Human PlGF Antibody [Biotin]

R&D Systems | Catalog # BAF264

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Detection (Matched Antibody Pair)

Cited:

ELISA Development, ELISA Development (Detection)

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all PlGF Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PlGF
Ala21-Arg149
Accession # CAA38698

Specificity

Detects human PlGF in ELISAs and Western blots. In sandwich immunoassays, less than 20% cross-reactivity with recombinant human (rh) VEGF/PlGF is observed and less than 0.07% cross-reactivity with rhFlt-1, recombinant mouse PlGF-2, rhPDGF, and rhVEGF is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human PlGF Antibody [Biotin]

Human PlGF ELISA Standard Curve

Human PlGF ELISA Standard Curve

Recombinant Human PlGF (Catalog # 264-PGB) was serially diluted and captured by Mouse Anti-Human PlGF Monoclonal Antibody (Catalog # MAB264) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human PlGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-264-PB) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human PlGF Antibody [Biotin]

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human PlGF (Catalog # 264-PGB)

Human PlGF Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Human PlGF Antibody (Catalog # MAB264)
  • Capture Reagent: Human PlGF Antibody (Catalog # MAB264R)
  • Standard: Recombinant Human PlGF Protein (Catalog # 264-PGB)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using BAF264 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PlGF

Placenta growth factor (PlGF) is a member of the vascular endothelial growth factor (VEGF) family of growth factors. PlGF and VEGF share primary structural as well as limited amino acid sequence homology with the A and B chains of PDGF. All eight cysteine residues involved in intra- and inter-chain disulfides are conserved among these growth factors. In their PDGF-like regions, VEGF and PlGF also share approximately 53% amino acid sequence similarity. The gene for PlGF has been mapped to chromosome 14. As a result of alternative splicing, at least two PlGF mRNAs encoding monomeric PlGF precursors containing 149 and 170 amino acid residues have been described. The expression of PlGF is not widespread, but has been detected in human umbilical vein endothelial cells, placenta, choriocarcinoma cell lines, and in renal cell carcinoma associated with angiogenesis. The PlGF proteins bind with high-affinity to Flt-1, but not to Flk-1/KDR.

Long Name

Placenta Growth Factor

Alternate Names

PGF, PGFL

Entrez Gene IDs

5228 (Human); 18654 (Mouse)

Gene Symbol

PGF

UniProt

CAA38698

Additional PlGF Products

Product Documents for Human PlGF Antibody [Biotin]

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PlGF Antibody [Biotin]

For research use only

Citations for Human PlGF Antibody [Biotin]

Customer Reviews for Human PlGF Antibody [Biotin] (1)

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  • Human PlGF Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: U87
    Species: Human
    Verified Customer | Posted 02/17/2021
    Quantification of the amount of PlGF in the conditioned medium was performed using anti-PlGF IgGs at a concentration of 10 μg/ml in PBS, to coat Maxisorp Nunc immunoplates
    Human PlGF Antibody [Biotin] BAF264

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