Human Phospho-Chk2 (T68) Antibody

Catalog # Availability Size / Price Qty
AF1626
AF1626-SP
Detection of Human Phospho-Chk2 (T68) by Western Blot.
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Product Details
Citations (6)
FAQs
Supplemental Products
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Human Phospho-Chk2 (T68) Antibody Summary

Species Reactivity
Human
Specificity
Detects human Chk2 when phosphorylated at T68.
Source
Polyclonal Rabbit IgG
Purification
Antigen Affinity-purified
Immunogen
Phosphopeptide containing human Chk2 T68 site
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Phospho-Chk2 (T68) by Western Blot. View Larger

Detection of Human Phospho-Chk2 (T68) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 µM camptothecin (CPT) for 1 hour. PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human Phospho‑Chk2 (T68) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1626) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-Chk2 (T68) was detected at approximately 64 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda -phosphatase ( lambda -PPase) for  60 minutes. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry Phospho-Chk2 (T68) in HepG2 Human Cell Line. View Larger

Phospho-Chk2 (T68) in HepG2 Human Cell Line. Phopsho-Chk2 (T68) was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line treated with 1 µM camptothecin using Rabbit Anti-Human Phospho-Chk2 (T68) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1626) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei in treated cells. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chk2

ATM phosphorylates the Checkpoint kinase on T68 in response to genotoxic insults. Phosphorylation of T68 promotes the formation of Chk2 oligomers. Oligomerization is a critical event in the activation of the Chk2 kinase as it is required for phosphorylation of additional Chk2 residues that lead to full activation and signaling to downstream effectors.

Long Name
Checkpoint Kinase 2
Entrez Gene IDs
11200 (Human); 50883 (Mouse); 114212 (Rat)
Alternate Names
CDS1; CHEK2; CHK2 checkpoint homolog (S. pombe); Chk2; EC 2.7.11; EC 2.7.11.1; HuCds1; LFS2; PP1425; Rad53; S.pombe) homolog

Product Datasheets

Citations for Human Phospho-Chk2 (T68) Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Starvation-induced activation of ATM/Chk2/p53 signaling sensitizes cancer cells to cisplatin.
    Authors: Shi Y, Felley-Bosco E, Marti T, Orlowski K, Pruschy M, Stahel R
    BMC Cancer, 2012;12(0):571.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  2. Multiple DNA damage signaling and repair pathways deregulated by simian virus 40 large T antigen.
    Authors: Boichuk S, Hu L, Hein J
    J. Virol., 2010;84(16):8007-20.
    Species: Human
    Sample Types: Whole Cells
    Applications: WB
  3. RAD51C facilitates checkpoint signaling by promoting CHK2 phosphorylation.
    Authors: Badie S, Liao C, Thanasoula M, Barber P, Hill MA, Tarsounas M
    J. Cell Biol., 2009;185(4):587-600.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  4. Simian virus 40 large T antigen disrupts genome integrity and activates a DNA damage response via Bub1 binding.
    Authors: Hein J, Boichuk S, Wu J, Cheng Y, Freire R, Jat PS, Roberts TM, Gjoerup OV
    J. Virol., 2009;83(1):117-27.
    Species: Human
    Sample Types: Whole Cells
    Applications: WB
  5. RNF8-dependent and RNF8-independent regulation of 53BP1 in response to DNA damage.
    Authors: Sakasai R, Tibbetts R
    J. Biol. Chem., 2008;283(20):13549-55.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB
  6. Sonic Hedgehog signaling impairs ionizing radiation-induced checkpoint activation and induces genomic instability.
    Authors: Leonard JM, Ye H, Wetmore C, Karnitz LM
    J. Cell Biol., 2008;183(3):385-91.
    Species: Human
    Sample Types: Cell Lysates
    Applications: WB

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Cell and Tissue Staining Kits

Isotype Controls

Normal Rabbit IgG Control

Ctrl,CyTOF-ready

Reconstitution Buffers

Secondary Antibodies

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