Human NUB1 Antibody

Catalog # Availability Size / Price Qty
AF7336
AF7336-SP
Detection of Human NUB1 by Western Blot.
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Human NUB1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human NUB1 in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human NUB1
Arg513-Asn615
Accession # Q9Y5A7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human NUB1 by Western Blot. View Larger

Detection of Human NUB1 by Western Blot. Western blot shows lysates of 786‑O human renal cell adenocarcinoma cell line, HeLa human cervical epithelial carcinoma cell line, K562 human chronic myelogenous leukemia cell line, Raji human Burkitt's lymphoma cell line, and human testis tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human NUB1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7336) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NUB1 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NUB1

NUB1 (NEDD8 Ultimate Buster-1; also NY-REN18) is a 68-74 kDa, TNF-alpha - and IFN-inducible protein that is found intracellularly in a wide variety of cells. It is reported to be a down-regulator of the NEDD8 conjugation system. NEDD8 is a ubiquitin-like protein that links to cullin family of proteins. Cullin family proteins are associated with large SCF/E3 ligase complexes. In the case of cullin-1, the activities of its SCF-associated complex include the ubiquitination of I kappa B alpha, beta -catenin and p21. Ubiquitination does not occur, however, unless cullin-1 is conjugated to NEDD8, making NEDD8 a key regulator of ubiquitination. NUB1 downregulates ubiquitination by binding, and targeting, both free and conjugated NEDD8 to the proteosome. Human NUB1 is 615 amino acids (aa) in length. It contains two coiled-coil regions (aa 36-70 and 152-203) and three UBA domains (aa 374-413, 424-470 and 489-529). There are three potential isoform variants. One possesses an alternative start site 24 aa upstream of the standard site, a second shows a deletion of aa 452-465, and a third shows a combination of the first two variants. Over aa 513-615, human NUB1 shares 84% aa sequence identity with mouse NUB1.

Long Name
NEDD8 Ultimate Buster 1
Entrez Gene IDs
51667 (Human); 53312 (Mouse); 296731 (Rat)
Alternate Names
BS4; NEDD8 ultimate buster-1; negative regulator of ubiquitin-like proteins 1NUB1L; NUB1; NUB1L; NY-REN-18 antigen; NY-REN-18; NYREN18NEDD8 ultimate buster 1; Renal carcinoma antigen NY-REN-18

Product Datasheets

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