Human PAG1 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human PAG1 in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human PAG1
    Ser38-Leu432
    Accession # Q9NWQ8
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human PAG1 by Western Blot.

Western blot shows lysates of Raji human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human PAG1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5285) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PAG1 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PAG1
PAG1 (Phosphoprotein-associated with glycosphingolipid-enriched microdomains 1; also Cbp/Csk-binding protein) is a 47 kDa protein that runs anomalously at 70‑85 kDa in SDS-PAGE. PAG1 is a constitutively phosphorylated and palmitoylated protein that occurs in leukocyte lipid rafts. It negatively regulates Src family kinases by linking Csk to FynT. Human PAG1 is 432 amino acids (aa) in length. It is a type III (no signal sequence) transmembrane protein that contains a 16 aa extracellular region (aa 31‑16) and a 395 aa cytoplasmic domain (aa 38‑432). PAG1 is palmitoylated at Cys37 and phosphorylated at Tyr317 where it interacts with Csk. There is an FynT binding site at aa 131‑137. One splice variant shows a 36 aa substitution for aa 293‑432. Over aa 38‑432, human PAG1 is 79% aa identical to mouse PAG1.
  • Long Name:
    Phosphoprotein Associated with Glycosphingolipid Microdomains 1
  • Entrez Gene IDs:
    55824 (Human); 94212 (Mouse)
  • Alternate Names:
    CBP; CBPMGC138364; Csk-binding protein; PAG1; PAGFLJ37858; phosphoprotein associated with glycosphingolipid microdomains 1; phosphoprotein associated with glycosphingolipid-enriched microdomains 1; Transmembrane adapter protein PAG; Transmembrane phosphoprotein Cbp
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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