Human Olfactomedin-2/Noelin-2 Antibody Summary
Accession # O95897
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Olfactomedin‑2/Noelin‑2 by Western Blot.
Western blot shows lysates of human cortex and hippocampus tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Olfactomedin‑2/Noelin‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5614) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Olfactomedin‑2/
Noelin‑2 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Olfactomedin-2 (OLFM2, OM2), also called OlfC or neuronal olfactomedin-related endoplasmic reticulum-localized-2 (Noelin-2) is a 50 kDa (predicted), secreted member of the olfactomedin/noelin family of proteins (1‑3). Since Noelin-2 has also been used to designate an alternately spliced form of Noelin-1, Olfactomedin-2 is the less ambiguous name (4). Olfactomedin domain-containing proteins are often found in neural tissues (3). Human Olfactomedin-2 expressed sequence tags have mainly been found in the brain and eye, and RNA in the cerebellum (1, 2). In zebrafish, Olfactomedin-2 expression is developmentally regulated and, like Noelin-1, may play a role in neural crest cell differentiation (5). The human Olfactomedin-2 cDNA encodes a 20 amino acid (aa) signal sequence and a 454 mature protein that contains two coiled-coil regions (aa 58‑85 and 136‑193) and an Olfactomedin domain (aa 194‑446). Mature human Olfactomedin-2 shares 98% aa identity with rat, bovine and equine, 97% with mouse and canine, 81% with Xenopus, and 77% with zebrafish Olfactomedin-2. No alternate splice variants have been reported for Olfactomedin-2 (1). Polymorphisms of human Olfactomedin-2, with or without additional polymorphisms in optineurin (OPTN), have been associated with a small fraction of high intraocular pressure or open-angle glaucoma cases in Japanese patients (6). The family member myocilin shows stronger association with these ocular disorders, and either myocilin or Noelin-1 might heterodimerize with Olfactomedin-2 (1). Evidence suggests that Olfactomedin-2 has evolved from Noelin-1 gene duplication, and myocilin from Olfactomedin-2 gene duplication (1).
- Mukhopadhyay, A. et al. (2004) Mol. Vis. 10:304.
- Kulkarni, N.H. et al. (2000) Genet. Res. Camb. 76:41.
- Tomarev, S.I. and N. Nakaya (2009) Mol. Neurobiol. 40:122.
- Moreno, T.A. & M. Bronner-Fraser (2002) Mech. Dev. 119:121.
- Lee, J-A. et al. (2008) Mech. Dev. 125:167.
- Funayama, T. et al. (2006) Invest. Ophthalmol. Vis. Sci. 47:5368.
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