Human Phospho-STAT6 (Y641) Antibody

Name: Human Phospho-STAT6 (Y641) Antibody
Brand: R&D Systems
SKU: AF3717

R&D Systems | Catalog # AF3717

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Citations (2)

Product Documents

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Discontinued Product

AF3717 has been discontinued. An alternative/replacement product is available: MAB3717. View all STAT6 products.

Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Intracellular Staining by Flow Cytometry, Simple Western

Cited:

Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all STAT6 Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing human STAT6 Y641 site.

Specificity

Detects human STAT6 when phosphorylated at Y641.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Phospho-STAT6 (Y641) Antibody

Detection of STAT6 in Daudi Human Cell Line by Flow Cytometry.

Daudi human Burkitt's lymphoma cell line was unstimulated (light orange filled histogram) or treated with 100 ng/mL Recombinant Human IL-4 (Catalog # 204-IL) for 20 minutes, then stained with Rabbit Anti-Human Phospho-STAT6 (Y641) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3717, dark orange filled histogram) or isotype control antibody (Catalog # AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with methanol.

Detection of Human Phospho-STAT6 (Y641) by Western Blot.

Western blot shows lysates of U937 human histiocytic lymphoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IL-4 (Catalog # 204-IL) for 30 minutes. Gels were loaded with 20 µg of cytoplasmic (Cyto) and 10 µg of nuclear extracts (Nuc). PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human Phospho-STAT6 (Y641) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3717) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-STAT6 (Y641) was detected at approximately 110 kDa (as indicated). For additional reference, the membrane was stripped and reprobed with 0.5 µg/mL Human STAT6 Monoclonal Antibody (lower panel, Catalog # MAB2167). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Phospho-STAT6 (Y641) by Simple Western^TM.

Simple Western lane view shows lysates of U937 human histiocytic lymphoma cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human IL-4 (Catalog # 204-IL) for 30 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-STAT6 (Y641) at approximately 101 kDa (as indicated) using 5 µg/mL of Rabbit Anti-Human Phospho-STAT6 (Y641) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3717). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Applications for Human Phospho-STAT6 (Y641) Antibody

Application

Recommended Usage

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells
Sample: Daudi human Burkitt's lymphoma cell line treated with Recombinant Human IL‑4 (Catalog # 204-IL), fixed with paraformaldehyde, and permeabilized with methanol

Simple Western

5 µg/mL
Sample: U937 human histiocytic lymphoma cell line treated with Recombinant Human IL‑4 (Catalog # 204-IL)

Western Blot

0.5 µg/mL
Sample: U937 human histiocytic lymphoma cell line treated with Recombinant Human IL‑4 (Catalog # 204-IL)

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

Build Your Panel Now

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

For liquid material, refer to CoA for concentration.

Formulation

Supplied as a 0.2 μm filtered solution in PBS. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C, as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
6 months, -20 to -70 °C under sterile conditions after opening.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: STAT6

Signal Transducer and Activator of Transcription 6 (STAT6) mediates the signaling of cytokines such as IL-4 and IL-13. STAT6 acts as a signal transducer in the cytoplasm and, upon phosphorylation at Y641, translocates to the nucleus and binds to the DNA consensus site TTCN₄GAA. Knockout studies in mice suggest that STAT6 functions in differentiation of T helper 2 (Th2) cells, expression of cell surface markers, and class switch of immunoglobulins.

Long Name

Signal Transducer and Activator of Transcription 6

Alternate Names

D12S1644, EC 2.4.1.227, EC 2.7.7.6, IL-4 Stat, IL-4-STAT, signal transducer and activator of transcription 6, signal transducer and activator of transcription 6, interleukin-4 induced, STAT, interleukin-4 induced, STAT, interleukin4-induced, STAT6B, STAT6C, transcription factor IL-4 STAT