|Detection of Human IkB-alpha by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, MCF‑7 human breast cancer cell line, PC‑3 human prostate cancer cell line, and LNCaP human prostate cancer cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human IkB-alpha Monoclonal Antibody (Catalog # MAB4299) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IkB-alpha at approximately 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.|
|Detection of Human IkB‑ alpha by Simple WesternTM. Simple Western lane view shows lysates of LNCaP human prostate cancer cell line and MCF‑7 human breast cancer cell line, loaded at 0.5 mg/mL. A specific band was detected for IkB‑ alpha at approximately 44 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human IkB‑ alpha Monoclonal Antibody (Catalog # MAB4299). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.|
|IkB‑ alpha in Human Prostate Cancer Tissue. IkB‑ alpha was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-Human IkB‑ alpha Monoclonal Antibody (Catalog # MAB4299) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.|
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