Detection of NKG2A/CD159a in Human Blood Lymphocytes by Flow Cytometry.
Human peripheral blood lymphocytes were stained with Mouse Anti-Human NKG2A/CD159a PerCP‑conjugated Monoclonal Antibody (Catalog # FAB1059C) and Mouse Anti-Human NCAM‑1/CD56 PE‑conjugated Monoclonal Antibody (Catalog # FAB2408P). Quadrant markers were set based on isotype control antibody staining (Catalog # IC003C). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 degreesC as supplied.
NKG2A is a type II transmembrane receptor having a single extracellular lectin-like domain and a cytoplasmic ITIM motif. It associates with CD94 and is expressed on NK cells and some activated T cell populations. The NKG2A/CD94 complex delivers an inhibitory signal upon recognition of its ligand, HLA-E.
Natural Killer G2A
Entrez Gene IDs:
3821 (Human); 16641 (Mouse)
CD159 antigen-like family member A; CD159a antigen; CD159a; C-lectin type II protein; killer cell lectin-like receptor subfamily C, member 1; Klrc1; MGC13374; natural killer cell lectin; natural killer group protein 2; NK cell receptor A; NKG2; NKG2-1/B activating NK receptor; NKG2A; NKG2-A; NKG2-A/B type II integral membrane protein; NKG2-A/B-activating NK receptor; NKG2-A/NKG2-B type II integral membrane protein; NKG2AMGC59791; NKG2-B
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Peripheral blood mononuclear cells (PBMCs)
Other Experimental Details
Other Experimental Details
The purpose of this staining is to look at the expression level of NKG2A in NK among different HIV-1-infected patient cohorts. The PBMC was stained with NKG2A/CD159a PerCP together with CD56, CD16,Lin(CD3/CD14/CD19). the staining of NKG2A was good.