Human NKG2A/CD159a Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1059G-100UG
Detection of NKG2A/CD159a in Human Blood Lymphocytes by Flow Cytometry.
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Human NKG2A/CD159a Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Recognizes the human NKG2A/CD94 heterodimer. It does not recognize the NKG2C/CD94 heterodimer or the CD94 homodimer.
Source
Monoclonal Mouse IgG2A Clone # 131411
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
BaF3 mouse pro-B cell line transfected with human NKG2A and CD94
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of NKG2A/CD159a in Human Blood Lymphocytes by Flow Cytometry. View Larger

Detection of NKG2A/CD159a in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human NKG2A/CD159a Alexa Fluor® 488-conjugated Mono­clonal Antibody (Catalog # FAB1059G) or (B) isotype control antibody (Catalog # IC003G) and Mouse Anti-Human NCAM‑1/CD56 PE‑conjugated Monoclonal Antibody (Catalog # FAB2408P). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: NKG2A/CD159a

NKG2A is a type II transmembrane receptor having a single extracellular lectin-like domain and a cytoplasmic ITIM motif. It associates with CD94 and is expressed on NK cells and some activated T cell populations. The NKG2A/CD94 complex delivers an inhibitory signal upon recognition of its ligand, HLA-E.

Long Name
Natural Killer G2A
Entrez Gene IDs
3821 (Human); 16641 (Mouse)
Alternate Names
CD159 antigen-like family member A; CD159a antigen; CD159a; C-lectin type II protein; killer cell lectin-like receptor subfamily C, member 1; Klrc1; MGC13374; natural killer cell lectin; natural killer group protein 2; NK cell receptor A; NKG2; NKG2-1/B activating NK receptor; NKG2A; NKG2-A; NKG2-A/B type II integral membrane protein; NKG2-A/B-activating NK receptor; NKG2-A/NKG2-B type II integral membrane protein; NKG2AMGC59791; NKG2-B

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