Human Osteocalcin Quantikine ELISA Kit

R&D Systems | Catalog # DSTCN0

Product is in the process of discontinuation. Please consider catalog # DY1419-05 as an alternative.
R&D Systems
Discontinued Product
DSTCN0 has been discontinued. View all Osteocalcin products.

Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), EDTA Plasma (50 µL)

Sensitivity

0.898 ng/mL

Assay Range

2-64 ng/mL (Cell Culture Supernates, EDTA Plasma)
View all Osteocalcin ELISA Kits »

Product Summary for Human Osteocalcin Quantikine ELISA Kit

The Quantikine Human Osteocalcin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Osteocalcin in cell culture supernates and EDTA plasma. It contains synthetic human Osteocalcin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Osteocalcin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Osteocalcin.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human Osteocalcin.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 4 4 4 4 4 4
Mean (ng/mL) 8.15 17.2 33.3 9.06 19.6 40.0
Standard Deviation 0.268 0.395 0.544 0.306 0.379 0.653
CV% 3.3 2.3 1.6 3.4 1.9 1.6

EDTA Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 4 4 4 4 4 4
Mean (ng/mL) 9.06 19.6 40.0 7.91 16.1 33.6
Standard Deviation 0.306 0.379 0.653 0.766 0.991 1.66
CV% 3.4 1.9 1.6 9.7 6.2 4.9

Recovery for Human Osteocalcin Quantikine ELISA Kit

The recovery of human Osteocalcin spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 105 98-120
EDTA Plasma (n=4) 102 80-116

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of human Osteocalcin were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human Osteocalcin ELISA Linearity

Scientific Data Images for Human Osteocalcin Quantikine ELISA Kit

Human Osteocalcin ELISA Cell Culture Supernate Standard Curve

Human Osteocalcin ELISA Cell Culture Supernate Standard Curve

Human Osteocalcin ELISA EDTA Plasma Standard Curve

Human Osteocalcin ELISA EDTA Plasma Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Osteocalcin

Osteocalcin, also named Bone gamma-carboxyglutamic Acid Protein, is a secreted protein whose expression is restricted to cells of the osteoblast lineage. It has been frequently used as a marker for osteoblast lineage cells.

Long Name

Bone gamma-Carboxyglutamate [gla] Protein

Alternate Names

BGLAP, BGP, OCN

Entrez Gene IDs

632 (Human); 12096 (Mouse); 25295 (Rat)

Gene Symbol

BGLAP

Additional Osteocalcin Products

Product Documents for Human Osteocalcin Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Osteocalcin Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human Osteocalcin Quantikine ELISA Kit

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  • Human Osteocalcin Quantikine ELISA Kit
    Name: Abby Sukarto
    Sample Tested: Human tissue
    Verified Customer | Posted 05/03/2017

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Protocols

View specific protocols for Human Osteocalcin Quantikine ELISA Kit (DSTCN0):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Human Osteocalcin Quantikine ELISA Kit

Showing  1 - 3 of 3 FAQs Showing All

  • Q: Are either of R&D Systems Human Osteocalcin ELISA assays, catalog #s DSTCN0 or DY1419, specific for un-carboxylated or gamma-carboxylated form of Osteocalcin?

    A: The Human Osteocaclin Quantikine ELISA (catalog # DSTCN0) assay recognizes natural and recombinant human Osteocalcin, and the assay recognizes Des-γ-carboxylated-Osteocalcin. The Human Osteocalcin DuoSet ELISA (catalog # DY419) also recognizes natural and recombinant human Osteocalcin, however, we have not determined if the DuoSet also recognizes Des-γ-carboxylated-Osteocalcin.

  • Q: Why can't heparin plasma samples be used with the Human Osteocalcin Quantikine ELISA Kit, Catalog # DSTCN0? 

    A: It was found that heparin tends to accelerate the enzymatic breakdown of osteocalcin and it is therefore not recommended with this kit. 

  • Q: Why is serum not a validated sample type with the Human Osteocalcin Quantikine ELISA Kit, Catalog # DSTCN0?

    A: Serum values have shown to be consistently lower than plasma samples in this kit and therefore are not an ideal sample type. 

  • Q: Are either of R&D Systems Human Osteocalcin ELISA assays, catalog #s DSTCN0 or DY1419, specific for un-carboxylated or gamma-carboxylated form of Osteocalcin?

    A: The Human Osteocaclin Quantikine ELISA (catalog # DSTCN0) assay recognizes natural and recombinant human Osteocalcin, and the assay recognizes Des-γ-carboxylated-Osteocalcin. The Human Osteocalcin DuoSet ELISA (catalog # DY419) also recognizes natural and recombinant human Osteocalcin, however, we have not determined if the DuoSet also recognizes Des-γ-carboxylated-Osteocalcin.

  • Q: Why can't heparin plasma samples be used with the Human Osteocalcin Quantikine ELISA Kit, Catalog # DSTCN0? 

    A: It was found that heparin tends to accelerate the enzymatic breakdown of osteocalcin and it is therefore not recommended with this kit. 

  • Q: Why is serum not a validated sample type with the Human Osteocalcin Quantikine ELISA Kit, Catalog # DSTCN0?

    A: Serum values have shown to be consistently lower than plasma samples in this kit and therefore are not an ideal sample type. 

  • Q: Are either of R&D Systems Human Osteocalcin ELISA assays, catalog #s DSTCN0 or DY1419, specific for un-carboxylated or gamma-carboxylated form of Osteocalcin?

    A: The Human Osteocaclin Quantikine ELISA (catalog # DSTCN0) assay recognizes natural and recombinant human Osteocalcin, and the assay recognizes Des-γ-carboxylated-Osteocalcin. The Human Osteocalcin DuoSet ELISA (catalog # DY419) also recognizes natural and recombinant human Osteocalcin, however, we have not determined if the DuoSet also recognizes Des-γ-carboxylated-Osteocalcin.

  • Q: Why can't heparin plasma samples be used with the Human Osteocalcin Quantikine ELISA Kit, Catalog # DSTCN0? 

    A: It was found that heparin tends to accelerate the enzymatic breakdown of osteocalcin and it is therefore not recommended with this kit. 

  • Q: Why is serum not a validated sample type with the Human Osteocalcin Quantikine ELISA Kit, Catalog # DSTCN0?

    A: Serum values have shown to be consistently lower than plasma samples in this kit and therefore are not an ideal sample type. 

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