Human NKG2A/CD159a Alexa Fluor® 488-conjugated Antibody

FAB1059G 1 Citation Datasheet / COA / SDS

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FAB1059G-100UG

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Detection of NKG2A/CD159a in Human Blood Lymphocytes by Flow Cytometry.

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Human NKG2A/CD159a Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Recognizes the human NKG2A/CD94 heterodimer. It does not recognize the NKG2C/CD94 heterodimer or the CD94 homodimer.

Source

Monoclonal Mouse IgG_2A Clone # 131411

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

BaF3 mouse pro-B cell line transfected with human NKG2A and CD94

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25-1 µg/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry

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Detection of NKG2A/CD159a in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with (A) Mouse Anti-Human NKG2A/CD159a Alexa Fluor® 488-conjugated Mono-clonal Antibody (Catalog # FAB1059G) or (B) isotype control antibody (Catalog # IC003G) and Mouse Anti-Human NCAM-1/CD56 PE-conjugated Monoclonal Antibody (Catalog # FAB2408P). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: NKG2A/CD159a

NKG2A, also known as CD159a, is a type II transmembrane receptor having a single extracellular lectin-like domain and a cytoplasmic ITIM motif. It associates with CD94 and is expressed on NK cells and some activated T cell populations. The NKG2A/CD94 complex delivers an inhibitory signal upon recognition of its ligand, HLA-E.

Long Name

Natural Killer G2A

Entrez Gene IDs

3821 (Human); 16641 (Mouse); 102121106 (Cynomolgus Monkey)

Alternate Names

CD159 antigen-like family member A; CD159a antigen; CD159a; C-lectin type II protein; killer cell lectin-like receptor subfamily C, member 1; Klrc1; MGC13374; natural killer cell lectin; natural killer group protein 2; NK cell receptor A; NKG2; NKG2-1/B activating NK receptor; NKG2A; NKG2-A; NKG2-A/B type II integral membrane protein; NKG2-A/B-activating NK receptor; NKG2-A/NKG2-B type II integral membrane protein; NKG2AMGC59791; NKG2-B

Product Datasheets

Product Datasheet

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SDS

Related Research Areas

Citation for Human NKG2A/CD159a Alexa Fluor® 488-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Membrane bound IL-21 based NK cell feeder cells drive robust expansion and metabolic activation of NK cells
Authors: EO Ojo, AA Sharma, R Liu, S Moreton, MA Checkley-L, K Gupta, G Lee, DA Lee, F Otegbeye, RP Sekaly, M de Lima, DN Wald
Sci Rep, 2019;9(1):14916.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay