Human STAT6 Antibody

Catalog # Availability Size / Price Qty
MAB2167
MAB2167-SP
Human STAT6 Antibody in Immunocytochemistry
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Human STAT6 Antibody Summary

Species Reactivity
Human
Specificity
Detects human, mouse, and rat STAT6.
Source
Monoclonal Mouse IgG2A Clone # 253906
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human STAT6
Asn341-Gly640
Accession # P42226.1
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Jurkat human acute T cell leukemia cell line fixed with paraformaldehyde and permeabilized with methanol

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry STAT6 in Human PBMCs. View Larger

STAT6 in Human PBMCs. STAT6 was detected in immersion fixed PHA-stimulated human peripheral blood mononuclear cells (PBMCs) using 8 µg/mL Mouse Anti-Human STAT6 Monoclonal Antibody (Catalog # MAB2167) for 3 hours at room temperature. Cells were stained (red) and counterstained (green). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Western Blot Detection of Human STAT6 by Western Blot. View Larger

Detection of Human STAT6 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human STAT6 Monoclonal Antibody (Catalog # MAB2167) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for STAT6 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 5.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: STAT6

STAT family proteins mediate cytokine signaling by acting as signal transducers in the cytoplasm and transcription activators in the nucleus. STAT6 is important for the development of Th2 cells and regulation of gene expression induced by IL-4 and IL-13.

Long Name
Signal Transducer and Activator of Transcription 6
Entrez Gene IDs
6778 (Human); 20852 (Mouse); 362896 (Rat)
Alternate Names
D12S1644; EC 2.4.1.227; EC 2.7.7.6; IL-4 Stat; IL-4-STAT; signal transducer and activator of transcription 6; signal transducer and activator of transcription 6, interleukin-4 induced; STAT, interleukin-4 induced; STAT, interleukin4-induced; STAT6; STAT6B; STAT6C; transcription factor IL-4 STAT

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Cell and Tissue Staining Kits

Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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