Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all PAMR1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human PAMR1
Leu22-Lys737
Accession # NP_056245

Specificity

Detects human PAMR1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human PAMR1 Antibody

Detection of Human PAMR1 antibody by Western Blot.

Detection of Human PAMR1 by Western Blot.

Western blot shows lysates of U2OS human osteosarcoma cell line. PVDF Membrane was probed with 1 µg/mL of Human PAMR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6517) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PAMR1 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human PAMR1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: U2OS human osteosarcoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: PAMR1

PAMR1 (Peptidase domain associated with muscle regeneration 1; also RAMP/ regeneration-associated muscle protease homolog) is a secreted, 80 kDa (predicted) member of the peptidase S1 family of enzymes. It is expressed by skeletal muscle, and appears to mediate skeletal muscle regeneration. Due to the lack of a catalytic serine at amino acid (aa) 672, PAMR1 demonstrates no enzyme activity. Mature PAMR1 is 716 aa in length (aa 22-737). It contains a Cys-rich segment (aa 42-94), one CUB domain (aa 128-234), an EGF-like region (aa 235-272), two SUSHI domains (aa 278-444) and a nonfunctional peptidase S1 domain (aa 445-737). There is one 17 aa deletion after Glu276. Over aa 21-737, human PAMR1 shares 88% aa identity with mouse PAMR1.

Long Name

Peptidase Domain Containing Associated with Muscle Regeneration 1

Alternate Names

DKFZP586H2123, RAMP

Entrez Gene IDs

25891 (Human); 210622 (Mouse); 311252 (Rat)

Gene Symbol

PAMR1

UniProt

NP_056245

Additional PAMR1 Products

Product Documents for Human PAMR1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human PAMR1 Antibody

For research use only

Related Research Areas

Serine Proteases and Regulators

Citations for Human PAMR1 Antibody

Customer Reviews for Human PAMR1 Antibody

There are currently no reviews for this product. Be the first to review Human PAMR1 Antibody and earn rewards!

Have you used Human PAMR1 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies