Human NKG2A/CD159a PerCP-conjugated Antibody

  (1 citations)
(1 Review)
    
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Product Details
Citations (1)
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  • Species Reactivity
    Human
  • Specificity
    Recognizes the human NKG2A/CD94 heterodimer. It does not recognize the NKG2C/CD94 heterodimer or the CD94 homodimer.
  • Source
    Monoclonal Mouse IgG2A Clone # 131411
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    BaF3 mouse pro-B cell line transfected with human NKG2A and CD94
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    PerCP (Peridinin-chlorophyll Protein Complex)
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of NKG2A/CD159a in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human NKG2A/CD159a PerCP‑conjugated Mono­clonal Antibody (Catalog # FAB1059C) and Mouse Anti-Human NCAM‑1/CD56 PE‑conjugated Mono­clonal Antibody (Catalog # FAB2408P). Quadrant markers were set based on isotype control antibody staining (Catalog # IC003C). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
      12 months from date of receipt, 2 to 8 degreesC as supplied.
Background: NKG2A/CD159a
NKG2A is a type II transmembrane receptor having a single extracellular lectin-like domain and a cytoplasmic ITIM motif. It associates with CD94 and is expressed on NK cells and some activated T cell populations. The NKG2A/CD94 complex delivers an inhibitory signal upon recognition of its ligand, HLA-E.
  • Long Name:
    Natural Killer G2A
  • Entrez Gene IDs:
    3821 (Human); 16641 (Mouse)
  • Alternate Names:
    CD159 antigen-like family member A; CD159a antigen; CD159a; C-lectin type II protein; killer cell lectin-like receptor subfamily C, member 1; Klrc1; MGC13374; natural killer cell lectin; natural killer group protein 2; NK cell receptor A; NKG2; NKG2-1/B activating NK receptor; NKG2A; NKG2-A; NKG2-A/B type II integral membrane protein; NKG2-A/B-activating NK receptor; NKG2-A/NKG2-B type II integral membrane protein; NKG2AMGC59791; NKG2-B
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Screening NK-, B- and T-cell phenotype and function in patients suffering from Chronic Fatigue Syndrome.
    Authors: Curriu M, Carrillo J, Massanella M, Rigau J, Alegre J, Puig J, Garcia-Quintana A, Castro-Marrero J, Negredo E, Clotet B, Cabrera C, Blanco J
    J Transl Med, 2013;11(0):68.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow

FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A PerCP-conjugated Isotype Control

Ctrl IC003C 1
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 10
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Flow Cytometry.

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 Flow Human Anonymous 06/11/2018
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Summary

Application Flow Cytometry
Sample Tested Peripheral blood mononuclear cells (PBMCs)
Species Human

Other Experimental Details

Other Experimental Details The purpose of this staining is to look at the expression level of NKG2A in NK among different HIV-1-infected patient cohorts. The PBMC was stained with NKG2A/CD159a PerCP together with CD56, CD16,Lin(CD3/CD14/CD19). the staining of NKG2A was good.

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