Human IkB-alpha Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human IkB-alpha in Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 417208
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human IkB-alpha
    Met1-Leu317
    Accession # P25963
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    See below
  • Simple Western
    1 µg/mL
    See below
  • Immunohistochemistry
    5-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human IkB-alpha by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, MCF‑7 human breast cancer cell line, PC‑3 human prostate cancer cell line, and LNCaP human prostate cancer cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human IkB-alpha Monoclonal Antibody (Catalog # MAB4299) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IkB-alpha at approximately 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Detection of Human IkB‑ alpha by Simple WesternTM. Simple Western lane view shows lysates of LNCaP human prostate cancer cell line and MCF‑7 human breast cancer cell line, loaded at 0.5 mg/mL. A specific band was detected for IkB‑ alpha at approximately 44 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human IkB‑ alpha Monoclonal Antibody (Catalog # MAB4299). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Immunohistochemistry
IkB‑ alpha in Human Prostate Cancer Tissue. IkB‑ alpha was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-Human IkB‑ alpha Monoclonal Antibody (Catalog # MAB4299) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IkB-alpha
NF kappa B inhibitor alpha (IkB-alpha ) inhibits the NF kappa B complex by binding and sequestering it in the cytoplasm. Upon stimulation, IkB‑ alpha is phosphorylated on serine residues marking it for degradation by the ubiquitin pathway. Following IkB‑ alpha degradation, the NF kappa B complex is able to translocate to the nucleus and activate transcription.
  • Long Name:
    I-kappa-B-alpha
  • Entrez Gene IDs:
    4792 (Human); 18035 (Mouse); 25493 (Rat)
  • Alternate Names:
    alpha; I-kappa-B-alpha; IkappaBalphaNF-kappa-B inhibitor alpha; IKBAikB-alpha; IkBalpha; IkB-alpha; MAD3; MAD-3; Major histocompatibility complex enhancer-binding protein MAD3; NFKBI; NFKBIA; nuclear factor of kappa light chain gene enhancer in B-cells; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. ERK5 protein promotes, whereas MEK1 protein differentially regulates, the Toll-like receptor 2 protein-dependent activation of human endothelial cells and monocytes.
    Authors: Wilhelmsen K, Mesa K, Lucero J, Xu F, Hellman J
    J Biol Chem, 2012;287(32):26478-94.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
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