoclonal Antibody (Catalog # MAB283, filled histogram) or isotype control antibody (Catalog # MAB004, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neuritin

Neuritin is a 14 kDa GPI-linked molecule that is expressed on developing and differentiating neurons as well as on hepatocytes. Neuritin is upregulated in response to BDNF, NT-3, androgens, and hypoxia, and it promotes neurite extension and arborization. Mature human Neuritin shares 99% and 100% aa sequence identity with mouse and rat Neuritin, respectively.

Entrez Gene IDs
51299 (Human)
Alternate Names
dJ380B8.2; neuritin 1; Neuritin; NRN1; NRNMGC44811

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Product Datasheet
COA
SDS

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