|Cell Proliferation Induced by NRG1‑ alpha /HRG1‑ alpha and Neutralization by Human NRG1‑ alpha /HRG1‑ alpha Antibody. Recombinant Human NRG1‑ alpha /HRG1‑ alpha EGF Domain (Catalog # 296-HR) stimulates proliferation in the MCF‑7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human NRG1‑ alpha /HRG1‑ alpha EGF Domain (125 ng/mL) is neutralized (green line) by increasing concentrations of Human NRG1‑ alpha /HRG1‑ alpha EGF Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-296-NA). The ND50 is typically 7-30 µg/mL.|
|NRG1-alpha /HRG1-alpha in Human Breast. NRG1-alpha /HRG1-alpha was detected in paraffin-embedded sections of human breast using Goat Anti-Human NRG1-alpha /HRG1-alpha EGF Domain Antigen Affinity-purified Polyclonal Antibody (Catalog # AF‑296‑NA) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS009) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.|
The neuregulin family of structurally related glycoproteins comprises products from four distinct but related genes, Nrg-1, Nrg-2, Nrg-3, and Nrg-4. Through alternative splicing or the use of alternative promoters, Nrg-1 encodes more than 14 soluble or transmembrane proteins. The extracellular domain of the transmembrane NRG1 isoforms can be proteolytically cleaved to release soluble growth factors. The alpha - or beta -splice variants differ in their C-terminal region. All NRG1 isoforms contain an EGF-like domain that is required for their direct binding to the ErbB3 or ErbB4 receptor tyrosine kinases. The ErbB3 or ErbB4 subsequently recruits and heterodimerizes with ErbB2, resulting in tyrosine phosphorylation and NRG1 signaling. NRG1 isoforms can be classified into three major subtypes. Type I (Neu Differentiation Factor, NDF; Heregulin, HRG; Acetylcholine Receptor Inducing Activity, ARIA) and type II (Glial Growth Factor, GGF). NRG1s have an immunoglobulin (Ig)-like domain N‑terminal to the EGF-like domain. Type I NRG1s differ from type II NRG1s by having a glycosylation-rich domain between the Ig-like and the EGF-like domains. Type III NRG1 (Sensory and Motor neuron-Derived Factor) lacks the Ig-like domain but has a cysteine rich domain (CRD) instead. NRG1 isoforms show distinct spatial and temporal expression patterns. These proteins play important roles during development of both the nervous system and the heart. They have been shown to regulate the selective expression of neurotransmitter receptors in neurons and at the neuromuscular junction, and to promote the differentiation and development of Schwann cells from neural crest stem cells. NRG1s have also been shown to be involved in the establishment of the oligodendroglial lineage.
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