Human IkB-alpha Antibody

Catalog # Availability Size / Price Qty
MAB4299
MAB4299-SP

Save Up to 40% on RUO Reagents with BIOSPRING24 (See Details)

Detection of Human IkB-alpha  by Western Blot.
4 Images
Product Details
Citations (3)
FAQs
Supplemental Products
Reviews

Human IkB-alpha Antibody Summary

Species Reactivity
Human
Specificity
Detects human IkB-alpha in Western blots.
Source
Monoclonal Mouse IgG1 Clone # 417208
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IkB-alpha
Met1-Leu317
Accession # P25963
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Simple Western
1 µg/mL
See below
Immunohistochemistry
5-25 µg/mL
See below
Knockout Validated
IkB‑ alpha is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in IkB‑ alpha knockout HEK293T cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human IkB-a antibody by Western Blot. View Larger

Detection of Human IkB-alpha by Western Blot. Western blot shows lysates of Raji human Burkitt's lymphoma cell line, MCF-7 human breast cancer cell line, PC-3 human prostate cancer cell line, and LNCaP human prostate cancer cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human IkB-a Monoclonal Antibody (Catalog # MAB4299) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IkB-a at approximately 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.

Immunohistochemistry IkB-a antibody in Human Prostate Cancer Tissue by Immunohistochemistry (IHC-P). View Larger

IkB‑ alpha in Human Prostate Cancer Tissue. IkB-a was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Mouse Anti-Human IkB-a Monoclonal Antibody (Catalog # MAB4299) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei in cancer cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Simple Western Detection of Human IkB-a antibody by Simple Western<SUP>TM</SUP>. View Larger

Detection of Human IkB‑ alpha by Simple WesternTM. Simple Western lane view shows lysates of LNCaP human prostate cancer cell line and MCF‑7 human breast cancer cell line, loaded at 0.5 mg/mL. A specific band was detected for IkB‑ alpha at approximately 44 kDa (as indicated) using 1 µg/mL of Mouse Anti-Human IkB‑ alpha Monoclonal Antibody (Catalog # MAB4299). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Knockout Validated Western Blot Shows Human IkB-a Antibody Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human IkB‑ alpha Specificity by Using Knockout Cell Line. Western blot shows lysates of HEK293T human embryonic kidney parental cell line and IkB-a knockout HEK293T cell line (KO). PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human IkB-a Monoclonal Antibody (Catalog # MAB4299) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IkB-a at approximately 38 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IkB-alpha

NF kappa B inhibitor alpha (IkB-alpha ) inhibits the NF kappa B complex by binding and sequestering it in the cytoplasm. Upon stimulation, IkB‑ alpha is phosphorylated on serine residues marking it for degradation by the ubiquitin pathway. Following IkB‑ alpha degradation, the NF kappa B complex is able to translocate to the nucleus and activate transcription.

Long Name
I-kappa-B-alpha
Entrez Gene IDs
4792 (Human); 18035 (Mouse); 25493 (Rat)
Alternate Names
alpha; I-kappa-B-alpha; IkappaBalphaNF-kappa-B inhibitor alpha; IKBAikB-alpha; IkBalpha; IkB-alpha; MAD3; MAD-3; Major histocompatibility complex enhancer-binding protein MAD3; NFKBI; NFKBIA; nuclear factor of kappa light chain gene enhancer in B-cells; nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor

Product Datasheets

You must select a language.

x

Citations for Human IkB-alpha Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

  1. Interleukin-1&alpha Is a Critical Mediator of the Response of Human Bronchial Fibroblasts to Eosinophilic Inflammation
    Authors: K Bernau, JP Leet, H Floerke, EM Bruhn, AL Noll, IS McDermott, S Esnault, NN Jarjour, N Sandbo
    Cells, 2021-03-02;10(3):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Targeting Toll-like receptors with soluble Toll-like receptor 2 prevents peritoneal dialysis solution-induced fibrosis.
    Authors: Raby A, Gonzalez-Mateo G, Williams A, Topley N, Fraser D, Lopez-Cabrera M, Labeta M
    Kidney Int, 2018-05-31;94(2):346-362.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. ERK5 protein promotes, whereas MEK1 protein differentially regulates, the Toll-like receptor 2 protein-dependent activation of human endothelial cells and monocytes.
    Authors: Wilhelmsen K, Mesa K, Lucero J, Xu F, Hellman J
    J Biol Chem, 2012-06-15;287(32):26478-94.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human IkB-alpha Antibody

There are currently no reviews for this product. Be the first to review Human IkB-alpha Antibody and earn rewards!

Have you used Human IkB-alpha Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review