Human Osteocalcin Quantikine ELISA Kit

  (6 citations)
(1 Review)
    
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Product Details
Assay Procedure
Citations (6)
FAQs
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), EDTA Plasma (50 uL)
  • Sensitivity
    0.898 ng/mL
  • Assay Range
    2.0 - 64 ng/mL (Cell Culture Supernates, EDTA Plasma)
  • Specificity
    Natural and recombinant human Osteocalcin.
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC137 , Quantikine Immunoassay Control Set 823 for Human Osteocalcin - Please Inquire
Product Summary
The Quantikine Human Osteocalcin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Osteocalcin in cell culture supernates and EDTA plasma. It contains synthetic human Osteocalcin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Osteocalcin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Osteocalcin.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 4 4 4 4 4 4
Mean 8.15 17.2 33.3 9.06 19.6 40
Standard Deviation 0.268 0.395 0.544 0.306 0.379 0.653
CV% 3.3 2.3 1.6 3.4 1.9 1.6

EDTA Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 4 4 4 4 4 4
Mean 9.06 19.6 40 7.91 16.1 33.6
Standard Deviation 0.306 0.379 0.653 0.766 0.991 1.66
CV% 3.4 1.9 1.6 9.7 6.2 4.9

Recovery

The recovery of human Osteocalcin spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 105 98-120
EDTA Plasma (n=4) 102 80-116
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of human Osteocalcin were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
 Osteocalcin [HRP]
Product Datasheets

Certificate of Analysis Lookup

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Certificate of Analysis Lookup

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Note: Certificate of Analysis not available for kit components.

Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Osteocalcin
Osteocalcin, also named Bone gamma-carboxyglutamic Acid Protein, is a secreted protein whose expression is restricted to cells of the osteoblast lineage. It has been frequently used as a marker for osteoblast lineage cells.
  • Long Name:
    Bone gamma-Carboxyglutamate [gla] Protein
  • Entrez Gene IDs:
    632 (Human); 12096 (Mouse); 25295 (Rat)
  • Alternate Names:
    BGLAP; BGP; bone gamma-carboxyglutamate (gla) protein (osteocalcin); bone gamma-carboxyglutamate (gla) protein; Bone Gla protein; Gamma-carboxyglutamic acid-containing protein; OC; OCN; Osteocalcin
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Sample Type
  1. 3D Biomimetic Magnetic Structures for Static Magnetic Field Stimulation of Osteogenesis
    Authors: IA Paun, RC Popescu, BS Calin, CC Mustaciosu, M Dinescu, CR Luculescu
    Int J Mol Sci, 2018;19(2):.
    Species: Human
    Sample Type: Cell Culture Supernates
  2. Undercarboxylated osteocalcin reverts insulin resistance induced by endoplasmic reticulum stress in human umbilical vein endothelial cells
    Authors: Q Guo, H Li, L Xu, S Wu, H Sun, B Zhou
    Sci Rep, 2017;7(1):46.
    Species: Human
    Sample Type: Serum
  3. Weighted Vest Use during Dietary Weight Loss on Bone Health in Older Adults with Obesity
    Authors: JL Kelleher, DP Beavers, RM Henderson, D Yow, C Crotts, J Kiel, BJ Nicklas, KM Beavers
    J Osteoporos Phys Act, 2017;5(4):.
    Species: Human
    Sample Type: Serum
  4. Identification of suitable reference gene and biomarkers of serum miRNAs for osteoporosis
    Sci Rep, 2016;6(0):36347.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Type: Serum
  5. Relation between the stability of dental implants and two biological markers during the healing period: a prospective clinical study
    Authors: Choknapa Tirachaimo
    Int J Implant Dent, 2016;2(1):27.
    Species: Human
    Sample Type: Crevicular Fluid
  6. In vitro and in vivo evaluation of zinc-modified ca-si-based ceramic coating for bone implants.
    Authors: Yu J, Li K, Zheng X, He D, Ye X, Wang M
    PLoS ONE, 2013;8(3):e57564.
    Species: Mouse
    Sample Type: Cell Culture Supernates

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ELISA Controls
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Quantikine Immunoassay Control Set 823 for Human Osteocalcin

Ctrl QC137
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Supplemental ELISA Products
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Quantikine ELISA Wash Buffer 1

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Average Rating: 5 (Based on 1 review)

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