Human NKG2C/CD159c Antibody

Name: Human NKG2C/CD159c Antibody
Brand: R&D Systems

(9 citations)

Datasheet / CoA / SDS

Flow Cytometry

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Product Details

Citations (9)

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Species Reactivity

Human
Specificity

Detects human NKG2C/CD159c. Detects human NKG2C/CD159c as part of the NKG2C/CD94 heterodimer in flow cytometry. No
cross-reactivity with the human NKG2A/CD94 heterodimer or with the human CD94 homodimer is detected.
Source

Monoclonal Mouse IgG_2B Clone # 134522
Purification

Protein A or G purified from hybridoma culture supernatant
Immunogen

BaF3 mouse pro-B cell line transfected with human NKG2C/CD159c and CD94
Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.
Label

Unconjugated

Product Datasheets

Product Datasheet

COA

Certificate of Analysis Lookup

Certificate of Analysis Request Form

SDS

Applications

Recommended
Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below
CyTOF-reported

Strauss-Albee, D.M. et al. (2015) Sci. Transl. Med. 7: 297ra115. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry

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Detection of NKG2C/CD159c in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human NCAM‑1/CD56 APC‑conjugated Monoclonal Antibody (Catalog # FAB2408A) and either (A) Mouse Anti-Human NKG2C/CD159c Monoclonal Antibody (Catalog # MAB1381) or (B) Mouse IgG_2B Flow Cytometry Isotype Control (Catalog # MAB0041) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NKG2C/CD159c

NKG2C, also known as hemoglobin scavenger receptor, is a type II transmembrane protein expressed exclusively in monocytes and macrophages. It is a scavenger receptor cysteine-rich superfamily (SRCR-SF) protein that contains nine SRCR motifs in its extracellular region. MAB1381 displays potent agonistic activity and also blocks the binding of the NKG2C/CD94 heterodimer to HLA-E tetramers (1-3).

References:
1. Alici, E. et al. (2008) Blood 111:3155.
2. Coupel, S. et al. (2007) Blood 109:2806.
3. Fausther-Bovendo, H. et al. (2008) AIDS 22:217.

Long Name:

Natural Killer G2C

Entrez Gene IDs:

3822 (Human); 16642 (Mouse)

Alternate Names:

CD159 antigen-like family member C; CD159c antigen; CD159c; killer cell lectin-like receptor subfamily C, member 2; KLRC2; MGC138244; NK cell receptor C; NKG2-C type II integral membrane protein; NKG2C; NKG2-C; NKG2CNKG2-C-activating NK receptor

Related Research Areas

Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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Species	Applications	Sample Type
Experimental Zika Virus Inoculation in a New World Monkey Model Reproduces Key Features of the Human Infection Authors: CY Chiu, C Sánchez-Sa, J Bouquet, T Li, S Yagi, M Tamhankar, VL Hodara, LM Parodi, S Somasekar, G Yu, LD Giavedoni, S Tardif, J Patterson Sci Rep, 2017;7(1):17126. Species: Marmoset Sample Type: Whole Cells Application: Flow Adaptive NKG2C+ NK Cell Response and the Risk of Cytomegalovirus Infection in Kidney Transplant Recipients Authors: Dolores Redondo-Pa J. Immunol, 2016;0(0):. Species: Human Sample Type: Whole Cells Application: Flow NFKB1 regulates human NK cell maturation and effector functions Authors: Vassilios Lougaris Clin. Immunol, 2016;0(0):. Species: Human Sample Type: Whole Cells Application: Flow Regulation of Adaptive NK Cells and CD8 T Cells by HLA-C Correlates with Allogeneic Hematopoietic Cell Transplantation and with Cytomegalovirus Reactivation. Authors: Horowitz A, Guethlein L, Nemat-Gorgani N, Norman P, Cooley S, Miller J, Parham P J Immunol, 2015;195(9):4524-36. Species: Human Sample Type: Whole Cells Application: CyTof Antibody-mediated response of NKG2Cbright NK cells against human cytomegalovirus. Authors: Costa-Garcia M, Vera A, Moraru M, Vilches C, Lopez-Botet M, Muntasell A J Immunol, 2015;194(6):2715-24. Species: Human Sample Type: Whole Cells Application: Flow NK Cell Proliferation Induced by IL-15 Transpresentation Is Negatively Regulated by Inhibitory Receptors. Authors: Anton O, Vielkind S, Peterson M, Tagaya Y, Long E J Immunol, 2015;195(10):4810-21. Species: Human Sample Type: Whole Cells Application: Flow Polymorphism in human cytomegalovirus UL40 impacts on recognition of human leukocyte antigen-E (HLA-E) by natural killer cells. Authors: Heatley, Susan L, Pietra, Gabriell, Lin, Jie, Widjaja, Jacqueli, Harpur, Christop, Lester, Sue, Rossjohn, Jamie, Szer, Jeff, Schwarer, Anthony, Bradstock, Kenneth, Bardy, Peter G, Mingari, Maria Cr, Moretta, Lorenzo, Sullivan, Lucy C, Brooks, Andrew G J Biol Chem, 2013;288(12):8679-90. Species: Human Sample Type: Whole Cells Application: Flow Autologous antitumor activity by NK cells expanded from myeloma patients using GMP-compliant components. Authors: Alici E, Sutlu T, Bjorkstrand B, Gilljam M, Stellan B, Nahi H, Quezada HC, Gahrton G, Ljunggren HG, Dilber MS Blood, 2008;111(6):3155-62. Species: Human Sample Type: Whole Cells Application: Neut Expression and release of soluble HLA-E is an immunoregulatory feature of endothelial cell activation. Authors: Coupel S, Moreau A, Hamidou M, Horejsi V, Soulillou JP, Charreau B Blood, 2007;109(7):2806-14. Species: Human Sample Type: Whole Cells Application: Neut

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Isotype Controls

Description Application Cat# Citations Images

Mouse IgG2B Isotype Control

Ctrl MAB004 34

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Human NKG2C/CD159c Antibody

Certificate of Analysis Lookup

Certificate of Analysis Lookup

Certificate of Analysis Request Form

FAQs

Mouse IgG2B Isotype Control

Mouse IgG2B Isotype Control

Mouse IgG2B Isotype Control

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