Human NKG2A/CD159a PE-conjugated Antibody

(17 citations)   
  • Species Reactivity
    Human
  • Specificity
    Recognizes the human NKG2A/CD94 heterodimer. It does not recognize the NKG2C/CD94 heterodimer or the CD94 homodimer.
  • Source
    Monoclonal Mouse IgG2A Clone # 131411
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    BaF3 mouse pro-B cell line transfected with human NKG2A and CD94
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of NKG2A/CD159a in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human NCAM‑1/CD56 APC‑conjugated Monoclonal Antibody (Catalog # FAB2408A) and either (A) Mouse Anti-Human NKG2A/CD159a PE‑conjugated Monoclonal Antibody (Catalog # FAB1059P) or (B) Mouse IgG2A Phycoerythrin Isotype Control (Catalog # IC003P). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: NKG2A/CD159a

NKG2A is a type II transmembrane receptor having a single extracellular lectin-like domain and a cytoplasmic ITIM motif. It associates with CD94 and is expressed on NK cells and some activated T cell populations. The NKG2A/CD94 complex delivers an inhibitory signal upon recognition of its ligand, HLA-E.

  • Long Name:
    Natural Killer G2A
  • Entrez Gene IDs:
    3821 (Human); 16641 (Mouse)
  • Alternate Names:
    CD159 antigen-like family member A; CD159a antigen; CD159a; C-lectin type II protein; killer cell lectin-like receptor subfamily C, member 1; Klrc1; MGC13374; natural killer cell lectin; natural killer group protein 2; NK cell receptor A; NKG2; NKG2-1/B activating NK receptor; NKG2A; NKG2-A; NKG2-A/B type II integral membrane protein; NKG2-A/B-activating NK receptor; NKG2-A/NKG2-B type II integral membrane protein; NKG2AMGC59791; NKG2-B
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

17 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Activated hepatic stellate cells impair NK cell anti-fibrosis capacity through a TGF-?-dependent emperipolesis in HBV cirrhotic patients
    Authors: J Shi, J Zhao, X Zhang, Y Cheng, J Hu, Y Li, X Zhao, Q Shang, Y Sun, B Tu, L Shi, B Gao, FS Wang, Z Zhang
    Sci Rep, 2017;7(0):44544.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  2. Eomesodermin and T-bet mark developmentally distinct human natural killer cells
    Authors: A Collins, N Rothman, K Liu, SL Reiner
    JCI Insight, 2017;2(5):e90063.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. CD3(bright)CD56(+) T cells associate with pegylated interferon-alpha treatment nonresponse in chronic hepatitis B patients
    Sci Rep, 2016;6(0):25567.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. IL-21-Induced MHC Class II+ NK Cells Promote the Expansion of Human Uncommitted CD4+ Central Memory T Cells in a Macrophage Migration Inhibitory Factor-Dependent Manner
    Authors: Romain Loyon
    J Immunol, 2016;197(1):85-96.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Overexpression of CD85j in TNBC patients inhibits Cetuximab-mediated NK-cell ADCC but can be restored with CD85j functional blockade.
    Authors: Roberti M, Julia E, Rocca Y, Amat M, Bravo A, Loza J, Colo F, Loza C, Fabiano V, Maino M, Podhorzer A, Fainboim L, Barrio M, Mordoh J, Levy E
    Eur J Immunol, 2015;45(5):1560-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  6. Regulatory Dendritic Cells Restrain NK Cell IFN-gamma Production through Mechanisms Involving NKp46, IL-10, and MHC Class I-Specific Inhibitory Receptors.
    Authors: Spallanzani R, Torres N, Avila D, Ziblat A, Iraolagoitia X, Rossi L, Domaica C, Fuertes M, Rabinovich G, Zwirner N
    J Immunol, 2015;195(5):2141-8.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  7. Up-regulation of activating and inhibitory NKG2 receptors in allogeneic and autologous hematopoietic stem cell grafts.
    Authors: Picardi A, Mengarelli A, Marino M, Gallo E, Benevolo M, Pescarmona E, Cocco R, Fraioli R, Tremante E, Petti M, De Fabritiis P, Giacomini P
    J Exp Clin Cancer Res, 2015;34(0):98.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  8. NKP30-B7-H6 Interaction Aggravates Hepatocyte Damage through Up-Regulation of Interleukin-32 Expression in Hepatitis B Virus-Related Acute-On-Chronic Liver Failure.
    Authors: Zou Y, Bao J, Pan X, Lu Y, Liao S, Wang X, Wang G, Lin D
    PLoS ONE, 2015;10(8):e0134568.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  9. Human Induced Pluripotent Stem Cells Are Targets for Allogeneic and Autologous Natural Killer (NK) Cells and Killing Is Partly Mediated by the Activating NK Receptor DNAM-1.
    Authors: Kruse V, Hamann C, Monecke S, Cyganek L, Elsner L, Hubscher D, Walter L, Streckfuss-Bomeke K, Guan K, Dressel R
    PLoS ONE, 2015;10(5):e0125544.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  10. Long-term effect on natural killer cells by interferon-alpha therapy on the outcomes of HCV infection.
    Authors: Hu X, Jiang Y, Li X, Gao Y, Guo X, Chi X, Yan H, Feng J, Zhong J, Sun B, Shao X, Xiao W, Pan Y, Niu J
    J Interferon Cytokine Res, 2014;34(5):366-75.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  11. Intracellular poly(I:C) initiated gastric adenocarcinoma cell apoptosis and subsequently ameliorated NK cell functions.
    Authors: Qu, Jing, Hou, Zhaohua, Han, Qiuju, Jiang, Wen, Zhang, Cai, Tian, Zhigang, Zhang, Jian
    J Interferon Cytokine Res, 2014;34(1):52-9.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  12. Natural killer cells are characterized by the concomitantly increased interferon-gamma and cytotoxicity in acute resolved hepatitis B patients.
    Authors: Zhao J, Li Y, Jin L, Zhang S, Fan R, Sun Y, Zhou C, Shang Q, Li W, Zhang Z, Wang F
    PLoS ONE, 2012;7(11):e49135.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  13. A novel immunomodulatory mechanism of ribavirin in suppressing natural killer cell function.
    Authors: Ogbomo H, Michaelis M, Altenbrandt B, Doerr HW, Cinatl J
    Biochem. Pharmacol., 2010;79(2):188-97.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  14. Phenotype markers and cytokine intracellular production by CD8+ gammadelta T lymphocytes do not support a regulatory T profile in Behcet's disease patients and healthy controls.
    Authors: Clemente A, Cambra A, Munoz-Saa I, Crespi C, Pallares L, Juan A, Matamoros N, Julia MR
    Immunol. Lett., 2010;129(2):57-63.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  15. Binding of excreted and/or secreted products of adult hookworms to human NK cells in Necator americanus-infected individuals from Brazil.
    Authors: Teixeira-Carvalho A, Fujiwara RT, Stemmy EJ, Olive D, Damsker JM, Loukas A, Correa-Oliveira R, Constant SL, Bethony JM
    Infect. Immun., 2008;76(12):5810-6.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  16. Isolation and characterization of CD8+ regulatory T cells in multiple sclerosis.
    Authors: Correale J, Villa A
    J. Neuroimmunol., 2008;195(1):121-34.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  17. Functional impairment in circulating and intrahepatic NK cells and relative mechanism in hepatocellular carcinoma patients.
    Authors: Cai L, Zhang Z, Zhou L, Wang H, Fu J, Zhang S, Shi M, Zhang H, Yang Y, Wu H, Tien P, Wang FS
    Clin. Immunol., 2008;129(3):428-37.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
Expand to show all 17 Citations
Isotype Controls
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Mouse IgG2A PE-conjugated Antibody

Ctrl IC003P 13  
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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