Human NKG2C/CD159c Antibody

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Detection of NKG2C/CD159c in Human Blood Lymphocytes by Flow Cytometry.
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Citations (18)
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Reviews (3)

Human NKG2C/CD159c Antibody Summary

Species Reactivity
Detects human NKG2C/CD159c. Detects human NKG2C/CD159c as part of the NKG2C/CD94 heterodimer in flow cytometry. No
cross-reactivity with the human NKG2A/CD94 heterodimer or with the human CD94 homodimer is detected.
Monoclonal Mouse IgG2B Clone # 134522
Protein A or G purified from hybridoma culture supernatant
BaF3 mouse pro-B cell line transfected with human NKG2C/CD159c and CD94
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Flow Cytometry
0.25 µg/106 cells
See below
Strauss-Albee, D.M. et al. (2015) Sci. Transl. Med. 7: 297ra115. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of NKG2C/CD159c antibody in Human Blood Lymphocytes antibody by Flow Cytometry. View Larger

Detection of NKG2C/CD159c in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A) and either (A) Mouse Anti-Human NKG2C/CD159c Monoclonal Antibody (Catalog # MAB1381) or (B) Mouse IgG2BFlow Cytometry Isotype Control (Catalog # MAB0041) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NKG2C/CD159c

Human NKG2C (NK cell Group 2 isoform C; Killer cell lectin-like receptor subfamily C, member 2) is a member of the C-type lectin-like superfamily of proteins. Natural killer (NK) receptors are expressed in both NK cells and cytotoxic CD8+ T cells and have both activating and inhibitory members (1-3). Regulation of the balance between the activating and inhibitory receptors is important and lack of such regulation has been implicated in autoimmunity (4). The NKG2 family includes seven receptors: NKG2A, -B, -C, -D, -E, -F, and -H, which is the longer isoform of NKG2E. Except for NKG2D and NKG2F, the NKG2 family members form heterodimers with CD94 (5, 6). NKG2C interacts with the adapter molecule DAP12 and acts as activating receptor when heterodimerized with CD94 (7). Human NKG2C is synthesized as a 231 amino acid (aa) protein that includes a 70 aa cytoplasmic domain, a 23 aa transmembrane segment, and a 138 aa extracellular domain (ECD). Within the ECD, human NKG2C shares 40% sequence identity with mouse NKG2C. NKG2C-CD94 heterodimers bind to the widely expressed nonclassical MHC-I molecule, HLA-E (Qa-1b in mouse), which presents a peptide derived from the signal peptide of classical MHC-I molecules (8, 9). Triggering the NKG2C-CD94 complex may activate the cytolytic activity and cytokine production of NK and CD8+ T cells (8, 10). Human cytomegalovirus (HCMV) infection promotes the differentiation and expansion of NKG2C+ NK cell subsets, possibly involving a cognate interaction of CD94/NKG2C with ligand(s) displayed by HCMV‑infected cells (11, 12). MAB1381 (clone 134522) displays potent agonistic activity and also blocks the binding of the NKG2C/CD94 heterodimer to HLA-E tetramers (13-15).

  1. Orbelyan, G.A. et al. (2014) J. Immunol. 193:610.
  2. Tassi I. et al. (2006) Immnunol Rev. 214:92.
  3. Lanier, L.L. (2008) Nat. Immunol. 9:495.
  4. Schleinitz, N. et al. (2010) Immunology. 174:2878.
  5. Lopez-Botet, M. et al. (2000) Hum. Immunol. 61:7.
  6. Braud, V.M. et al. (1998) Nature. 391:795.
  7. Lanier, L.L. (1998) Immunity 8:693.
  8. Vance, R.E. et al. (1999) J Exp Med 190:1801.
  9. Kaiser B.K. et al. (2005) J Immunol 174:2878.
  10. Bellón T. et al. (1999) J Immunol 162:3996.
  11. Pupuleku A. et al. (2017) Front Immunol. 8:1317.
  12. Hammer Q. et al. (2018) Nat Immunol. 19:453.
  13. Alici, E. et al. (2008) Blood 111:3155.
  14. Coupel, S. et al. (2007) Blood 109:2806.
  15. Fausther-Bovendo, H. et al. (2008) AIDS 22:217.
Long Name
Natural Killer G2C
Entrez Gene IDs
3822 (Human); 16642 (Mouse)
Alternate Names
CD159 antigen-like family member C; CD159c antigen; CD159c; killer cell lectin-like receptor subfamily C, member 2; KLRC2; MGC138244; NK cell receptor C; NKG2-C type II integral membrane protein; NKG2C; NKG2-C; NKG2CNKG2-C-activating NK receptor

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Citations for Human NKG2C/CD159c Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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  1. KLRC1 knockout overcomes HLA-E-mediated inhibition and improves NK cell antitumor activity against solid tumors
    Authors: Alice Mac Donald, Delphine Guipouy, William Lemieux, Mario Harvey, Louis-Jean Bordeleau, David Guay et al.
    Frontiers in Immunology
  2. Human Cytomegalovirus Antigen Presentation by HLA-DR+ NKG2C+ Adaptive NK Cells Specifically Activates Polyfunctional Effector Memory CD4+ T Lymphocytes
    Authors: Marcel Costa-García, Michelle Ataya, Manuela Moraru, Carlos Vilches, Miguel López-Botet, Aura Muntasell
    Frontiers in Immunology
  3. Depletion of central memory CD8+ T cells might impede the antitumor therapeutic effect of Mogamulizumab
    Authors: Y Maeda, H Wada, D Sugiyama, T Saito, T Irie, K Itahashi, K Minoura, S Suzuki, T Kojima, K Kakimi, J Nakajima, T Funakoshi, S Iida, M Oka, T Shimamura, T Doi, Y Doki, E Nakayama, R Ueda, H Nishikawa
    Nature Communications, 2021-12-14;12(1):7280.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. NKG2A/CD94 Is a New Immune Receptor for HLA-G and Distinguishes Amino Acid Differences in the HLA-G Heavy Chain
    Authors: GT Hò, AA Celik, T Huyton, W Hiemisch, R Blasczyk, GS Simper, C Bade-Doedi
    Int J Mol Sci, 2020-06-19;21(12):.
    Species: Human
    Sample Types: Cell Culture Supernates, Whole Cells
    Applications: Affinity Chromatography, ELISA Capture
  5. Between Innate and Adaptive Immune Responses: NKG2A, NKG2C, and CD8? T Cell Recognition of HLA-E Restricted Self-Peptides Acquired in the Absence of HLA-Ia
    Authors: WC Pump, T Kraemer, T Huyton, GT Hò, R Blasczyk, C Bade-Doedi
    Int J Mol Sci, 2019-03-22;20(6):.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Immunoprecipitation
  6. Microvesicles from malaria-infected red blood cells activate natural killer cells via MDA5 pathway
    Authors: W Ye, M Chew, J Hou, F Lai, SJ Leopold, HL Loo, A Ghose, AK Dutta, Q Chen, EE Ooi, NJ White, AM Dondorp, P Preiser, J Chen
    PLoS Pathog., 2018-10-04;14(10):e1007298.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Experimental Zika Virus Inoculation in a New World Monkey Model Reproduces Key Features of the Human Infection
    Authors: CY Chiu, C Sánchez-Sa, J Bouquet, T Li, S Yagi, M Tamhankar, VL Hodara, LM Parodi, S Somasekar, G Yu, LD Giavedoni, S Tardif, J Patterson
    Sci Rep, 2017-12-07;7(1):17126.
    Species: Marmoset
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. NFKB1 regulates human NK cell maturation and effector functions
    Authors: Vassilios Lougaris
    Clin. Immunol, 2016-12-03;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Adaptive NKG2C+ NK Cell Response and the Risk of Cytomegalovirus Infection in Kidney Transplant Recipients
    Authors: Dolores Redondo-Pa
    J. Immunol, 2016-12-02;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  10. NK Cell Proliferation Induced by IL-15 Transpresentation Is Negatively Regulated by Inhibitory Receptors.
    Authors: Anton O, Vielkind S, Peterson M, Tagaya Y, Long E
    J Immunol, 2015-10-09;195(10):4810-21.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Regulation of Adaptive NK Cells and CD8 T Cells by HLA-C Correlates with Allogeneic Hematopoietic Cell Transplantation and with Cytomegalovirus Reactivation.
    Authors: Horowitz A, Guethlein L, Nemat-Gorgani N, Norman P, Cooley S, Miller J, Parham P
    J Immunol, 2015-09-28;195(9):4524-36.
    Species: Human
    Sample Types: Whole Cells
    Applications: CyTof
  12. Antibody-mediated response of NKG2Cbright NK cells against human cytomegalovirus.
    Authors: Costa-Garcia M, Vera A, Moraru M, Vilches C, Lopez-Botet M, Muntasell A
    J Immunol, 2015-02-09;194(6):2715-24.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Polymorphism in human cytomegalovirus UL40 impacts on recognition of human leukocyte antigen-E (HLA-E) by natural killer cells.
    Authors: Heatley, Susan L, Pietra, Gabriell, Lin, Jie, Widjaja, Jacqueli, Harpur, Christop, Lester, Sue, Rossjohn, Jamie, Szer, Jeff, Schwarer, Anthony, Bradstock, Kenneth, Bardy, Peter G, Mingari, Maria Cr, Moretta, Lorenzo, Sullivan, Lucy C, Brooks, Andrew G
    J Biol Chem, 2013-01-18;288(12):8679-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. Autologous antitumor activity by NK cells expanded from myeloma patients using GMP-compliant components.
    Authors: Alici E, Sutlu T, Bjorkstrand B, Gilljam M, Stellan B, Nahi H, Quezada HC, Gahrton G, Ljunggren HG, Dilber MS
    Blood, 2008-01-11;111(6):3155-62.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  15. Expression and release of soluble HLA-E is an immunoregulatory feature of endothelial cell activation.
    Authors: Coupel S, Moreau A, Hamidou M, Horejsi V, Soulillou JP, Charreau B
    Blood, 2007-04-01;109(7):2806-14.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  16. New Insights into Lymphocyte Differentiation and Aging from Telomere Length and Telomerase Activity Measurements
    Authors: T Fali, L Papagno, C Bayard, Y Mouloud, J Boddaert, D Sauce, V Appay
    J. Immunol., 2019-02-08;0(0):.
  17. Irradiation-Induced Up-Regulation of HLA-E on Macrovascular Endothelial Cells Confers Protection against Killing by Activated Natural Killer Cells
    Authors: Isabelle Riederer, Wolfgang Sievert, Günther Eissner, Michael Molls, Gabriele Multhoff
    PLoS ONE
  18. Application of Mass Cytometry (CyTOF) for Functional and Phenotypic Analysis of Natural Killer Cells
    Authors: Alexander W. Kay, Dara M. Strauss-Albee, Catherine A. Blish
    Natural Killer Cells


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Human NKG2C/CD159c Antibody
By Anonymous on 09/23/2022
Application: Flow Sample Tested: B cell enriched peripheral blood mononuclear cells (PBMC) Species: Human

Human NKG2C/CD159c Antibody
By Anonymous on 09/23/2022
Application: qPCR Sample Tested: Peripheral blood mononuclear cells (PBMCs) Species: Human

Pre-incubation with antibody reduces stimulation through NKG2C

Human NKG2C/CD159c Antibody
By Anonymous on 09/23/2022
Application: WB Sample Tested: Peripheral blood mononuclear cells (PBMCs) Species: Human