Human PKC delta Antibody

Catalog # Availability Size / Price Qty
AF4585
AF4585-SP
Human PKC delta Antibody in Western Blot
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Product Details
Citations (1)
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Human PKC delta Antibody Summary

Species Reactivity
Human
Specificity
Detects human PKCδ in Western blots.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human PKCδ
His549-Asp676
Accession # Q05655
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human PKCδ by Western Blot. View Larger

Detection of Human PKCδ by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line and HT-29 human colon adenocarcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human PKCd Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4585) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PKCd at approximately 78 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PKC delta

PKCδ (protein kinase C-delta) is a 78 kDa member of the PKC subfamily, AGC Ser/Thr protein kinase family of enzymes. It is a Ca++‑independent and phospholipid‑dependent ubiquitous enzyme that participates in cellular apoptosis. Human PKCδ is 676 amino acids (aa) in length. It contains two general regions: a phosphotyrosine and DAG-type 2 binding region (aa's 1‑90 and 159‑208, respectively), and an ATP-binding catalytic region (aa 349‑603). Functionally, inactive cytoplasmic PKCδ is first activated by Tyr64 and Tyr155 phosphorylation. This induces PKCδ entry into the nucleus, where it is cleaved into 43 kDa and 36 kDa fragments, initiating apoptosis. There are two potential alternate start sites for PKCδ at Met51 and Met74. In some cells, a poorly characterized 160 kDa form of PKCδ has been noted. Over aa 549‑676, human PKCδ is 85% aa identical to mouse PKCδ.

Long Name
Protein Kinase C delta
Entrez Gene IDs
5580 (Human)
Alternate Names
EC 2.7.11; EC 2.7.11.13; MAY1; MGC49908; nPKC-delta; PKC delta; PKCD; PKCy; PRKCD; protein kinase C delta type; protein kinase C delta VIII; protein kinase C, delta

Product Datasheets

Citation for Human PKC delta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. p63 and p73, members of the p53 gene family, transactivate PKCdelta.
    Authors: Ponassi, Raffaell, Terrinoni, Alessand, Chikh, Anissa, Rufini, Alessand, Lena, Anna Mar, Sayan, Berna S, Melino, Gerry, Candi, Eleonora
    Biochem Pharmacol, 2006;72(11):1417-22.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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