Human STAT6 Antibody Summary
Accession # P42226.1
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
STAT6 in Human PBMCs. STAT6 was detected in immersion fixed PHA-stimulated human peripheral blood mononuclear cells (PBMCs) using 8 µg/mL Mouse Anti-Human STAT6 Monoclonal Antibody (Catalog # MAB2167) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Detection of Human STAT6 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human STAT6 Monoclonal Antibody (Catalog # MAB2167) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for STAT6 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
STAT family proteins mediate cytokine signaling by acting as signal transducers in the cytoplasm and transcription activators in the nucleus. STAT6 is important for the development of Th2 cells and regulation of gene expression induced by IL-4 and IL-13.
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