Human Phospho-MKK3/MKK6 (MKK3 S218/T222, MKK6 S207/T211) Antibody

Name: Human Phospho-MKK3/MKK6 (MKK3 S218/T222, MKK6 S207/T211) Antibody
Brand: R&D Systems
SKU: AF4930

Catalog #: AF4930 Datasheet / COA / SDS

Discontinued Product

AF4930 has been discontinued.

View all MKK3/MKK6 products.

Detection of Human Phospho-MKK3/ MKK6 (MKK3 S218/T222, MKK6 S207/T211) by Western Blot.

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Human Phospho-MKK3/MKK6 (MKK3 S218/T222, MKK6 S207/T211) Antibody Summary

Species Reactivity

Human

Specificity

Detects human Phospho-MKK3/MKK6 (MKK3 S218/T222, MKK6 S207/T211) in Western blots.

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Phosphopeptide containing human MKK6 S207/T211 site

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Phospho-MKK3/ MKK6 (MKK3 S218/T222, MKK6 S207/T211) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or exposed (+) to 100 J/m²of UV-C for 30 minutes and HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 20 ng/mL Recombinant Human IL-1 beta /IL-1F2 (Catalog # 201-LB) for 20 minutes. PVDF membrane was probed with 5 µg/mL Rabbit Anti-Human Phospho-MKK3/MKK6 (MKK3 S218/T222, MKK6 S207/T211) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4930) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands for Phospho-MKK3/MKK6 (MKK3 S218/T222, MKK6 S207/T211) were detected at approximately 40 and 38 kDa (as indicated). For additional reference, duplicate lysates were probed with 1 µg/mL Human/Mouse/Rat MKK3/MKK6 Monoclonal Antibody (lower panel, Catalog # MAB2514). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: MKK3/MKK6

Mitogen-activated protein kinase kinase 3 (MKK3) and the closely related MKK6 are activated by proinflammatory cytokines and environmental stresses. Activation occurs through dual phosphorylation at S218/T222 of MKK3 and S207/T211 of MKK6 by several upstream MAP3Ks, including ASK1 and TAK1. Both MKK3 and MKK6 are dual-specificity protein kinases, phosphorylating and activating the p38 MAP kinases at T and Y positions within the phosphoacceptor sequence T-G-Y.

Long Name

Mitogen-activated Protein Kinase Kinase 3

Alternate Names

Dual specificity mitogen-activated protein kinase kinase 6;MAP2K6;MAPKK6;MEK6;MKK6;PRKMK6;SAPKK3;SAPKK-3; MKK3/MKK6