Human Osteocalcin Quantikine ELISA Kit
Human Osteocalcin Quantikine ELISA Kit Summary
Product Summary
Precision
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 4 | 4 | 4 | 4 | 4 | 4 |
| Mean | 8.15 | 17.2 | 33.3 | 9.06 | 19.6 | 40 |
| Standard Deviation | 0.268 | 0.395 | 0.544 | 0.306 | 0.379 | 0.653 |
| CV% | 3.3 | 2.3 | 1.6 | 3.4 | 1.9 | 1.6 |
EDTA Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 4 | 4 | 4 | 4 | 4 | 4 |
| Mean | 9.06 | 19.6 | 40 | 7.91 | 16.1 | 33.6 |
| Standard Deviation | 0.306 | 0.379 | 0.653 | 0.766 | 0.991 | 1.66 |
| CV% | 3.4 | 1.9 | 1.6 | 9.7 | 6.2 | 4.9 |
Recovery
The recovery of human Osteocalcin spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 105 | 98-120 |
| EDTA Plasma (n=4) | 102 | 80-116 |
Linearity
Data Examples
Product Datasheets
Preparation and Storage
Background: Osteocalcin
Osteocalcin, also named Bone gamma-carboxyglutamic Acid Protein, is a secreted protein whose expression is restricted to cells of the osteoblast lineage. It has been frequently used as a marker for osteoblast lineage cells.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations for Human Osteocalcin Quantikine ELISA Kit
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
9
Citations: Showing 1 - 9
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Transcriptome Analysis of Mesenchymal Stem Cells from Multiple Myeloma Patients Reveals Downregulation of Genes Involved in Cell Cycle Progression, Immune Response, and Bone Metabolism
Authors: RC Fernando, DR Mazzotti, H Azevedo, AF Sandes, EG Rizzatti, MB de Oliveir, VLF Alves, AIP Eugênio, F de Carvalh, MA Dalboni, DC Martins, GWB Colleoni
Sci Rep, 2019;9(1):1056.
Species: Human
Sample Types: Cell Culture Supernates
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3D Biomimetic Magnetic Structures for Static Magnetic Field Stimulation of Osteogenesis
Authors: IA Paun, RC Popescu, BS Calin, CC Mustaciosu, M Dinescu, CR Luculescu
Int J Mol Sci, 2018;19(2):.
Species: Human
Sample Types: Cell Culture Supernates
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Macroporous alginate foams crosslinked with strontium for bone tissue engineering
Authors: O Catanzano, A Soriente, A La Gatta, M Cammarota, G Ricci, I Fasolino, C Schiraldi, L Ambrosio, M Malinconic, P Laurienzo, MG Raucci, G Gomez d'Ay
Carbohydr Polym, 2018;202(0):72-83.
Species: Human
Sample Types: Cell Culture Supernates
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Weighted Vest Use during Dietary Weight Loss on Bone Health in Older Adults with Obesity
Authors: JL Kelleher, DP Beavers, RM Henderson, D Yow, C Crotts, J Kiel, BJ Nicklas, KM Beavers
J Osteoporos Phys Act, 2017;5(4):.
Species: Human
Sample Types: Serum
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Undercarboxylated osteocalcin reverts insulin resistance induced by endoplasmic reticulum stress in human umbilical vein endothelial cells
Authors: Q Guo, H Li, L Xu, S Wu, H Sun, B Zhou
Sci Rep, 2017;7(1):46.
Species: Human
Sample Types: Serum
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Identification of suitable reference gene and biomarkers of serum miRNAs for osteoporosis
Sci Rep, 2016;6(0):36347.
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Serum
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Relation between the stability of dental implants and two biological markers during the healing period: a prospective clinical study
Authors: Choknapa Tirachaimo
Int J Implant Dent, 2016;2(1):27.
Species: Human
Sample Types: Crevicular Fluid
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In vitro and in vivo evaluation of zinc-modified ca-si-based ceramic coating for bone implants.
Authors: Yu J, Li K, Zheng X, He D, Ye X, Wang M
PLoS ONE, 2013;8(3):e57564.
Species: Mouse
Sample Types: Cell Culture Supernates
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Acute myeloid leukemia transforms the bone marrow niche into a leukemia-permissive microenvironment through exosome secretion.
Authors: Kumar B, Garcia M, Weng L, Jung X, Murakami J, Hu X, McDonald T, Lin A, Kumar A, DiGiusto D, Stein A, Pullarkat V, Hui S, Carlesso N, Kuo Y, Bhatia R, Marcucci G, Chen C
Leukemia, 0;32(3):575-587.
Species: Human
Sample Types: Plasma
FAQs
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Why can't heparin plasma samples be used with the Human Osteocalcin Quantikine ELISA Kit, Catalog # DSTCN0?
It was found that heparin tends to accelerate the enzymatic breakdown of osteocalcin and it is therefore not recommended with this kit.
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Why is serum not a validated sample type with the Human Osteocalcin Quantikine ELISA Kit, Catalog # DSTCN0?
Serum values have shown to be consistently lower than plasma samples in this kit and therefore are not an ideal sample type.
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Are either of R&D Systems Human Osteocalcin ELISA assays, catalog #s DSTCN0 or DY1419, specific for un-carboxylated or gamma-carboxylated form of Osteocalcin?
The Human Osteocaclin Quantikine ELISA (catalog # DSTCN0) assay recognizes natural and recombinant human Osteocalcin, and the assay recognizes Des-γ-carboxylated-Osteocalcin. The Human Osteocalcin DuoSet ELISA (catalog # DY419) also recognizes natural and recombinant human Osteocalcin, however, we have not determined if the DuoSet also recognizes Des-γ-carboxylated-Osteocalcin.
ELISA Controls
Supplemental ELISA Products
Reviews for Human Osteocalcin Quantikine ELISA Kit
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