• Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (100 uL), Serum (10 uL), Platelet-poor EDTA Plasma (100 uL), Platelet-poor Heparin Plasma (100 uL)
  • Sensitivity
    3.83 pg/mL
  • Assay Range
    15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma)
  • Specificity
    Natural and recombinant human PDGF-AB
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    Interference observed with 1 or more available related molecules.
Control Available
QC20, Quantikine Immunoassay Control Group 3 - Please Inquire
Product Summary
The Quantikine PDGF-AB Immunoassay is a 4.5 hour solid phase ELISA designed to measure human PDGF-AB in cell culture supernates, serum, and platelet-poor plasma. It contains E. coli-expressed recombinant human PDGF-AB and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human PDGF-AB showed linear curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human PDGF-AB.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
Intra-Assay Precision Inter-Assay Precision
Standard Deviation8.287.816.69.7315.232.710.914.133.129.952.365.7

Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Standard Deviation6.9510.963.110.720.954.5

Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Standard Deviation7.6824.924.716.837.968.4


The recovery of PDGF-AB spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 105 93-119
Platelet-poor EDTA Plasma (n=4) 104 78-117
Platelet-poor Heparin Plasma (n=4) 105 86-121
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of PDGF-AB were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human PDGF-AB Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: PDGF-AB
The platelet-derived growth factor (PDGF) family of disulfide-linked dimeric proteins consists of four homodimeric proteins, PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD, and one heterodimeric protein, PDGF-AB. Members of this protein family act mainly on connective tissue. The dimeric isoforms of PDGF are expressed by different cell types and at different times during embryonic development. All PDGF isoforms are synthesized as inactive precursors and are processed to active forms that contain a PDGF/VEGF cysteine knot domain. Only PDGF-C and PDGF-D, also named spinal cord-derived growth factors SCDGF and SCDGF-B, also contain an N-terminal CUB domain. Mature PDGF dimers bind and induce the homo- or hetero-dimerization of two receptor tyrosine kinases (PDGF R alpha and R beta).
    • Long Name
      Platelet-derived Growth Factor AB
    • Alternate Names
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 100 µL Standard, Control, or Sample
    6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 20 minutes on the benchtop. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    29 Citations: Showing 1 - 10
    Filter your results:

    Sample Type
    1. Preparing Platelet-Rich Plasma with Whole Blood Harvested Intraoperatively During Spinal Fusion
      Authors: B Shen, Z Zhang, NF Zhou, YF Huang, YJ Bao, DS Wu, YD Zhang
      Med. Sci. Monit., 2017;23(0):3578-3584.
      Species: Human
      Sample Type: Plasma
    2. The production method affects the efficacy of platelet derivatives to expand mesenchymal stromal cells in vitro
      Authors: M Bernardi, F Agostini, K Chieregato, E Amati, C Durante, M Rassu, M Ruggeri, S Sella, E Lombardi, M Mazzucato, G Astori
      J Transl Med, 2017;15(1):90.
      Species: Human
      Sample Type: Cell Lysates
    3. Umbilical cord extracts improve diabetic abnormalities in bone marrow-derived mesenchymal stem cells and increase their therapeutic effects on diabetic nephropathy
      Authors: K Nagaishi, Y Mizue, T Chikenji, M Otani, M Nakano, Y Saijo, H Tsuchida, S Ishioka, A Nishikawa, T Saito, M Fujimiya
      Sci Rep, 2017;7(1):8484.
      Species: Human
      Sample Type: Cell Culture Supernates
    4. Human neural stem cell-induced endothelial morphogenesis requires autocrine/paracrine and juxtacrine signaling
      Sci Rep, 2016;6(0):29029.
      Species: Human
      Sample Type: Cell Culture Supernates
    5. Pathogen-free, plasma-poor platelet lysate and expansion of human mesenchymal stem cells.
      Authors: Iudicone, Paola, Fioravanti, Daniela, Bonanno, Giuseppi, Miceli, Michelin, Lavorino, Claudio, Totta, Pierange, Frati, Luigi, Nuti, Marianna, Pierelli, Luca
      J Transl Med, 2014;12(0):28.
      Species: Human
      Sample Type: Cell Culture Supernates
    6. Ex vivo expansion of bovine corneal endothelial cells in xeno-free medium supplemented with platelet releasate.
      Authors: Chou M, Burnouf T, Wang T
      PLoS ONE, 2014;9(6):e99145.
      Species: Human
      Sample Type: Cell Culture Supernates
    7. Involvement of Rac in thromboxane A2induced human platelet activation: regulation of sCD40 ligand release and PDGFAB secretion.
      Authors: Kageyama Y, Doi T, Matsushima-Nishiwaki R, Iida Y, Akamatsu S, Kondo A, Kuroyanagi G, Yamamoto N, Mizutani J, Otsuka T, Tokuda H, Iida H, Kozawa O, Ogura S
      Mol Med Rep, 2014;10(1):107-12.
      Species: Human
      Sample Type: Plasma
    8. Functional and differential proteomic analyses to identify platelet derived factors affecting ex vivo expansion of mesenchymal stromal cells.
      Authors: Kinzebach S, Dietz L, Kluter H, Thierse H, Bieback K
      BMC Cell Biol, 2013;14(0):48.
      Species: Human
      Sample Type: Cell Lysates
    9. Effect of platelet lysate on human cells involved in different phases of wound healing.
      Authors: Barsotti M, Losi P, Briganti E, Sanguinetti E, Magera A, Al Kayal T, Feriani R, Di Stefano R, Soldani G
      PLoS ONE, 2013;8(12):e84753.
      Species: Human
      Sample Type: Cell Lysates
    10. Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media.
      Authors: Rauch C, Feifel E, Amann EM, Spotl HP, Schennach H, Pfaller W, Gstraunthaler G
      ALTEX, 2011;28(4):305-16.
      Species: Human
      Sample Type: Cell Lysates
    11. Platelet-derived growth factor (PDGF)-PDGF receptor interaction activates bone marrow-derived mesenchymal stromal cells derived from chronic lymphocytic leukemia: implications for an angiogenic switch.
      Authors: Ding W, Knox TR, Tschumper RC
      Blood, 2010;116(16):2984-93.
      Species: Human
      Sample Type: Platelet-Poor Plasma
    12. Dynamic change in phosphorylated platelet-derived growth factor receptor in peripheral blood leukocytes following docetaxel therapy predicts progression-free and overall survival in prostate cancer.
      Authors: Mathew P, Thall PF, Wen S, Bucana C, Jones D, Horne E, Oh WK, Morris MJ, Lee YC, Logothetis CJ, Lin SH, Fidler IJ
      Br. J. Cancer, 2008;99(9):1426-32.
      Species: Human
      Sample Type: Plasma
    13. Oncogenic and angiogenic growth factors accumulate during routine storage of apheresis platelet concentrates.
      Authors: Kanter J, Khan SY, Kelher M, Gore L, Silliman CC
      Clin. Cancer Res., 2008;14(12):3942-7.
      Species: Human
      Sample Type: Platelet-Poor Plasma
    14. TF/FVIIa transactivate PDGFRbeta to regulate PDGF-BB-induced chemotaxis in different cell types: involvement of Src and PLC.
      Authors: Siegbahn A, Johnell M, Nordin A, Aberg M, Velling T
      Arterioscler. Thromb. Vasc. Biol., 2007;28(1):135-41.
      Species: Porcine
      Sample Type: Cell Culture Supernates
    15. Selective cyclooxygenase-2 inhibitor downregulates the paracrine epithelial-mesenchymal interactions of growth in scirrhous gastric carcinoma.
      Authors: Yashiro M, Nakazawa K, Tendo M, Kosaka K, Shinto O, Hirakawa K
      Int. J. Cancer, 2007;120(3):686-93.
      Species: Human
      Sample Type: Cell Culture Supernates
    16. Eosinophil-derived cationic proteins activate the synthesis of remodeling factors by airway epithelial cells.
      Authors: Pegorier S, Wagner LA, Gleich GJ, Pretolani M
      J. Immunol., 2006;177(7):4861-9.
      Species: Human
      Sample Type: Cell Culture Supernates
    17. Tumor-driven paracrine platelet-derived growth factor receptor alpha signaling is a key determinant of stromal cell recruitment in a model of human lung carcinoma.
      Authors: Tejada ML, Yu L, Dong J, Jung K, Meng G, Peale FV, Frantz GD, Hall L, Liang X, Gerber HP, Ferrara N
      Clin. Cancer Res., 2006;12(9):2676-88.
      Species: Human
      Sample Type: Cell Culture Supernates
    18. Expression of PDGF and their receptors in human retinal pigment epithelial cells and fibroblasts: regulation by TGF-beta.
      Authors: Nagineni CN, Kutty V, Detrick B, Hooks JJ
      J. Cell. Physiol., 2005;203(1):35-43.
      Species: Human
      Sample Type: Cell Culture Supernates
    19. Smad3 is required for dedifferentiation of retinal pigment epithelium following retinal detachment in mice.
      Authors: Saika S, Kono-Saika S, Tanaka T, Yamanaka O, Ohnishi Y, Sato M, Muragaki Y, Ooshima A, Yoo J, Flanders KC, Roberts AB
      Lab. Invest., 2004;84(10):1245-58.
      Species: Human
      Sample Type: Cell Culture Supernates
    20. Experimental study of the effect of platelet-rich plasma on osteogenesis in rabbit.
      Authors: Zhang CQ, Yuan T, Zeng BF
      Chin. Med. J., 2004;117(12):1853-5.
      Species: Rabbit
      Sample Type: Plasma
    21. Autologous fibrin glue with growth factors in reconstructive maxillofacial surgery.
      Authors: Thorn JJ, Sorensen H, Weis-Fogh U, Andersen M
      Int J Oral Maxillofac Surg, 2004;33(1):95-100.
      Species: Human
      Sample Type: Complex Sample Type
    22. Fibrinogen induces chemotactic activity in endothelial cells.
      Authors: Seeger FH, Blessing E, Gu L, Bornhold R, Denger S, Kreuzer J
      Acta Physiol. Scand., 2002;176(2):109-15.
      Species: Porcine
      Sample Type: Cell Culture Supernates
    23. Ovariectomy increases mitogens and platelet-induced proliferation of arterial smooth muscle.
      Authors: Bracamonte MP, Rud KS, Owen WG, Miller VM
      Am. J. Physiol. Heart Circ. Physiol., 2002;283(3):H853-60.
      Species: Porcine
      Sample Type: Cell Lysates
    24. Cyclic mechanical stretching modulates secretion pattern of growth factors in human tendon fibroblasts.
      Authors: Skutek M, van Griensven M, Zeichen J, Brauer N, Bosch U
      Eur. J. Appl. Physiol., 2001;86(1):48-52.
      Species: Human
      Sample Type: Cell Culture Supernates
    25. Quantification of growth factor levels using a simplified method of platelet-rich plasma gel preparation.
      Authors: Landesberg R, Roy M, Glickman RS
      J. Oral Maxillofac. Surg., 2000;58(3):297.
      Species: Human
      Sample Type: Plasma
    26. Angiogenic balance in human melanoma: expression of VEGF, bFGF, IL-8, PDGF and angiostatin in relation to vascular density of xenografts in vivo.
      Authors: Westphal JR, Van't Hullenaar R, Peek R, Willems RW, Crickard K, Crickard U, Askaa J, Clemmensen I, Ruiter DJ, De Waal RM
      Int. J. Cancer, 2000;86(6):768-76.
      Species: Human
      Sample Type: Cell Culture Supernates
    27. Growth factors and proinflammatory cytokines in the renal involvement of POEMS syndrome.
      Authors: Soubrier M, Sauron C, Souweine B, Larroche C, Wechsler B, Guillevin L, Piette JC, Rousset H, Deteix P
      Am. J. Kidney Dis., 1999;34(4):633-8.
      Species: Human
      Sample Type: Serum
    28. Platelet-derived growth factor (PDGF-AB) like immune reactivity in serum and in cerebral spinal fluid following experimental subarachnoid haemorrhage in dogs.
      Authors: Vieweg U, 49315, Schramm J, Urbach H
      Acta Neurochir (Wien), 1999;141(8):861-5.
      Species: Canine
      Sample Type: Serum
    29. Hypoxia-induced expression of VEGF is reversible in myocardial vascular smooth muscle cells.
      Authors: Gu JW, Adair TH
      Am. J. Physiol., 1997;273(2):H628-33.
      Species: Canine
      Sample Type: Cell Culture Supernates
    Expand to show all 29 Citations
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