Human PlGF Antibody Summary
Accession # CAA38698
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human PlGF by Western Blot. Western blot shows lysates of CHO Chinese hamster ovary cell line either mock transfected or transfected with full length human PlGF. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human PlGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-264-PB) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PlGF at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Placenta Growth Factor (PlGF) is a member of the Vascular Endothelial Growth Factor (VEGF) family of growth factors. PlGF and VEGF share primary structural as well as limited amino acid sequence homology with the A and B chains of PDGF. All eight cysteine residues involved in intra- and inter-chain disulfides are conserved among these growth factors. In their PDGF-like regions, VEGF and PlGF also share approximately 53% amino acid sequence similarity. The gene for PlGF has been mapped to chromosome 14. As a result of alternative splicing, at least two PlGF mRNAs encoding monomeric PlGF precursors containing 149 and 170 amino acid residues have been described. The expression of PlGF is not widespread, but has been detected in human umbilical vein endothelial cells, placenta, choriocarcinoma cell lines, and in renal cell carcinoma associated with angiogenesis. The PlGF proteins bind with high-affinity to Flt-1, but not to Flk-1/KDR.
Citations for Human PlGF Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 4
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Placental growth factor neutralising antibodies give limited anti-angiogenic effects in an in vitro organotypic angiogenesis model.
Authors: Brave SR, Eberlein C, Shibuya M, Wedge SR, Barry ST
Sample Types: Whole Cells
VEGF-A and VEGF-F evoke distinct changes in vascular ultrastructure.
Authors: Matsunaga Y, Yamazaki Y, Suzuki H, Morita T
Biochem. Biophys. Res. Commun., 2009;379(4):872-5.
Species: Guinea Pig
Sample Types: Whole Tissue
Malignant mesothelioma growth inhibition by agents that target the VEGF and VEGF-C autocrine loops.
Authors: Masood R, Kundra A, Zhu S, Xia G, Scalia P, Smith DL, Gill PS
Int. J. Cancer, 2003;104(5):603-10.
Sample Types: Cell Lysates
Applications: Western Blot
Vascular endothelial growth factor ligands and receptors that regulate human cytotrophoblast survival are dysregulated in severe preeclampsia and hemolysis, elevated liver enzymes, and low platelets syndrome.
Authors: Zhou Y, 2019, McMaster M, Woo K, Janatpour M, Perry J, Karpanen T, Alitalo K, Damsky C, Fisher SJ
Sample Types: Whole Tissue
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