Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Flow Cytometry, CyTOF-ready

Cited:

Neutralization, Flow Cytometry, Immunoprecipitation, Affinity Chromatography, Depletion, ELISA Capture, Functional Assay, CyTOF-reported

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 131411
View all NKG2A/CD159a Primary Antibodies »

Product Specifications

Immunogen

BaF3 mouse pro-B cell line transfected with human NKG2A and CD94

Specificity

Recognizes the human NKG2A/CD94 heterodimer. It does not recognize the NKG2C/CD94 heterodimer or the CD94 homodimer.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human NKG2A/CD159a Antibody

Detection of NKG2A/CD159a antibody in Human PBMCs antibody by Flow Cytometry.

Detection of NKG2A/CD159a in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A) and either (A) Mouse Anti-Human NKG2A/CD159a Monoclonal Antibody (Catalog # MAB1059) or (B) Mouse IgG2A Isotype Control (Catalog # MAB003) followed by Anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Detection of NKG2A/CD159a antibody in HEK293 Human Cell Line Transfected with Human NKG2A/CD159a and eGFP antibody by Flow Cytometry.

Detection of NKG2A/CD159a in HEK293 Human Cell Line Transfected with Human NKG2A/CD159a and eGFP by Flow Cytometry.

HEK293 Human embryonic kidney cell line transfected with either (A) Human NKG2A/CD159a, or (B) Human NKG2B/CD159b and eGFP was stained with Mouse Anti-Human NKG2A/CD159a Monoclonal Antibody (Catalog # MAB1059) followed by APC-conjugated anti-Mouse IgG secondary antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB003). View our protocol for Staining Membrane-associated Proteins.

Detection of NKG2A/CD159a by Flow Cytometry

Detection of NKG2A/CD159a by Flow Cytometry

Killer immunoglobulin-like receptor (KIR) expression is enriched in NKG2A- natural killer (NK) cells. (A) Day 12 expanded CD56bright NK cells were gated on NKG2A expression. (B) NKG2A+ (top) and NKG2A− (bottom) cells were interrogated for their expression of both KIR2DL1/DS1 and KIR3DL1/DS1 (left). KIR2DL2/DL3/DS2 expression was examined on the double positive cells (right). (C) Percent of total NKG2A+ or NKG2A− cells that are positive for all three KIRs. Statistical significance was assessed by Student’s t-test. (D) Day 0 CD56dim NK cells were gated on NKG2A expression. (E) NKG2A+ (top) and NKG2A− (bottom) cells were interrogated for their expression of both KIR2DL1/DS1 and KIR3DL1/DS1 (left). KIR2DL2/DL3/DS2 expression was examined on the double positive cells (right). (F) Percent of total NKG2A+ or NKG2A− cells that are positive for all three KIRs. Statistical significance was assessed by Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29456538), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of NKG2A/CD159a by Flow Cytometry

Detection of NKG2A/CD159a by Flow Cytometry

Killer immunoglobulin-like receptor (KIR) expression is enriched in NKG2A- natural killer (NK) cells. (A) Day 12 expanded CD56bright NK cells were gated on NKG2A expression. (B) NKG2A+ (top) and NKG2A− (bottom) cells were interrogated for their expression of both KIR2DL1/DS1 and KIR3DL1/DS1 (left). KIR2DL2/DL3/DS2 expression was examined on the double positive cells (right). (C) Percent of total NKG2A+ or NKG2A− cells that are positive for all three KIRs. Statistical significance was assessed by Student’s t-test. (D) Day 0 CD56dim NK cells were gated on NKG2A expression. (E) NKG2A+ (top) and NKG2A− (bottom) cells were interrogated for their expression of both KIR2DL1/DS1 and KIR3DL1/DS1 (left). KIR2DL2/DL3/DS2 expression was examined on the double positive cells (right). (F) Percent of total NKG2A+ or NKG2A− cells that are positive for all three KIRs. Statistical significance was assessed by Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/29456538), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human NKG2A/CD159a Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Human peripheral blood cells and HEK293 Human Cell Line Transfected with Human NKG2A/CD159a and eGFP

Flow Cytometry Panel Builder

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Advanced Features

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  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NKG2A/CD159a

NKG2A, also known as CD159a, is a type II transmembrane receptor having a single extracellular lectin-like domain and a cytoplasmic ITIM motif. It associates with CD94 and is expressed on NK cells and some activated T cell populations. The NKG2A/CD94 complex delivers an inhibitory signal upon recognition of its ligand, HLA-E.

Long Name

Natural Killer G2A

Alternate Names

CD159a, Klrc1, NKG2

Entrez Gene IDs

3821 (Human); 16641 (Mouse); 102121106 (Cynomolgus Monkey)

Gene Symbol

KLRC1

Additional NKG2A/CD159a Products

Product Documents for Human NKG2A/CD159a Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human NKG2A/CD159a Antibody

For research use only

Citations for Human NKG2A/CD159a Antibody

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