|Detection of Human PAMR1 by Western Blot. Western blot shows lysates of U2OS human osteosarcoma cell line. PVDF Membrane was probed with 1 µg/mL of Human PAMR1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6517) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PAMR1 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
PAMR1 (Peptidase domain associated with muscle regeneration 1; also RAMP/ regeneration-associated muscle protease homolog) is a secreted, 80 kDa (predicted) member of the peptidase S1 family of enzymes. It is expressed by skeletal muscle, and appears to mediate skeletal muscle regeneration. Due to the lack of a catalytic serine at amino acid (aa) 672, PAMR1 demonstrates no enzyme activity. Mature PAMR1 is 716 aa in length (aa 22-737). It contains a Cys-rich segment (aa 42-94), one CUB domain (aa 128-234), an EGF-like region (aa 235-272), two SUSHI domains (aa 278-444) and a nonfunctional peptidase S1 domain (aa 445-737). There is one 17 aa deletion after Glu276. Over aa 21-737, human PAMR1 shares 88% aa identity with mouse PAMR1.
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