Human NKG2C/CD159c Antibody
Human NKG2C/CD159c Antibody Summary
cross-reactivity with the human NKG2A/CD94 heterodimer or with the human CD94 homodimer is detected.
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of NKG2C/CD159c in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A) and either (A) Mouse Anti-Human NKG2C/CD159c Monoclonal Antibody (Catalog # MAB1381) or (B) Mouse IgG2BFlow Cytometry Isotype Control (Catalog # MAB0041) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
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Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NKG2C/CD159c
Human NKG2C (NK cell Group 2 isoform C; Killer cell lectin-like receptor subfamily C, member 2) is a member of the C-type lectin-like superfamily of proteins. Natural killer (NK) receptors are expressed in both NK cells and cytotoxic CD8+ T cells and have both activating and inhibitory members (1-3). Regulation of the balance between the activating and inhibitory receptors is important and lack of such regulation has been implicated in autoimmunity (4). The NKG2 family includes seven receptors: NKG2A, -B, -C, -D, -E, -F, and -H, which is the longer isoform of NKG2E. Except for NKG2D and NKG2F, the NKG2 family members form heterodimers with CD94 (5, 6). NKG2C interacts with the adapter molecule DAP12 and acts as activating receptor when heterodimerized with CD94 (7). Human NKG2C is synthesized as a 231 amino acid (aa) protein that includes a 70 aa cytoplasmic domain, a 23 aa transmembrane segment, and a 138 aa extracellular domain (ECD). Within the ECD, human NKG2C shares 40% sequence identity with mouse NKG2C. NKG2C-CD94 heterodimers bind to the widely expressed nonclassical MHC-I molecule, HLA-E (Qa-1b in mouse), which presents a peptide derived from the signal peptide of classical MHC-I molecules (8, 9). Triggering the NKG2C-CD94 complex may activate the cytolytic activity and cytokine production of NK and CD8+ T cells (8, 10). Human cytomegalovirus (HCMV) infection promotes the differentiation and expansion of NKG2C+ NK cell subsets, possibly involving a cognate interaction of CD94/NKG2C with ligand(s) displayed by HCMV‑infected cells (11, 12). MAB1381 (clone 134522) displays potent agonistic activity and also blocks the binding of the NKG2C/CD94 heterodimer to HLA-E tetramers (13-15).
- Orbelyan, G.A. et al. (2014) J. Immunol. 193:610.
- Tassi I. et al. (2006) Immnunol Rev. 214:92.
- Lanier, L.L. (2008) Nat. Immunol. 9:495.
- Schleinitz, N. et al. (2010) Immunology. 174:2878.
- Lopez-Botet, M. et al. (2000) Hum. Immunol. 61:7.
- Braud, V.M. et al. (1998) Nature. 391:795.
- Lanier, L.L. (1998) Immunity 8:693.
- Vance, R.E. et al. (1999) J Exp Med 190:1801.
- Kaiser B.K. et al. (2005) J Immunol 174:2878.
- Bellón T. et al. (1999) J Immunol 162:3996.
- Pupuleku A. et al. (2017) Front Immunol. 8:1317.
- Hammer Q. et al. (2018) Nat Immunol. 19:453.
- Alici, E. et al. (2008) Blood 111:3155.
- Coupel, S. et al. (2007) Blood 109:2806.
- Fausther-Bovendo, H. et al. (2008) AIDS 22:217.
Product Datasheets
Citations for Human NKG2C/CD159c Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 10
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Depletion of central memory CD8+ T cells might impede the antitumor therapeutic effect of Mogamulizumab
Authors: Y Maeda, H Wada, D Sugiyama, T Saito, T Irie, K Itahashi, K Minoura, S Suzuki, T Kojima, K Kakimi, J Nakajima, T Funakoshi, S Iida, M Oka, T Shimamura, T Doi, Y Doki, E Nakayama, R Ueda, H Nishikawa
Nature Communications, 2021;12(1):7280.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
NKG2A/CD94 Is a New Immune Receptor for HLA-G and Distinguishes Amino Acid Differences in the HLA-G Heavy Chain
Authors: GT Hò, AA Celik, T Huyton, W Hiemisch, R Blasczyk, GS Simper, C Bade-Doedi
Int J Mol Sci, 2020;21(12):.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Affinity Chromatography -
Between Innate and Adaptive Immune Responses: NKG2A, NKG2C, and CD8? T Cell Recognition of HLA-E Restricted Self-Peptides Acquired in the Absence of HLA-Ia
Authors: WC Pump, T Kraemer, T Huyton, GT Hò, R Blasczyk, C Bade-Doedi
Int J Mol Sci, 2019;20(6):.
Species: Human
Sample Types: Recombinant Protein
Applications: Immunoprecipitation -
Microvesicles from malaria-infected red blood cells activate natural killer cells via MDA5 pathway
Authors: W Ye, M Chew, J Hou, F Lai, SJ Leopold, HL Loo, A Ghose, AK Dutta, Q Chen, EE Ooi, NJ White, AM Dondorp, P Preiser, J Chen
PLoS Pathog., 2018;14(10):e1007298.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Experimental Zika Virus Inoculation in a New World Monkey Model Reproduces Key Features of the Human Infection
Authors: CY Chiu, C Sánchez-Sa, J Bouquet, T Li, S Yagi, M Tamhankar, VL Hodara, LM Parodi, S Somasekar, G Yu, LD Giavedoni, S Tardif, J Patterson
Sci Rep, 2017;7(1):17126.
Species: Marmoset
Sample Types: Whole Cells
Applications: Flow Cytometry -
Adaptive NKG2C+ NK Cell Response and the Risk of Cytomegalovirus Infection in Kidney Transplant Recipients
Authors: Dolores Redondo-Pa
J. Immunol, 2016;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
NFKB1 regulates human NK cell maturation and effector functions
Authors: Vassilios Lougaris
Clin. Immunol, 2016;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Regulation of Adaptive NK Cells and CD8 T Cells by HLA-C Correlates with Allogeneic Hematopoietic Cell Transplantation and with Cytomegalovirus Reactivation.
Authors: Horowitz A, Guethlein L, Nemat-Gorgani N, Norman P, Cooley S, Miller J, Parham P
J Immunol, 2015;195(9):4524-36.
Species: Human
Sample Types: Whole Cells
Applications: CyTof -
NK Cell Proliferation Induced by IL-15 Transpresentation Is Negatively Regulated by Inhibitory Receptors.
Authors: Anton O, Vielkind S, Peterson M, Tagaya Y, Long E
J Immunol, 2015;195(10):4810-21.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Antibody-mediated response of NKG2Cbright NK cells against human cytomegalovirus.
Authors: Costa-Garcia M, Vera A, Moraru M, Vilches C, Lopez-Botet M, Muntasell A
J Immunol, 2015;194(6):2715-24.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Polymorphism in human cytomegalovirus UL40 impacts on recognition of human leukocyte antigen-E (HLA-E) by natural killer cells.
Authors: Heatley, Susan L, Pietra, Gabriell, Lin, Jie, Widjaja, Jacqueli, Harpur, Christop, Lester, Sue, Rossjohn, Jamie, Szer, Jeff, Schwarer, Anthony, Bradstock, Kenneth, Bardy, Peter G, Mingari, Maria Cr, Moretta, Lorenzo, Sullivan, Lucy C, Brooks, Andrew G
J Biol Chem, 2013;288(12):8679-90.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry -
Autologous antitumor activity by NK cells expanded from myeloma patients using GMP-compliant components.
Authors: Alici E, Sutlu T, Bjorkstrand B, Gilljam M, Stellan B, Nahi H, Quezada HC, Gahrton G, Ljunggren HG, Dilber MS
Blood, 2008;111(6):3155-62.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization -
Expression and release of soluble HLA-E is an immunoregulatory feature of endothelial cell activation.
Authors: Coupel S, Moreau A, Hamidou M, Horejsi V, Soulillou JP, Charreau B
Blood, 2007;109(7):2806-14.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
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Pre-incubation with antibody reduces stimulation through NKG2C
