Human NMNAT-1 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human NMNAT‑1 in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    S. frugiperda insect ovarian cell line Sf 21-derived recombinant human NMNAT-1
    Ser4-Thr279
    Accession # Q9HAN9
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human NMNAT‑1 by Western Blot. Western blot shows lysates of human muscle tissue. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human NMNAT‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5865) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NMNAT‑1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NMNAT-1
NMNAT-1 is expressed in the nuclei of all human tissues, with highest expression in skeletal muscle, heart, kidney, pancreas, and brain (1). The enzyme transfers adenylate from ATP to nicotinamide ribonucleotide or nicotinate ribonucleotide to generate NAD+ or deamido-NAD+, and is an essential enzyme for the production of nuclear NAD+ (2). Nuclear NAD+ is required by poly(ADP-ribose) polymerase 1 (PARP-1), which poly-ADP-ribosylates chromatin in response to DNA strand breaks. NMNAT-1 is known to interact with PARP-1, resulting in its activation, but this interaction with PARP-1 is prevented when NMNAT-1 is phosphorylated at Ser136 (3). Nuclear NAD+ levels are also important for the regulation of SIR2 histone deacetylases (4). A naturally occurring Ube4b/NMNAT-1 chimeric protein is directly involved in slowing the degeneration of injured neurons in mice (5). NMNAT activity is required for the activation of tiazofurin, a drug used to treat leukemia (6). Two other NMNAT enzymes are present in humans. NMNAT-2 is localized in the Golgi complex and cytoplasm, and NMNAT-3 is a mitochondrial enzyme (7).
  • References:
    1. Emanuelli, M. et al. (2001) J. Biol.Chem. 276:406.
    2. Schweiger, M. et al. (2001) FEBS Lett. 492:95.
    3. Berger, F. et al. (2007) Proc. Natl. Acad. Sci. USA 104:3765.
    4. Revollo, J.R. et al. (2004) J. Biol. Chem. 279:50754.
    5. Mack, T.G. et al. (2001) Nature Neurosci. 4:1199.
    6. Boulton, S. et al. (1997) Br. J. Cancer 76:845.
    7. Berger, F. et al. (2005) J. Biol. Chem. 280:36334.
  • Long Name:
    Nicotinamide mononucleotide adenylyltransferase-1
  • Entrez Gene IDs:
    64802 (Human)
  • Alternate Names:
    EC 2.7.7.1; EC 2.7.7.18; NaMN adenylyltransferase 1; nicotinamide mononucleotide adenylyltransferase 1; nicotinamide nucleotide adenylyltransferase 1; nicotinamide nucleotide adenylyltransferase; Nicotinate-nucleotide adenylyltransferase 1; NMN adenylyltransferase 1; NMNAT1; NMNAT-1; NMNATPNAT1; PNAT1; pyridine nucleotide adenylyltransferase 1
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