Human PLRP1 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human PLRP1 in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human PLRP1
    Lys18-Cys467
    Accession # P54315
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human PLRP1 by Western Blot. Western blot shows lysates of human pancreas tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human PLRP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7105) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PLRP1 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PLRP1

PNLIPRP1 (pancreatic lipase related protein 1; also PLRP1) is a secreted 50-55 kDa member of the lipase family, AB hydrolase superfamily of proteins. It is expressed by sublingual gland epithelium (in mouse) and pancreatic acinar cells, and is now suggested to act as a negative regulator of pancreatic lipase (PL) activity by competing with PL for colipase occupancy. Mature human PLRP1 is 450 amino acids (aa) in length (aa 18-467). It contains one PLAT domain (aa 356-467), and a Val196/Ala198 motif that inactivates its otherwise expected intrinsic lipase activity. There are two isoform variants. One shows a 76 aa substitution for aa 111-467, while another shows a seven aa substitution for aa 111-467. Mature human PLRP1 (aa 18-467) shares 83% aa identity with mature mouse PLRP1.

  • Long Name:
    Pancreatic Lipase-related Protein 1
  • Entrez Gene IDs:
    5407 (Human); 18946 (Mouse); 84028 (Rat)
  • Alternate Names:
    EC 3.1.1; EC 3.1.1.3; pancreatic lipase-related protein 1; PLRP1; PLRP1PL-RP1; PNLIPRP1
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 5
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Molecular Weight Markers
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Full-Range Molecular Weight Marker, Biotinylated

MW001
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG Horseradish Peroxidase-conjugated Antibody

WB, Simple Western HAF016 7  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 2
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 3
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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