Human PDGF-AB Quantikine ELISA Kit

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PDHD00C
SHD00C
DHD00C
Control Products Available
Human PDGF-AB ELISA Cell Culture Supernate Standard Curve
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Product Details
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Citations (40)
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Human PDGF-AB Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (10 uL), Platelet-poor EDTA Plasma (100 uL), Platelet-poor Heparin Plasma (100 uL)
Sensitivity
3.83 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma)
Specificity
Natural and recombinant human PDGF-AB
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
Interference observed with 1 or more available related molecules.

Product Summary

The Quantikine PDGF-AB Immunoassay is a 4.5 hour solid phase ELISA designed to measure human PDGF-AB in cell culture supernates, serum, and platelet-poor plasma. It contains E. coli-expressed recombinant human PDGF-AB and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human PDGF-AB showed linear curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human PDGF-AB.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 92.7 312 656 102 343 701
Standard Deviation 8.28 9.73 15.2 10.9 29.9 52.3
CV% 8.9 3.1 2.3 10.7 8.7 7.5

Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 107 350 726 119 362 723
Standard Deviation 6.95 10.9 63.1 10.7 20.9 54.5
CV% 6.5 3.1 8.7 9 5.8 7.5

Recovery

The recovery of PDGF-AB spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 105 93-119
Platelet-poor EDTA Plasma (n=4) 104 78-117
Platelet-poor Heparin Plasma (n=4) 105 86-121

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of PDGF-AB were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human PDGF-AB ELISA Linearity

Scientific Data

Human PDGF-AB ELISA Cell Culture Supernate Standard Curve

Human PDGF-AB ELISA Serum/Plasma Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PDGF-AB

The platelet-derived growth factor (PDGF) family of disulfide-linked dimeric proteins consists of four homodimeric proteins, PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD, and one heterodimeric protein, PDGF-AB. Members of this protein family act mainly on connective tissue. The dimeric isoforms of PDGF are expressed by different cell types and at different times during embryonic development. All PDGF isoforms are synthesized as inactive precursors and are processed to active forms that contain a PDGF/VEGF cysteine knot domain. Only PDGF-C and PDGF-D, also named spinal cord-derived growth factors SCDGF and SCDGF-B, also contain an N-terminal CUB domain. Mature PDGF dimers bind and induce the homo- or hetero-dimerization of two receptor tyrosine kinases (PDGF R alpha and R beta).

Long Name:
Platelet-derived Growth Factor AB
Alternate Names:
PDGFAB; PDGF-AB
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 100 µL Standard, Control, or Sample
  6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human PDGF-AB Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

40 Citations: Showing 1 - 10
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  1. Plateletcrit and Mean Platelet Volume in the Evaluation of Alcoholic Liver Cirrhosis and Nonalcoholic Fatty Liver Disease Patients
    Authors: A Michalak, H Cicho?-Lac, M Guz, J Kozicka, M Cybulski, W Jeleniewic
    BioMed Research International, 2021;2021(0):8867985.
    Species: Human
    Sample Types: Serum
  2. Circulating cytokines present in multiple myeloma patients inhibit the osteoblastic differentiation of adipose stem cells
    Authors: L Kobari, M Auclair, O Piau, N Ferrand, M Zaoui, F Delhommeau, B Fève, M Sabbah, L Garderet
    Leukemia, 2021;0(0):.
    Species: Human
    Sample Types: Plasma
  3. Controlled Growth Factor Delivery and Cyclic Stretch Induces a Smooth Muscle Cell-like Phenotype in Adipose-Derived Stem Cells
    Authors: B Walters, PA Turner, B Rolauffs, ML Hart, JP Stegemann
    Cells, 2021;10(11):.
    Species: Human
    Sample Types: Whole Cells
  4. In Ovarian Cancer Multicellular Spheroids, Platelet Releasate Promotes Growth, Expansion of ALDH+ and CD133+ Cancer Stem Cells, and Protection against the Cytotoxic Effects of Cisplatin, Carboplatin and Paclitaxel
    Authors: N Casagrande, C Borghese, F Agostini, C Durante, M Mazzucato, A Colombatti, D Aldinucci
    International Journal of Molecular Sciences, 2021;22(6):.
    Species: Human
    Sample Types: Plasma
  5. Towards an evaluation of alcoholic liver cirrhosis and nonalcoholic fatty liver disease patients with hematological scales
    Authors: A Michalak, H Cicho?-Lac, M Guz, J Kozicka, M Cybulski, W Jeleniewic, A Stepulak
    World Journal of Gastroenterology, 2020;26(47):7538-7549.
    Species: Human
    Sample Types: Serum
  6. 1H-NMR and MALDI-TOF MS as metabolomic quality control tests to classify platelet derived medium additives for GMP compliant cell expansion procedures
    Authors: F Agostini, M Ruzza, D Corpillo, L Biondi, E Acquadro, B Canepa, A Viale, M Battiston, F Serra, S Aime, M Mazzucato
    PLoS ONE, 2018;13(9):e0203048.
    Species: Human
    Sample Types: Cell Culture Supernates
  7. A novel dressing for the combined delivery of platelet lysate and vancomycin hydrochloride to chronic skin ulcers: Hyaluronic acid particles in alginate matrices
    Authors: S Rossi, M Mori, B Vigani, MC Bonferoni, G Sandri, F Riva, C Caramella, F Ferrari
    Eur J Pharm Sci, 2018;118(0):87-95.
  8. The effect of autologous platelet rich plasma on tenocytes of the human rotator cuff
    Authors: S Pauly, F Klatte-Sch, K Stahnke, M Scheibel, B Wildemann
    BMC Musculoskelet Disord, 2018;19(1):422.
    Species: Human
    Sample Types: Plasma
  9. Preparing Platelet-Rich Plasma with Whole Blood Harvested Intraoperatively During Spinal Fusion
    Authors: B Shen, Z Zhang, NF Zhou, YF Huang, YJ Bao, DS Wu, YD Zhang
    Med. Sci. Monit., 2017;23(0):3578-3584.
    Species: Human
    Sample Types: Plasma
  10. Cardiac rehabilitation programme as a non-pharmacological platelet inhibitory tool in acute coronary syndrome survivors
    Authors: E Tóth-Zsámb, Z Horváth, L Hajtman, S Leé, É Pállinger, E Kuklis, Á Tahy, G Fekete, L Kohut, RG Kiss
    Eur J Prev Cardiol, 2017;0(0):2047487317704.
    Species: Human
    Sample Types: Plasma
  11. Umbilical cord extracts improve diabetic abnormalities in bone marrow-derived mesenchymal stem cells and increase their therapeutic effects on diabetic nephropathy
    Authors: K Nagaishi, Y Mizue, T Chikenji, M Otani, M Nakano, Y Saijo, H Tsuchida, S Ishioka, A Nishikawa, T Saito, M Fujimiya
    Sci Rep, 2017;7(1):8484.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. The production method affects the efficacy of platelet derivatives to expand mesenchymal stromal cells in vitro
    Authors: M Bernardi, F Agostini, K Chieregato, E Amati, C Durante, M Rassu, M Ruggeri, S Sella, E Lombardi, M Mazzucato, G Astori
    J Transl Med, 2017;15(1):90.
    Species: Human
    Sample Types: Cell Lysates
  13. Human neural stem cell-induced endothelial morphogenesis requires autocrine/paracrine and juxtacrine signaling
    Sci Rep, 2016;6(0):29029.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Biological effects of plasma rich in growth factors (PRGF) on human endometrial fibroblasts
    Authors: Gorka Orive
    Eur. J. Obstet. Gynecol. Reprod. Biol., 2016;206(0):125-130.
    Species: Human
    Sample Types: Plasma
  15. Overcoming the bottleneck of platelet lysate supply in large-scale clinical expansion of adipose-derived stem cells: A comparison of fresh versus three types of platelet lysates from outdated buffy coat-derived platelet concentrates
    Authors: Anne Fischer-Ni
    Cytotherapy, 2016;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. Involvement of Rac in thromboxane A2induced human platelet activation: regulation of sCD40 ligand release and PDGFAB secretion.
    Authors: Kageyama Y, Doi T, Matsushima-Nishiwaki R, Iida Y, Akamatsu S, Kondo A, Kuroyanagi G, Yamamoto N, Mizutani J, Otsuka T, Tokuda H, Iida H, Kozawa O, Ogura S
    Mol Med Rep, 2014;10(1):107-12.
    Species: Human
    Sample Types: Plasma
  17. Ex vivo expansion of bovine corneal endothelial cells in xeno-free medium supplemented with platelet releasate.
    Authors: Chou M, Burnouf T, Wang T
    PLoS ONE, 2014;9(6):e99145.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. Pathogen-free, plasma-poor platelet lysate and expansion of human mesenchymal stem cells.
    Authors: Iudicone, Paola, Fioravanti, Daniela, Bonanno, Giuseppi, Miceli, Michelin, Lavorino, Claudio, Totta, Pierange, Frati, Luigi, Nuti, Marianna, Pierelli, Luca
    J Transl Med, 2014;12(0):28.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. Functional and differential proteomic analyses to identify platelet derived factors affecting ex vivo expansion of mesenchymal stromal cells.
    Authors: Kinzebach S, Dietz L, Kluter H, Thierse H, Bieback K
    BMC Cell Biol, 2013;14(0):48.
    Species: Human
    Sample Types: Cell Lysates
  20. Effect of platelet lysate on human cells involved in different phases of wound healing.
    Authors: Barsotti M, Losi P, Briganti E, Sanguinetti E, Magera A, Al Kayal T, Feriani R, Di Stefano R, Soldani G
    PLoS ONE, 2013;8(12):e84753.
    Species: Human
    Sample Types: Cell Lysates
  21. Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media.
    Authors: Rauch C, Feifel E, Amann EM, Spotl HP, Schennach H, Pfaller W, Gstraunthaler G
    ALTEX, 2011;28(4):305-16.
    Species: Human
    Sample Types: Cell Lysates
  22. Platelet-derived growth factor (PDGF)-PDGF receptor interaction activates bone marrow-derived mesenchymal stromal cells derived from chronic lymphocytic leukemia: implications for an angiogenic switch.
    Authors: Ding W, Knox TR, Tschumper RC
    Blood, 2010;116(16):2984-93.
    Species: Human
    Sample Types: Platelet Poor Plasma
  23. Dynamic change in phosphorylated platelet-derived growth factor receptor in peripheral blood leukocytes following docetaxel therapy predicts progression-free and overall survival in prostate cancer.
    Authors: Mathew P, Thall PF, Wen S, Bucana C, Jones D, Horne E, Oh WK, Morris MJ, Lee YC, Logothetis CJ, Lin SH, Fidler IJ
    Br. J. Cancer, 2008;99(9):1426-32.
    Species: Human
    Sample Types: Plasma
  24. Oncogenic and angiogenic growth factors accumulate during routine storage of apheresis platelet concentrates.
    Authors: Kanter J, Khan SY, Kelher M, Gore L, Silliman CC
    Clin. Cancer Res., 2008;14(12):3942-7.
    Species: Human
    Sample Types: Platelet Poor Plasma
  25. Selective cyclooxygenase-2 inhibitor downregulates the paracrine epithelial-mesenchymal interactions of growth in scirrhous gastric carcinoma.
    Authors: Yashiro M, Nakazawa K, Tendo M, Kosaka K, Shinto O, Hirakawa K
    Int. J. Cancer, 2007;120(3):686-93.
    Species: Human
    Sample Types: Cell Culture Supernates
  26. TF/FVIIa transactivate PDGFRbeta to regulate PDGF-BB-induced chemotaxis in different cell types: involvement of Src and PLC.
    Authors: Siegbahn A, Johnell M, Nordin A, Aberg M, Velling T
    Arterioscler. Thromb. Vasc. Biol., 2007;28(1):135-41.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  27. Eosinophil-derived cationic proteins activate the synthesis of remodeling factors by airway epithelial cells.
    Authors: Pegorier S, Wagner LA, Gleich GJ, Pretolani M
    J. Immunol., 2006;177(7):4861-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  28. Tumor-driven paracrine platelet-derived growth factor receptor alpha signaling is a key determinant of stromal cell recruitment in a model of human lung carcinoma.
    Authors: Tejada ML, Yu L, Dong J, Jung K, Meng G, Peale FV, Frantz GD, Hall L, Liang X, Gerber HP, Ferrara N
    Clin. Cancer Res., 2006;12(9):2676-88.
    Species: Human
    Sample Types: Cell Culture Supernates
  29. Expression of PDGF and their receptors in human retinal pigment epithelial cells and fibroblasts: regulation by TGF-beta.
    Authors: Nagineni CN, Kutty V, Detrick B, Hooks JJ
    J. Cell. Physiol., 2005;203(1):35-43.
    Species: Human
    Sample Types: Cell Culture Supernates
  30. Autologous fibrin glue with growth factors in reconstructive maxillofacial surgery.
    Authors: Thorn JJ, Sorensen H, Weis-Fogh U, Andersen M
    Int J Oral Maxillofac Surg, 2004;33(1):95-100.
    Species: Human
    Sample Types: Complex Sample Type
  31. Experimental study of the effect of platelet-rich plasma on osteogenesis in rabbit.
    Authors: Zhang CQ, Yuan T, Zeng BF
    Chin. Med. J., 2004;117(12):1853-5.
    Species: Rabbit
    Sample Types: Plasma
  32. Smad3 is required for dedifferentiation of retinal pigment epithelium following retinal detachment in mice.
    Authors: Saika S, Kono-Saika S, Tanaka T, Yamanaka O, Ohnishi Y, Sato M, Muragaki Y, Ooshima A, Yoo J, Flanders KC, Roberts AB
    Lab. Invest., 2004;84(10):1245-58.
    Species: Human
    Sample Types: Cell Culture Supernates
  33. Fibrinogen induces chemotactic activity in endothelial cells.
    Authors: Seeger FH, U£3+jV&#x12;—I¬, Blessing E, Gu L, Bornhold R, Denger S, Kreuzer J
    , 2002;176(2):109-15.
    Species: Porcine
    Sample Types: Cell Culture Supernates
  34. Ovariectomy increases mitogens and platelet-induced proliferation of arterial smooth muscle.
    Authors: Bracamonte MP, Rud KS, Owen WG, Miller VM
    Am. J. Physiol. Heart Circ. Physiol., 2002;283(3):H853-60.
    Species: Porcine
    Sample Types: Cell Lysates
  35. Cyclic mechanical stretching modulates secretion pattern of growth factors in human tendon fibroblasts.
    Authors: Skutek M, van Griensven M, Zeichen J, Brauer N, Bosch U
    Eur. J. Appl. Physiol., 2001;86(1):48-52.
    Species: Human
    Sample Types: Cell Culture Supernates
  36. Angiogenic balance in human melanoma: expression of VEGF, bFGF, IL-8, PDGF and angiostatin in relation to vascular density of xenografts in vivo.
    Authors: Westphal JR, Van't Hullenaar R, Peek R, Willems RW, Crickard K, Crickard U, Askaa J, Clemmensen I, Ruiter DJ, De Waal RM
    Int. J. Cancer, 2000;86(6):768-76.
    Species: Human
    Sample Types: Cell Culture Supernates
  37. Quantification of growth factor levels using a simplified method of platelet-rich plasma gel preparation.
    Authors: Landesberg R, Roy M, Glickman RS
    J. Oral Maxillofac. Surg., 2000;58(3):297.
    Species: Human
    Sample Types: Plasma
  38. Platelet-derived growth factor (PDGF-AB) like immune reactivity in serum and in cerebral spinal fluid following experimental subarachnoid haemorrhage in dogs.
    Authors: Vieweg U, 49315, Schramm J, Urbach H
    Acta Neurochir (Wien), 1999;141(8):861-5.
    Species: Canine
    Sample Types: Serum
  39. Growth factors and proinflammatory cytokines in the renal involvement of POEMS syndrome.
    Authors: Soubrier M, Sauron C, Souweine B, Larroche C, Wechsler B, Guillevin L, Piette JC, Rousset H, Deteix P
    Am. J. Kidney Dis., 1999;34(4):633-8.
    Species: Human
    Sample Types: Serum
  40. Hypoxia-induced expression of VEGF is reversible in myocardial vascular smooth muscle cells.
    Authors: Gu JW, Adair TH
    Am. J. Physiol., 1997;273(2):H628-33.
    Species: Canine
    Sample Types: Cell Culture Supernates

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