Human PD-L2/B7-DC Antibody

Catalog # Availability Size / Price Qty
Detection of PD‑L2 in HepG2 Human Cell Line by Flow Cytometry.
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Product Details
Citations (13)
Supplemental Products
Reviews (2)

Human PD-L2/B7-DC Antibody Summary

Species Reactivity
Detects human PD-L2/B7-DC in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse PD-L2, recombinant human (rh) B7-1, rhB7-2, rhB7-H1, rhB7-H2, or rhB7-H3 is observed.
Monoclonal Mouse IgG2B Clone # 176611
Protein A or G purified from hybridoma culture supernatant
Mouse myeloma cell line NS0-derived recombinant human PD-L2/B7-DC
Leu20-Pro219 (predicted)
Accession # Q9BQ51
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
Recombinant Human PD-L2 Fc Chimera (Catalog # 1224-PL)
Flow Cytometry
2.5 µg/106 cells
See below
0.5-25 µg/mL
See below
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of PD‑L2 antibody in HepG2 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of PD‑L2 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Human PD-L2 Monoclonal Antibody (Catalog # MAB1224, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # F0102B).

Immunohistochemistry PD-L2/B7-DC antibody in Human Lung Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

PD‑L2/B7‑DC in Human Lung Cancer Tissue. PD-L2/B7-DC was detected in immersion fixed paraffin-embedded sections of human lung cancer tissue using Mouse Anti-Human PD-L2/B7-DC Monoclonal Antibody (Catalog # MAB1224) at 0.5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PD-L2/B7-DC

T cells require a signal induced by the engagement of the T cell receptor and a “co‑stimulatory” signal(s) through distinct T cell surface molecules for optimal T cell activation and tolerance. Members of the B7 superfamily of counter-receptors were identified by their ability to interact with co‑stimulatory molecules found on the surface of T cells. Members of the B7 superfamily include B7-1 (CD80), B7-2 (CD86), B7-H1 (PD-L1), B7-H2 (B7RP-1), B7-H3, and PD-L2 (B7-DC) (1). B7 proteins are immunoglobulin (Ig) superfamily members with extracellular Ig-V-like and Ig-C-like domains and short cytoplasmic domains. Among the family members, they share from 20‑40% amino acid (aa) sequence identity. The cloned human PD-L2 cDNA encodes a 273 aa type I membrane precursor protein with a putative 20 aa signal peptide, a 201 aa extracellular region containing one V-like and one C-like Ig domain, a 24 aa transmembrane region, and a 28 aa cytoplasmic domain. The extracellular domains of mouse and human PD-L2 share approximately 70% aa sequence identity (2). PD-L2 is one of two ligands for programmed death-1 (PD-1), a member of the CD28 family of immuno-receptors. The other identified ligand is PD-L1. Human PD-L1 and PD-L2 share approximately 41% aa sequence identity and have similar functions. PD-L2 is broadly expressed in tissues. Highest expression was detected by Northern blot analysis in heart, placenta, liver, pancreas, spleen, and lymph node. Lower amounts of expression were observed in lung, smooth muscle, and thymus. Expression of PD-L2 on antigen presenting cell has been examined in detail. Resting B cells, monocytes and dendritic cells do not express PD-L2, expression however can be induced by LPS or BCR activation in B cells, INF-gamma treatment in monocytes, or LPS plus IFN-gamma treatment of dendritic cells. PD-L2 expression is also up regulated in a variety of tumor cell lines. On previously activated T cells, PD-L2 interaction with PD-1 inhibits TCR-mediated proliferation and cytokine production, suggesting an inhibitory role in regulating immune responses. In contrast, a co‑stimulatory function for the PD-L2 on resting T cells activated with sub-optimal TCR signals has also been reported (3).


  1. Coyle, A.J. and J-C. Gutierrrez-Ramos (2001) Nature Immunol. 2:203.
  2. Latchman Y. et al. (2001) Nature Immun. 2:261.
  3. Carreno, B.M. and M. Collins (2002) Annu. Rev. Immunol. 20:29.
Long Name
Programmed Death-1 Ligand-2
Entrez Gene IDs
80380 (Human); 58205 (Mouse); 309304 (Rat); 102146227 (Cynomolgus Monkey)
Alternate Names
B7-DC; bA574F11.2; Btdc; Butyrophilin B7-DC; Butyrophilin-like Protein; CD273 antigen; CD273; CD273PD-1 ligand 2; MGC142240; PD-1-ligand 2; PDCD1L2MGC142238; PDCD1LG2; PDL2; PD-L2; PDL2B7DC; PD-L2PDCD1 ligand 2; programmed cell death 1 ligand 2; Programmed death ligand 2

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Citations for Human PD-L2/B7-DC Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

13 Citations: Showing 1 - 10
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  1. Nicotine treatment regulates PD-L1 and PD-L2 expression via inhibition of Akt pathway in HER2-type breast cancer cells
    Authors: MA Murayama, E Takada, K Takai, N Arimitsu, J Shimizu, T Suzuki, N Suzuki
    PLoS ONE, 2022;17(1):e0260838.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Radiotherapy Combined with PD-1 Inhibition Increases NK Cell Cytotoxicity towards Nasopharyngeal Carcinoma Cells
    Authors: A Makowska, N Lelabi, C Nothbaum, L Shen, P Busson, TTB Tran, M Eble, U Kontny
    Cells, 2021;10(9):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Changes in immune parameters between pre-treatment and recurrence after (chemo) radiation therapy in patients with head and neck cancer
    Authors: T Ono, K Azuma, A Kawahara, T Kakuma, F Sato, T Kawaguchi, J Akiba, H Umeno
    Sci Rep, 2020;10(1):11973.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  4. MET Receptor Tyrosine Kinase Regulates the Expression of Co-Stimulatory and Co-Inhibitory Molecules in Tumor Cells and Contributes to PD-L1-Mediated Suppression of Immune Cell Function
    Authors: HK Ahn, S Kim, D Kwon, J Koh, YA Kim, K Kim, DH Chung, YK Jeon
    Int J Mol Sci, 2019;20(17):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. PD-L1 expression and presence of TILs in small intestinal neuroendocrine tumours
    Authors: A Lamarca, D Nonaka, W Breitwiese, G Ashton, J Barriuso, MG McNamara, S Moghadam, J Rogan, W Mansoor, RA Hubner, C Clark, B Chakrabart, JW Valle
    Oncotarget, 2018;9(19):14922-14938.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  6. Cancer-associated fibroblasts induce antigen-specific deletion of CD8+T Cells to protect tumour cells
    Authors: MA Lakins, E Ghorani, H Munir, CP Martins, JD Shields
    Nat Commun, 2018;9(1):948.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Classification of gallbladder cancer by assessment of CD8+ TIL and PD-L1 expression
    Authors: J Lin, J Long, X Wan, J Chen, Y Bai, A Wang, X Yang, Y Wu, SC Robson, X Sang, H Zhao
    BMC Cancer, 2018;18(1):766.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. Programmed death-1 (PD-1) expression in cervical intraepithelial neoplasia and its relationship with recurrence after conization
    Authors: H Chang, JH Hong, JK Lee, HW Cho, YT Ouh, KJ Min, KA So
    J Gynecol Oncol, 2018;0(0):.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  9. Variation in nuclear size and PD-L2 positivity correlate with aggressive chromophobe renal cell carcinoma
    Authors: ME Mostafa, A Abdelkader, N Kuroda, D Pérez-Mont, A Banerjee, O Hes, KA Iczkowski
    Ann Diagn Pathol, 2018;34(0):31-35.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  10. PD-L1 is a Prognostic Biomarker in Resected NSCLC Patients with Moderate/high Smoking History and Elevated Serum SCCA Level
    Authors: L Cao, X Wang, S Li, Q Zhi, Y Wang, L Wang, K Li, R Jiang
    J Cancer, 2017;8(16):3251-3260.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  11. Clinicopathologic analysis of programmed cell death-1 and programmed cell death-ligand 1 and 2 expressions in pulmonary adenocarcinoma: comparison with histology and driver oncogenic alteration status.
    Authors: Koh J, Go H, Keam B, Kim M, Nam S, Kim T, Lee S, Min H, Kim Y, Kim D, Jeon Y, Chung D
    Mod Pathol, 2015;28(9):1154-66.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  12. IFN-gamma generates maturation-arrested dendritic cells that induce T cell hyporesponsiveness independent of Foxp3+ T-regulatory cell generation.
    Authors: Rojas D, Krishnan R,
    Immunol. Lett., 2010;132(1):31-7.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Expression of B7-H1 on gastric epithelial cells: its potential role in regulating T cells during Helicobacter pylori infection.
    Authors: Das S, Suarez G, Beswick EJ, Sierra JC, Graham DY, Reyes VE
    J. Immunol., 2006;176(5):3000-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot


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Human PD-L2/B7-DC Antibody
By Anonymous on 10/06/2021
Application: WB Sample Tested: Lung tissue Species: Human

Human PD-L2/B7-DC Antibody
By Masahiro Hirata on 04/01/2021
Application: IHC Sample Tested: Adult lung Species: Human

working concentration: 0.5ug/mL / BSA-TBS
epitope retrieval in ER2(Leica)
Leica BOND Refine Detection kit