Human p67phox Antibody

Catalog # Availability Size / Price Qty
AF7830
AF7830-SP
Detection of Human p67phox by Western Blot.
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Human p67phox Antibody Summary

Species Reactivity
Human
Specificity
Detects human p67phox in direct ELISAs and Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human p67phox
Lys355-Val526 (His389Gln)
Accession # P19878
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human p67phox by Western Blot. View Larger

Detection of Human p67phox by Western Blot. Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line and human immature dendritic cells. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human p67phox Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7830) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for p67phox at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p67phox

P67phox (67 kDa phagocyte oxidase; also NCF2 and NADPH oxidase activator 2) is a 67-70 kDa cytosolic member of the NCF2/NOXA1 family of proteins. It is expressed in multiple cell types, including B cells, endothelial cells, phagocytic cells such as neutrophils and macrophages and, to some degree, fibroblasts. Upon phagocytosis, phagocytes generate superoxide for the purpose of killing ingested microbes. This occurs through the formation and activation of a complex termed NADPH oxidase, which consists of membrane-associated cytochrome b558, plus Rac GTPase and three SH3 domain-containing components, p47phox and a p67phox:p40-phox dimer. Human p67phox is 526 amino acids (aa) in length. It contains two TPR repeats that participate in scaffold formation (aa 11-98), an SH3 domain (aa 240-299), an OPR motif that regulates PB1 interactions (aa 351-429), and a C-terminal SH3 domain (aa 457-516). There is one splice variant that shows a deletion of aa 231-293, and multiple point mutations along the length of the molecule. Over aa 355-526, human p67phox shares 76% aa identity with mouse p67phox.

Entrez Gene IDs
4688 (Human); 17970 (Mouse); 364018 (Rat)
Alternate Names
autosomal 2; NADPH oxidase activator 2; NCF2; NCF-2; neutrophil cytosol factor 2; neutrophil cytosolic factor 2 (65kD, chronic granulomatous disease, autosomal2); neutrophil cytosolic factor 2; NOXA2; NOXA2FLJ93058; p67phox; p67-phox

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