Human Phospho-IRS1 (Y612) Alexa Fluor™ Plus 555‑conjugated Antibody

Name: Human Phospho-IRS1 (Y612) Alexa Fluor™ Plus 555‑conjugated Antibody
Brand: R&D Systems
SKU: FAB7314AFP555
Price: 559 USD
Availability: InStock

R&D Systems | Catalog # FAB7314AFP555

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Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Alexa Fluor Plus 555 (Excitation = 558 nm, Emission = 572 nm)

Antibody Source

Monoclonal Mouse IgG_2A Clone # 738662

View all IRS1 Primary Antibodies »

Product Specifications

Specificity

Detects human IRS1 when phosphorylated at Y612 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2A

Applications for Human Phospho-IRS1 (Y612) Alexa Fluor™ Plus 555‑conjugated Antibody

Application

Recommended Usage

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Formulation

Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: IRS1

Human IRS1 (insulin receptor substrate 1) is a 160-180 kDa substrate that transmits signals from the insulin and IGF-I receptor to downstream signaling modulators. The 1242 amino acid (aa) human IRS1 contains a PH domain (aa 12-115), a PTB domain (aa 160-263) and ten PEST sequences (aa 340-1225). IRS1 may be proteolytically cleaved at Arg656-Val657, generating a 90 kDa and 79 kDa fragment. Upon insulin/IGF-I receptor activation, IRS1 is tyrosine phosphorylated, allowing its association with PI-3 kinase and GRB2. Phosphorylation on Y612 and Y632 is important for IRS1 to fully activate PI3K and translocate GLUT4 in respose to insulin. The sequence surrounding pTyr612 is identical between human and mouse IRS1.

Long Name

Insulin Receptor Substrate 1

Alternate Names

HIRS-1, insulin receptor substrate 1, IRS-1

Entrez Gene IDs

3667 (Human); 16367 (Mouse); 25467 (Rat)

Gene Symbol

IRS1

UniProt

P35568

Additional IRS1 Products

Product Documents for Human Phospho-IRS1 (Y612) Alexa Fluor™ Plus 555‑conjugated Antibody

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Certificate of Analysis

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Product Specific Notices for Human Phospho-IRS1 (Y612) Alexa Fluor™ Plus 555‑conjugated Antibody

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

For research use only