Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot, Immunocytochemistry, Chromatin Immunoprecipitation (ChIP)

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1074A
View all Smad2/3 Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing the human Smad2 S465/S467 site
Accession # NP_005892

Specificity

Detects human Smad2 and Smad3 when dually phosphorylated at S465 and S467 in Western blots.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Phospho-Smad2/3 (S465/S467) Antibody

Detection of Human Phospho-Smad2/3 (S465/S467) antibody by Western Blot.

Detection of Human Phospho-Smad2/3 (S465/S467) by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 10 ng/mL Recombinant Human TGF-beta 1 (Catalog # 240-B) for 30 minutes. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human Phospho-Smad2/3 (S465/S467) Monoclonal Antibody (Catalog # MAB8935) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Smad2/3 (S465/S467) at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Detection of Phospho-Smad2/3 (S465/S467) by Western Blot

Detection of Phospho-Smad2/3 (S465/S467) by Western Blot

PAO-induced production of IL-33 in primary human airway epithelial cells. BEAS-2B cells were treated with PAO (0, 20, 50, 100 nM) for 24 h. (a, b) Immunoblot and quantitative analysis for the expression of IL-33. (c) Representative immunoblot (d) quantitative analysis for the expression of pSMAD2/3, SMAD2/3, and GAPDH. Experiments were conducted in triplicate. Data are presented as mean ± SEM, *P < 0.05, ANOVA, as compared control (vehicle) to PAO-treated cells. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40195488), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Phospho-Smad2/3 (S465/S467) Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line treated with Recombinant Human TGF‑ beta 1 (Catalog # 240-B)

Reviewed Applications

Read 2 reviews rated 4.5 using MAB8935 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Smad2/3

Smads are a family of intracellular proteins that transmit transforming growth factor beta (TGF-beta ) superfamily signals from the cell surface to the nucleus. The Smad family is divided into three subclasses: receptor regulated Smads, (Smads 1, 2, 3, 5 and 8); the common partner, (Smad4); and the inhibitory Smads, (Smads 6 and 7). The binding of TGF-beta or activin to their cognate receptor induces phosphorylation of Smads 2 and 3. The activated Smads associate with the common-mediator subunit, Smad4, and the heteromeric complex translocates into the nucleus to initiate transcription. Smad3, also known as Mothers Against Decapentaplegic homolog 3 (MADH3), shares 83% amino acid identity with Smad2, also known as Mothers Against Decapentaplegic homolog 2 (MADH2). Human Smad2 has 99% identity to mouse and rat Smad2. Human Smad3 has 99% identity to mouse and rat Smad3.

Long Name

Mothers Against DPP Homolog 2/3

Alternate Names

hMAD-2, hSMAD2, JV18, JV18-1SMAD, mothers against DPP homolog 2 (Drosophila), MAD homolog 2, Mad protein homolog, Mad, mothers against decapentaplegic homolog 2, MADH2MGC34440, MADR2MGC22139, Mad-related protein 2, mother against DPP homolog 2, mothers against decapentaplegic homolog 2, Mothers against DPP homolog 2, Sma- and Mad-related protein 2, SMAD 2, SMAD family member 2MAD, mothers against decapentaplegic homolog 2 (Drosophila), SMAD, mothers against DPP homolog 2, Smad2

UniProt

NP_005892

Additional Smad2/3 Products

Product Documents for Human Phospho-Smad2/3 (S465/S467) Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Phospho-Smad2/3 (S465/S467) Antibody

For research use only

Citations for Human Phospho-Smad2/3 (S465/S467) Antibody

Customer Reviews for Human Phospho-Smad2/3 (S465/S467) Antibody (2)

4.5 out of 5
2 Customer Ratings
5 Stars
50%
4 Stars
50%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human Phospho-Smad2/3 (S465/S467) Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 2 of 2 reviews Showing All
Filter By:
Clear Filters X
  • Human Phospho-Smad2/3 (S465/S467) Antibody
    Name: Pauline Jeannot
    Application: Western Blot
    Sample Tested: primary astrocytes
    Species: Human
    Verified Customer | Posted 08/21/2024
    Western blot using lysates from human primary astrocytes 15min after stimulation with human TGF-beta. 120ug of protein have been loaded.
    Lysates from primary human astrocytes
    Human Phospho-Smad2/3 (S465/S467) Antibody MAB8935
  • Human Phospho-Smad2/3 (S465/S467) Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Mv1Lu mink lung epithelial cell line
    Species: Mink
    Verified Customer | Posted 07/08/2020
    Human Phospho-Smad2/3 (S465/S467) Antibody MAB8935

There are no reviews that match your criteria.

Showing  1 - 2 of 2 reviews Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies