Human Olfactomedin-2/Noelin-2 Antibody

Catalog # Availability Size / Price Qty
AF5614
AF5614-SP
Detection of Human Olfactomedin‑2/Noelin‑2 by Western Blot.
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Human Olfactomedin-2/Noelin-2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Olfactomedin‑2/Noelin‑2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant mouse (rm) Olfactomedin‑1 is observed and less than 5% cross-reactivity with recombinant human Olfactomedin‑4 and rmOlfactomedin‑L3 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Olfactomedin‑2/Noelin‑2
Gln21-Pro454
Accession # O95897
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Olfactomedin‑2/Noelin‑2 by Western Blot. View Larger

Detection of Human Olfactomedin‑2/Noelin‑2 by Western Blot.

Western blot shows lysates of human cortex and hippocampus tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Olfactomedin‑2/Noelin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5614) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Olfactomedin‑2/
Noelin‑2 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Olfactomedin-2/Noelin-2

Olfactomedin-2 (OLFM2, OM2), also called OlfC or neuronal olfactomedin-related endoplasmic reticulum-localized-2 (Noelin-2) is a 50 kDa (predicted), secreted member of the olfactomedin/noelin family of proteins (1‑3). Since Noelin-2 has also been used to designate an alternately spliced form of Noelin-1, Olfactomedin-2 is the less ambiguous name (4). Olfactomedin domain-containing proteins are often found in neural tissues (3). Human Olfactomedin-2 expressed sequence tags have mainly been found in the brain and eye, and RNA in the cerebellum (1, 2). In zebrafish, Olfactomedin-2 expression is developmentally regulated and, like Noelin-1, may play a role in neural crest cell differentiation (5). The human Olfactomedin-2 cDNA encodes a 20 amino acid (aa) signal sequence and a 454 mature protein that contains two coiled-coil regions (aa 58‑85 and 136‑193) and an Olfactomedin domain (aa 194‑446). Mature human Olfactomedin-2 shares 98% aa identity with rat, bovine and equine, 97% with mouse and canine, 81% with Xenopus, and 77% with zebrafish Olfactomedin-2. No alternate splice variants have been reported for Olfactomedin-2 (1). Polymorphisms of human Olfactomedin-2, with or without additional polymorphisms in optineurin (OPTN), have been associated with a small fraction of high intraocular pressure or open-angle glaucoma cases in Japanese patients (6). The family member myocilin shows stronger association with these ocular disorders, and either myocilin or Noelin-1 might heterodimerize with Olfactomedin-2 (1). Evidence suggests that Olfactomedin-2 has evolved from Noelin-1 gene duplication, and myocilin from Olfactomedin-2 gene duplication (1).

References
  1. Mukhopadhyay, A. et al. (2004) Mol. Vis. 10:304.
  2. Kulkarni, N.H. et al. (2000) Genet. Res. Camb. 76:41.
  3. Tomarev, S.I. and N. Nakaya (2009) Mol. Neurobiol. 40:122.
  4. Moreno, T.A. & M. Bronner-Fraser (2002) Mech. Dev. 119:121.
  5. Lee, J-A. et al. (2008) Mech. Dev. 125:167.
  6. Funayama, T. et al. (2006) Invest. Ophthalmol. Vis. Sci. 47:5368.
Entrez Gene IDs
93145 (Human); 244723 (Mouse); 313783 (Rat)
Alternate Names
neuronal olfactomedin related ER localized protein 2; NOE2; NOE2noelin 2; NOELIN2; Noelin-2; NOELIN2_V1; olfactomedin 2; Olfactomedin2; Olfactomedin-2; OlfC; OLFM2; OM2

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