Human Phospho-MEK1 (T386) Antibody

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Detection of Phospho-MEK1 (T386) by Western Blot
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Product Details
Supplemental Products

Human Phospho-MEK1 (T386) Antibody Summary

Species Reactivity
Human ~45 kDa MEK1 phosphorylated at T386
Polyclonal Rabbit IgG
Antigen Affinity-purified
Phosphopeptide corresponding to amino acid residues surrounding the human phospho-T386 of MEK1
100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.


Recommended Concentration
Western Blot
1:1000 dilution
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Phospho-MEK1 (T386) antibody by Western Blot View Larger

Detection of Phospho-MEK1 (T386) by Western Blot Western blot of human T47D cells showing specific immunolabeling of the ~45 kDa MEK1 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda phosphatase, lambda PPase). The blot is identical to the control except that it was incubated in lambda PPase (1200 units for 30 minutes) before being exposed to the MEK1 (T386) antibody. The immunolabeling of MEK1 is completely eliminated by lambda PPase.

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: MEK1

Human MEK1 (MAPK/ERK kinase 1) is a 45 kDa member of the MAP kinase kinase subfamily of the Ser/Thr protein kinase family of enzymes. It is 393 amino acids (aa) in length and contains one ERK interaction (aa 1 - 32) and catalytic site (aa 68 - 361). There is one alternate splice form that shows a 26 aa deletion between aa 471 - 548. MEK1 is part of the MAP kinase pathway that is activated in response to growth factors and integrins. Two signal transduction pathways converge on MEK1. Mitogens activate Ras, which activates Raf, which activates MEK1. Integrins activate Rac, which activates PAK, which also activates MEK1. Raf activates MEK1 by phosphorylating S218 and S222 on MEK1. Alternatively, PAK indirectly activates MEK1 by phosphorylating MEK1 on S298, which promotes the Raf phosphorylation of S218 and S222. Once activated, MEK1 phosphorylates and activates ERK1/2. In a negative feedback loop, activated ERK can phosphorylate MEK1 on T292 and T386. This phosphorylation of T292 will interfere with PAK phosphorylation of MEK1 on S298. ERK-mediated phosphorylation of MEK1 at T386 may also interfere with PAK phosphorylation of MEK1.

  1. Chong, H. et al. (2003) Cell. Signal. 15:463.
  2. Eblen, S.T. et al. (2004) Mol. Cell. Biol. 24:2308.
  3. Xu, B. et al. (1999) J. Biol. Chem. 274:34029.
  4. Frost, J.A. et al. (1997) EMBO J. 16:6426.
  5. Mansour, S.J. et al. (1994) J. Biochem. 116:304.
  6. Zheng, C-F. and K-L. Guan (1994) EMBO J. 13:1123.
Long Name
Mitogen-activated Protein Kinase Kinase 1
Entrez Gene IDs
5604 (Human); 26395 (Mouse)
Alternate Names
dual specificity mitogen-activated protein kinase kinase 1; ERK activator kinase 1; MAP kinase kinase 1; MAP2K1; MAPK/ERK kinase 1; MAPKK1; MEK1; MEK1MAPKK 1; mitogen-activated protein kinase kinase 1; MKK1; MKK1MEK 1; PRKMK1EC; protein kinase, mitogen-activated, kinase 1 (MAP kinase kinase 1)

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