Human NKG2C/CD159c Antibody

(6 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human NKG2C/CD159c. Detects human NKG2C/CD159c as part of the NKG2C/CD94 heterodimer in flow cytometry. No
    cross-reactivity with the human NKG2A/CD94 heterodimer or with the human CD94 homodimer is detected.
  • Source
    Monoclonal Mouse IgG2B Clone # 134522
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    BaF3 mouse pro-B cell line transfected with human NKG2C/CD159c and CD94
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • CyTOF-reported
    Strauss-Albee, D.M. et al. (2015) Sci. Transl. Med. 7: 297ra115. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of NKG2C/CD159c in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human NCAM‑1/CD56 APC‑conjugated Monoclonal Antibody (Catalog # FAB2408A) and either (A) Mouse Anti-Human NKG2C/CD159c Monoclonal Antibody (Catalog # MAB1381) or (B) Mouse IgG2B Flow Cytometry Isotype Control (Catalog # MAB0041) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NKG2C/CD159c
NKG2C, also known as hemoglobin scavenger receptor, is a type II transmembrane protein expressed exclusively in monocytes and macrophages. It is a scavenger receptor cysteine-rich superfamily (SRCR-SF) protein that contains nine SRCR motifs in its extracellular region. MAB1381 displays potent agonistic activity and also blocks the binding of the NKG2C/CD94 heterodimer to HLA-E tetramers (1-3).
  • References:
    1. Alici, E. et al. (2008) Blood 111:3155.
    2. Coupel, S. et al. (2007) Blood 109:2806.
    3. Fausther-Bovendo, H. et al. (2008) AIDS 22:217.
  • Long Name:
    Natural Killer G2C
  • Entrez Gene IDs:
    3822 (Human); 16642 (Mouse)
  • Alternate Names:
    CD159 antigen-like family member C; CD159c antigen; CD159c; killer cell lectin-like receptor subfamily C, member 2; KLRC2; MGC138244; NK cell receptor C; NKG2-C type II integral membrane protein; NKG2C; NKG2-C; NKG2CNKG2-C-activating NK receptor
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. Regulation of Adaptive NK Cells and CD8 T Cells by HLA-C Correlates with Allogeneic Hematopoietic Cell Transplantation and with Cytomegalovirus Reactivation.
    Authors: Horowitz A, Guethlein L, Nemat-Gorgani N, Norman P, Cooley S, Miller J, Parham P
    J Immunol, 2015;195(9):4524-36.
    Species: Human
    Sample Type: Whole Cells
    Application: CyTof
  2. Antibody-mediated response of NKG2Cbright NK cells against human cytomegalovirus.
    Authors: Costa-Garcia M, Vera A, Moraru M, Vilches C, Lopez-Botet M, Muntasell A
    J Immunol, 2015;194(6):2715-24.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  3. NK Cell Proliferation Induced by IL-15 Transpresentation Is Negatively Regulated by Inhibitory Receptors.
    Authors: Anton O, Vielkind S, Peterson M, Tagaya Y, Long E
    J Immunol, 2015;195(10):4810-21.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  4. Polymorphism in human cytomegalovirus UL40 impacts on recognition of human leukocyte antigen-E (HLA-E) by natural killer cells.
    Authors: Heatley, Susan L, Pietra, Gabriell, Lin, Jie, Widjaja, Jacqueli, Harpur, Christop, Lester, Sue, Rossjohn, Jamie, Szer, Jeff, Schwarer, Anthony, Bradstock, Kenneth, Bardy, Peter G, Mingari, Maria Cr, Moretta, Lorenzo, Sullivan, Lucy C, Brooks, Andrew G
    J Biol Chem, 2013;288(12):8679-90.
    Species: Human
    Sample Type: Whole Cells
    Application: Flow
  5. Autologous antitumor activity by NK cells expanded from myeloma patients using GMP-compliant components.
    Authors: Alici E, Sutlu T, Bjorkstrand B, Gilljam M, Stellan B, Nahi H, Quezada HC, Gahrton G, Ljunggren HG, Dilber MS
    Blood, 2008;111(6):3155-62.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
  6. Expression and release of soluble HLA-E is an immunoregulatory feature of endothelial cell activation.
    Authors: Coupel S, Moreau A, Hamidou M, Horejsi V, Soulillou JP, Charreau B
    Blood, 2007;109(7):2806-14.
    Species: Human
    Sample Type: Whole Cells
    Application: Neut
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