Human Phospho-IkB‑ alpha (S32/S36) Antibody

Name: Human Phospho-IkB‑ alpha (S32/S36) Antibody
Brand: R&D Systems
SKU: AF4809

R&D Systems | Catalog # AF4809

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Citations (4)

Product Documents

Discontinued Product

AF4809 has been discontinued. View all IkB-alpha products.

Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot, Multiplex Assay

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all IkB-alpha Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing human IkB-alpha S32/36 sites

Specificity

Detects human IkB-alpha when phosphorylated at S32/36.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Phospho-IkB‑ alpha (S32/S36) Antibody

Detection of Human Phospho-IkB‑ alpha (S32/S36) by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 20 ng/mL Recombinant Human TNF-a (Catalog # 210-TA) for 5 minutes. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human Phospho-IkB-a (S32/S36) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4809) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-IkB-a (S32/S36) was detected at approximately 44 kDa (as indicated). For additional reference, duplicate lysates were probed with 0.1 µg/mL Human IkB-a Monoclonal Antibody (lower panel, Catalog # MAB4299). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Phospho-IkB‑ alpha (S32/S36) Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line treated with Recombinant Human TNF‑ alpha (Catalog # 210-TA)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IkB-alpha

NF kappa B inhibitor alpha (IkB‑ alpha ) inhibits the NF kappa B complex by binding and sequestering it in the cytoplasm. Upon stimulation, IkB‑ alpha is phosphorylated on serine residues 32 and 36 marking it for degradation by the ubiquitin pathway. Following IkB‑ alpha degradation, the NF kappa B complex is able to translocate to the nucleus and activate transcription.