Human phospho-Chk2 (T68) Antibody

R&D Systems | Catalog # AF1626

R&D Systems
Discontinued Product
AF1626 has been discontinued. View all Chk2 products.

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Chk2 Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing human Chk2 T68 site

Specificity

Detects human Chk2 when phosphorylated at T68.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human phospho-Chk2 (T68) Antibody

Detection of Human Phospho-Chk2 (T68) antibody by Western Blot.

Detection of Human Phospho-Chk2 (T68) by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 1 hour. PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human Phospho-Chk2 (T68) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1626) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-Chk2 (T68) was detected at approximately 64 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda-phosphatase (lambda-PPase) for 60 minutes. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Phospho-Chk2 (T68) antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC).

Phospho-Chk2 (T68) in HepG2 Human Cell Line.

Phopsho-Chk2 (T68) was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line treated with 1 µM camptothecin using Rabbit Anti-Human Phospho-Chk2 (T68) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1626) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei in treated cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human phospho-Chk2 (T68) Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line treated with camptothecin, immersion fixed HepG2 human hepatocellular carcinoma cell line treated with camptothecin, and immersion fixed equine peripheral blood mononuclear cells (PBMCs) treated with calcium ionomycin and PMA

Western Blot

0.5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line treated with camptothecin

Reviewed Applications

Read 2 reviews rated 4.5 using AF1626 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Chk2

ATM phosphorylates the Checkpoint kinase on T68 in response to genotoxic insults. Phosphorylation of T68 promotes the formation of Chk2 oligomers. Oligomerization is a critical event in the activation of the Chk2 kinase as it is required for phosphorylation of additional Chk2 residues that lead to full activation and signaling to downstream effectors.

Long Name

Checkpoint Kinase 2

Alternate Names

CDS1, CHEK2, HuCds1, LFS2, PP1425, Rad53

Entrez Gene IDs

11200 (Human); 50883 (Mouse); 114212 (Rat)

Gene Symbol

CHEK2

Additional Chk2 Products

Product Documents for Human phospho-Chk2 (T68) Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human phospho-Chk2 (T68) Antibody

For research use only

Citations for Human phospho-Chk2 (T68) Antibody

Customer Reviews for Human phospho-Chk2 (T68) Antibody (2)

4.5 out of 5
2 Customer Ratings
5 Stars
50%
4 Stars
50%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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  • Human Phospho-Chk2 (T68) Antibody
    Name: Gabriele Strusi
    Application: Western Blot
    Sample Tested: HepG2 human hepatocellular carcinoma cell line
    Species: Human
    Verified Customer | Posted 01/17/2023
    Ab concentration 0.5 ug/mL
    Human phospho-Chk2 (T68) Antibody AF1626
  • Human Phospho-Chk2 (T68) Antibody
    Name: Isabelle Cristine da Costa
    Application: Western Blot
    Sample Tested: U2OS human osteosarcoma cell line
    Species: Human
    Verified Customer | Posted 09/23/2021
    Excellent antibody, it worked extremely well for western blotting.
    Human phospho-Chk2 (T68) Antibody AF1626

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