Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all PLRP1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human PLRP1
Lys18-Cys467
Accession # P54315

Specificity

Detects human PLRP1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human PLRP1 Antibody

Detection of Human PLRP1 antibody by Western Blot.

Detection of Human PLRP1 by Western Blot.

Western blot shows lysates of human pancreas tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human PLRP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7105) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PLRP1 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human PLRP1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human pancreas tissue

Reviewed Applications

Read 1 review rated 5 using AF7105 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PLRP1

PNLIPRP1 (pancreatic lipase related protein 1; also PLRP1) is a secreted 50-55 kDa member of the lipase family, AB hydrolase superfamily of proteins. It is expressed by sublingual gland epithelium (in mouse) and pancreatic acinar cells, and is now suggested to act as a negative regulator of pancreatic lipase (PL) activity by competing with PL for colipase occupancy. Mature human PLRP1 is 450 amino acids (aa) in length (aa 18-467). It contains one PLAT domain (aa 356-467), and a Val196/Ala198 motif that inactivates its otherwise expected intrinsic lipase activity. There are two isoform variants. One shows a 76 aa substitution for aa 111-467, while another shows a seven aa substitution for aa 111-467. Mature human PLRP1 (aa 18-467) shares 83% aa identity with mature mouse PLRP1.

Long Name

Pancreatic Lipase-related Protein 1

Alternate Names

PNLIPRP1

Entrez Gene IDs

5407 (Human); 18946 (Mouse); 84028 (Rat)

Gene Symbol

PNLIPRP1

UniProt

P54315 (Human)

Additional PLRP1 Products

Product Documents for Human PLRP1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PLRP1 Antibody

For research use only

Related Research Areas

Cancer Biomarkers

Customer Reviews for Human PLRP1 Antibody (1)

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1 Customer Rating
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  • Human PLRP1 Antibody
    Name: Doug Chan
    Application: Western Blot
    Sample Tested: Tumor cell lyastes
    Species: Human
    Verified Customer | Posted 06/17/2022
    Excellent antibody for western blot.
    Human PLRP1 Antibody AF7105

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