Human NRG1‑ alpha /HRG1‑ alpha EGF Domain Antibody AF-296-NA: R&D Systems

Human NRG1‑ alpha /HRG1‑ alpha EGF Domain Antibody

(3 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human NRG1-alpha /HRG1-alpha in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human NRG1-alpha /HRG1-alpha
    Ser177-Lys241
    Accession # Q7RTV8
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Human NRG1-alpha /HRG1-alpha EGF Domain (Catalog # 296-HR)
  • Immunohistochemistry
    5-15 µg/mL
    See below
  • Neutralization
    Measured by its ability to neutralize NRG1‑ alpha /HRG1‑ alpha -induced proliferation in the MCF‑7 human breast cancer cell line. The Neutralization Dose (ND50) is typically 7-30 µg/mL in the presence of 125 ng/mL Recombinant Human NRG1‑ alpha /HRG1‑ alpha EGF Domain.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by NRG1‑ alpha /HRG1‑ alpha and Neutralization by Human NRG1‑ alpha /HRG1‑ alpha Antibody. Recombinant Human NRG1‑ alpha /HRG1‑ alpha EGF Domain (Catalog # 296-HR) stimulates proliferation in the MCF‑7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human NRG1‑ alpha /HRG1‑ alpha EGF Domain (125 ng/mL) is neutralized (green line) by increasing concentrations of Human NRG1‑ alpha /HRG1‑ alpha EGF Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-296-NA). The ND50 is typically 7-30 µg/mL.
Immunohistochemistry
NRG1-alpha /HRG1-alpha in Human Breast. NRG1-alpha /HRG1-alpha was detected in paraffin-embedded sections of human breast using Goat Anti-Human NRG1-alpha /HRG1-alpha EGF Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑296‑NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Neuregulin-1 alpha/NRG1 alpha

The neuregulin family of structurally related glycoproteins comprises products from four distinct but related genes, Nrg-1, Nrg-2, Nrg-3, and Nrg-4. Through alternative splicing or the use of alternative promoters, Nrg-1 encodes more than 14 soluble or transmembrane proteins. The extracellular domain of the transmembrane NRG1 isoforms can be proteolytically cleaved to release soluble growth factors. The alpha - or  beta -splice variants differ in their C-terminal region. All NRG1 isoforms contain an EGF-like domain that is required for their direct binding to the ErbB3 or ErbB4 receptor tyrosine kinases. The ErbB3 or ErbB4 subsequently recruits and heterodimerizes with ErbB2, resulting in tyrosine phosphorylation and NRG1 signaling. NRG1 isoforms can be classified into three major subtypes. Type I (Neu Differentiation Factor, NDF; Heregulin, HRG; Acetylcholine Receptor Inducing Activity, ARIA) and type II (Glial Growth Factor, GGF). NRG1s have an immunoglobulin (Ig)-like domain N‑terminal to the EGF-like domain. Type I NRG1s differ from type II NRG1s by having a glycosylation-rich domain between the Ig-like and the EGF-like domains. Type III NRG1 (Sensory and Motor neuron-Derived Factor) lacks the Ig-like domain but has a cysteine rich domain (CRD) instead. NRG1 isoforms show distinct spatial and temporal expression patterns. These proteins play important roles during development of both the nervous system and the heart. They have been shown to regulate the selective expression of neurotransmitter receptors in neurons and at the neuromuscular junction, and to promote the differentiation and development of Schwann cells from neural crest stem cells. NRG1s have also been shown to be involved in the establishment of the oligodendroglial lineage.

  • References:
    1. Lemke, G. (2006) Science STKE 325:pe11.
    2. Esper, R.M. et al. (2006) Brain Res. Brain Res. Rev. 51:161.
    3. Tosato, S. et al. (2005) Schizophr. Bull. 31:613.
  • Entrez Gene IDs:
    3084 (Human); 211323 (Mouse); 112400 (Rat)
  • Alternate Names:
    ARIA; GGF2; HGL; HRG; HRG1; MST131; MSTP131; neuregulin 1; Neuregulin1 alpha; Neuregulin-1 alpha; pro-neuregulin-1, membrane-bound isoform; pro-NRG1; sensory and motor neuron derived factor; SMDF
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Type I neuregulin1? is a novel local mediator to suppress hepatic gluconeogenesis in mice
    Authors: T Arai, Y Ono, Y Arimura, K Sayama, T Suzuki, S Shinjo, M Kanai, SI Abe, K Semba, N Goda
    Sci Rep, 2017;7(0):42959.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: WB
  2. Glial growth factor 2 promotes functional recovery with treatment initiated up to 7 days after permanent focal ischemic stroke.
    Authors: Iaci JF, Ganguly A, Finklestein SP, Parry TJ, Ren J, Saha S, Sietsma DK, Srinivas M, Vecchione AM, Caggiano AO
    Neuropharmacology, 2010;59(7):640-9.
    Species: Human
    Sample Type: Recombinant Protein
    Application: WB
  3. HER family receptor abnormalities in lung cancer brain metastases and corresponding primary tumors.
    Authors: Sun M, Behrens C, Feng L, Ozburn N, Tang X, Yin G, Komaki R, Varella-Garcia M, Hong WK, Aldape KD, Wistuba II
    Clin. Cancer Res., 2009;15(15):4829-37.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
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