Detects human Olfactomedin‑2/Noelin‑2 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross‑reactivity with recombinant mouse (rm) Olfactomedin‑1 is observed and less than 5% cross-reactivity with recombinant human Olfactomedin‑4 and rmOlfactomedin‑L3 is observed.
Polyclonal Sheep IgG
Chinese hamster ovary cell line CHO-derived recombinant human Olfactomedin‑2/Noelin‑2 Gln21-Pro454 Accession # O95897
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Olfactomedin‑2/Noelin‑2 by Western Blot.
Western blot shows lysates of human cortex and hippocampus tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Olfactomedin‑2/Noelin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5614) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Olfactomedin‑2/ Noelin‑2 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Olfactomedin-2 (OLFM2, OM2), also called OlfC or neuronal olfactomedin-related endoplasmic reticulum-localized-2 (Noelin-2) is a 50 kDa (predicted), secreted member of the olfactomedin/noelin family of proteins (1‑3). Since Noelin-2 has also been used to designate an alternately spliced form of Noelin-1, Olfactomedin-2 is the less ambiguous name (4). Olfactomedin domain-containing proteins are often found in neural tissues (3). Human Olfactomedin-2 expressed sequence tags have mainly been found in the brain and eye, and RNA in the cerebellum (1, 2). In zebrafish, Olfactomedin-2 expression is developmentally regulated and, like Noelin-1, may play a role in neural crest cell differentiation (5). The human Olfactomedin-2 cDNA encodes a 20 amino acid (aa) signal sequence and a 454 mature protein that contains two coiled-coil regions (aa 58‑85 and 136‑193) and an Olfactomedin domain (aa 194‑446). Mature human Olfactomedin-2 shares 98% aa identity with rat, bovine and equine, 97% with mouse and canine, 81% with Xenopus, and 77% with zebrafish Olfactomedin-2. No alternate splice variants have been reported for Olfactomedin-2 (1). Polymorphisms of human Olfactomedin-2, with or without additional polymorphisms in optineurin (OPTN), have been associated with a small fraction of high intraocular pressure or open-angle glaucoma cases in Japanese patients (6). The family member myocilin shows stronger association with these ocular disorders, and either myocilin or Noelin-1 might heterodimerize with Olfactomedin-2 (1). Evidence suggests that Olfactomedin-2 has evolved from Noelin-1 gene duplication, and myocilin from Olfactomedin-2 gene duplication (1).
Mukhopadhyay, A. et al. (2004) Mol. Vis. 10:304.
Kulkarni, N.H. et al. (2000) Genet. Res. Camb. 76:41.
Tomarev, S.I. and N. Nakaya (2009) Mol. Neurobiol. 40:122.
Moreno, T.A. & M. Bronner-Fraser (2002) Mech. Dev. 119:121.
Lee, J-A. et al. (2008) Mech. Dev. 125:167.
Funayama, T. et al. (2006) Invest. Ophthalmol. Vis. Sci. 47:5368.
Entrez Gene IDs:
93145 (Human); 244723 (Mouse); 313783 (Rat)
neuronal olfactomedin related ER localized protein 2; NOE2; NOE2noelin 2; NOELIN2; Noelin-2; NOELIN2_V1; olfactomedin 2; Olfactomedin2; Olfactomedin-2; OlfC; OLFM2; OM2
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