Human Phospho-Chk2 (T68) Antibody

Catalog # Availability Size / Price Qty
Phospho-Chk2 (T68) in HepG2 Human Cell Line.
2 Images
Product Details
Citations (7)
Supplemental Products
Reviews (1)

Human Phospho-Chk2 (T68) Antibody Summary

Species Reactivity
Detects human Chk2 when phosphorylated at T68.
Polyclonal Rabbit IgG
Antigen Affinity-purified
Phosphopeptide containing human Chk2 T68 site
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.5 µg/mL
See below
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry Phospho-Chk2 (T68) antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC). View Larger

Phospho-Chk2 (T68) in HepG2 Human Cell Line. Phopsho-Chk2 (T68) was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line treated with 1 µM camptothecin using Rabbit Anti-Human Phospho-Chk2 (T68) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1626) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei in treated cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Western Blot Detection of Human Phospho-Chk2 (T68) antibody by Western Blot. View Larger

Detection of Human Phospho-Chk2 (T68) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 1 hour. PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human Phospho-Chk2 (T68) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1626) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-Chk2 (T68) was detected at approximately 64 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda-phosphatase (lambda-PPase) for 60 minutes. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chk2

ATM phosphorylates the Checkpoint kinase on T68 in response to genotoxic insults. Phosphorylation of T68 promotes the formation of Chk2 oligomers. Oligomerization is a critical event in the activation of the Chk2 kinase as it is required for phosphorylation of additional Chk2 residues that lead to full activation and signaling to downstream effectors.

Long Name
Checkpoint Kinase 2
Entrez Gene IDs
11200 (Human); 50883 (Mouse); 114212 (Rat)
Alternate Names
CDS1; CHEK2; CHK2 checkpoint homolog (S. pombe); Chk2; EC 2.7.11; EC; HuCds1; LFS2; PP1425; Rad53; S.pombe) homolog

Product Datasheets

Citations for Human Phospho-Chk2 (T68) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
Filter your results:

Filter by:

  1. WRN helicase is a synthetic lethal target in microsatellite unstable cancers
    Authors: EM Chan, T Shibue, JM McFarland, B Gaeta, M Ghandi, N Dumont, A Gonzalez, JS McPartlan, T Li, Y Zhang, J Bin Liu, JB Lazaro, P Gu, CG Piett, A Apffel, SO Ali, R Deasy, P Keskula, RWS Ng, EA Roberts, E Reznichenk, L Leung, M Alimova, M Schenone, M Islam, YE Maruvka, Y Liu, J Roper, S Raghavan, M Giannakis, YY Tseng, ZD Nagel, A D'Andrea, DE Root, JS Boehm, G Getz, S Chang, TR Golub, A Tsherniak, F Vazquez, AJ Bass
    Nature, 2019;568(7753):551-556.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Starvation-induced activation of ATM/Chk2/p53 signaling sensitizes cancer cells to cisplatin.
    Authors: Shi Y, Felley-Bosco E, Marti T, Orlowski K, Pruschy M, Stahel R
    BMC Cancer, 2012;12(0):571.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. Multiple DNA damage signaling and repair pathways deregulated by simian virus 40 large T antigen.
    Authors: Boichuk S, Hu L, Hein J
    J. Virol., 2010;84(16):8007-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot
  4. RAD51C facilitates checkpoint signaling by promoting CHK2 phosphorylation.
    Authors: Badie S, Liao C, Thanasoula M, Barber P, Hill MA, Tarsounas M
    J. Cell Biol., 2009;185(4):587-600.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. Simian virus 40 large T antigen disrupts genome integrity and activates a DNA damage response via Bub1 binding.
    Authors: Hein J, Boichuk S, Wu J, Cheng Y, Freire R, Jat PS, Roberts TM, Gjoerup OV
    J. Virol., 2009;83(1):117-27.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot
  6. RNF8-dependent and RNF8-independent regulation of 53BP1 in response to DNA damage.
    Authors: Sakasai R, Tibbetts R
    J. Biol. Chem., 2008;283(20):13549-55.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Sonic Hedgehog signaling impairs ionizing radiation-induced checkpoint activation and induces genomic instability.
    Authors: Leonard JM, Ye H, Wetmore C, Karnitz LM
    J. Cell Biol., 2008;183(3):385-91.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Cell and Tissue Staining Kits

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Phospho-Chk2 (T68) Antibody

Average Rating: 5 (Based on 1 Review)

5 Star
4 Star
3 Star
2 Star
1 Star

Have you used Human Phospho-Chk2 (T68) Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:

Human Phospho-Chk2 (T68) Antibody
By Anonymous on 09/23/2021
Application: WB Sample Tested: U2OS human osteosarcoma cell line Species: Human

Excellent antibody, it worked extremely well for western blotting.