Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry, Cell Selection, ELISA Detection

Label

Phycoerythrin (Excitation = 488 nm, Emission = 565-605 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # 130021
View all PD-L1/B7-H1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human B7‑H1/PD‑L1
Phe19-Thr239
Accession # Q9NZQ7

Specificity

Detects human B7‑H1/PD‑L1 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) B7-1, -2, -H2, -H3, -H3b,-H4, rhPD-L2, recombinant mouse B7-H1, recombinant rat (rr) B7-1, or rrB7-2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human PD-L1/B7-H1 PE‑conjugated Antibody

Detection of B7-H1/PD-L1 antibody in Human PBMCs antibody by Flow Cytometry.

Detection of B7-H1/PD-L1 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) untreated (A) or treated (B) overnight with 1 µg/mL LPS were stained with Mouse Anti-Human B7-H1/PD-L1 PE-conjugated Monoclonal Antibody (Catalog # FAB1561P) and Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A). Quadrant markers were set based on control antibody staining (Catalog # IC002P). View our protocol for Staining Membrane-associated Proteins.
Detection of PD-L1/B7-H1 in MDA-MB-231 Human Cell Line by Flow Cytometry.

Detection of PD-L1/B7-H1 in MDA-MB-231 Human Cell Line by Flow Cytometry.

MDA-MB-231 human breast adenocarcinoma cell line was stained with Mouse Anti-Human PD-L1/B7-H1 PE-conjugated Monoclonal Antibody (Catalog # FAB1561P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). Adherent cells were prepared by either manual scraping or with TrypLE Express treatment with similar results. View our protocol for Staining Membrane-associated Proteins.

Applications for Human PD-L1/B7-H1 PE‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: Human peripheral blood mononuclear cells (PBMCs) treated with LPS and MDA-MB-231 human breast adenocarcinoma cell line

Reviewed Applications

Read 1 review rated 4 using FAB1561P in the following applications:

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: PD-L1/B7-H1

Human B7 homolog 1 (B7-H1), also called programmed death ligand 1 (PD-L1) and programmed cell death 1 ligand 1 (PDCD1L1), is a member of the growing B7 family of immune proteins that provide signals for both stimulating and inhibiting T cell activation. Other family members include B7-1, B7-2, B7-H2, PDL2 and B7-H3. B7 proteins are members of the immunoglobulin (Ig) superfamily. Their extracellular domains contain 2 Ig-like domains and all members have short cytoplasmic domains. Among the family members, there is about 20-25% amino acid identity. Human and mouse B7-H1 share approximately 70% amino acid sequence identity. B7-H1 has been identified as one of two ligands for programmed death-1 (PD-1), a member of the CD28 family of immunoreceptors. The B7-H1 gene encodes a 291 amino acid (aa) type I membrane precursor protein with a putative 18 aa signal peptide, a 220 aa extracellular domain, a 21 aa transmembrane region, and a 31 aa cytoplasmic domain. Human B7-H1 is constitutively expressed in several organs such as heart, skeletal muscle, placenta and lung, and in lower amounts in thymus, spleen, kidney and liver. B7-H1 expression is upregulated in a small fraction of activated T and B cells and a much larger fraction of activated monocytes. B7-H1 expression is also induced in dendritic cells and keratinocytes after IFN-gamma stimulation. Interaction of B7-H1 with PD-1 results in inhibition of TCR-mediated proliferation and cytokine production. The B7-H1:PD-1 pathway is involved in the negative regulation of some immune responses and may play an important role in the regulation of peripheral tolerance.

References

  1. Nishimura, H. and T. Honjo (2001) Trends Immunol. 22:265.
  2. Freeman, G.J. et al. (2000) J. Exp. Med. 192:1027.
  3. Latchman, Y. et al. (2001) Nat. Immunol. 2:261.

Long Name

Programmed Death Ligand 1

Alternate Names

B7-H1, B7H1, CD274, PDCD1L1, PDCD1LG1, PDL1

Entrez Gene IDs

29126 (Human); 60533 (Mouse); 499342 (Rat); 574058 (Porcine); 484186 (Canine); 102145573 (Cynomolgus Monkey)

Gene Symbol

CD274

UniProt

Q9NZQ7

Additional PD-L1/B7-H1 Products

Product Documents for Human PD-L1/B7-H1 PE‑conjugated Antibody

Certificate of Analysis

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Product Specific Notices for Human PD-L1/B7-H1 PE‑conjugated Antibody

For research use only

Citations for Human PD-L1/B7-H1 PE‑conjugated Antibody

Customer Reviews for Human PD-L1/B7-H1 PE‑conjugated Antibody (1)

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  • Human B7-H1/PD-L1 PE-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Melanoma tissue
    Species: Human
    Verified Customer | Posted 12/03/2016
    FEMX Human melanoma cells (1x10^6) were incubated for 30 minutes at 4C with 10 ul of Mouse B7-H1/PD-L1 PE-conjugated antibody (red), or PE-conjugated Mouse IgG1 (gray). Blocking: 1% BSA. Unfixed cells.
    Human PD-L1/B7-H1 PE‑conjugated Antibody FAB1561P

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