Human PKC delta Antibody

Name: Human PKC delta Antibody
Brand: R&D Systems
SKU: AF4585
Price: 159 USD
Availability: InStock

R&D Systems | Catalog # AF4585

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all PKC delta Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PKC delta
His549-Asp676
Accession # Q05655

Specificity

Detects human PKC delta in Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human PKC delta Antibody

Detection of Human PKC delta by Western Blot.

Western blot shows lysates of LNCaP human prostate cancer cell line and HT-29 human colon adenocarcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human PKCd Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4585) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PKCd at approximately 78 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for Human PKC delta Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: LNCaP human prostate cancer cell line and HT‑29 human colon adenocarcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: PKC delta

PKC delta (protein kinase C-delta) is a 78 kDa member of the PKC subfamily, AGC Ser/Thr protein kinase family of enzymes. It is a Ca⁺⁺‑independent and phospholipid‑dependent ubiquitous enzyme that participates in cellular apoptosis. Human PKC delta is 676 amino acids (aa) in length. It contains two general regions: a phosphotyrosine and DAG-type 2 binding region (aa's 1‑90 and 159‑208, respectively), and an ATP-binding catalytic region (aa 349‑603). Functionally, inactive cytoplasmic PKC delta is first activated by Tyr64 and Tyr155 phosphorylation. This induces PKC delta entry into the nucleus, where it is cleaved into 43 kDa and 36 kDa fragments, initiating apoptosis. There are two potential alternate start sites for PKC delta at Met51 and Met74. In some cells, a poorly characterized 160 kDa form of PKC delta has been noted. Over aa 549‑676, human PKC delta is 85% aa identical to mouse PKC delta.