Detection of NTAL in THP‑1 Human Cell Line by Flow Cytometry.
THP‑1 human acute monocytic leukemia cell line was stained with Mouse Anti-Human NTAL APC‑conjugated Monoclonal Antibody (Catalog # IC4066A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). Cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Non-T cell activation linker (NTAL), also known as LAT2 and Linker for Activation of B cells (LAB), is a 26-28 kDa, 243 amino acid (aa) type III transmembrane (TM) adaptor protein involved in immunoreceptor signaling. NTAL belongs to the TRAP family of adaptors and is expressed in lipid raft microdomains of B cells, mast cells, neutrophils, monocytes, dendritic cells, activated T cells and NK cells. Rapid tyrosine phosphorylation of NTAL occurs upon BCR aggregation in B cells, Fc epsilon RI aggregation and Kit activation in mast cells, Fc gamma RI aggregation in monocytes, and Dectin-2 ligation on dendritric cells. Phosphorylated NTAL recruits signaling molecules such as Grb2, Gab1, and c-Cbl into receptor-signaling complexes. As a consequence, dendritic cells secrete IL-12, NK cells secrete IFN-gamma, B cells demonstrate an ability to present antigen, and mast cells show enhanced survival. Defects in the NTAL gene may cause Williams-Beuren syndrome, a rare genetic disorder characterized by mild mental retardation, and abnormalities in the cardiovascular and musculo-skeletal systems. One splice variant is known that shows a deletion of aa 112-243. Full-length human NTAL (aa 1-243) shares 49% aa sequence identity with aa 112-243 mouse NTAL.
Non-T Cell Activation Linker
Entrez Gene IDs:
7462 (Human); 56743 (Mouse); 317676 (Rat)
LAB; LABWilliams-Beuren syndrome chromosomal region 15 protein; Linker for activation of B-cells; linker for activation of T cells family, member 2; linker for activation of T-cells family member 2; Membrane-associated adapter molecule; Non-T-cell activation linker; NTAL; NTALWilliams-Beuren syndrome chromosomal region 5 protein; WBS15; WBSCR15; WBSCR5; WBSCR5HSPC046; Williams-Beuren syndrome chromosome region 5; WSCR5
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.