Detection of Human PLRP1 by Western Blot.
Western blot shows lysates of human pancreas tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human PLRP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7105) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PLRP1 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
PNLIPRP1 (pancreatic lipase related protein 1; also PLRP1) is a secreted 50-55 kDa member of the lipase family, AB hydrolase superfamily of proteins. It is expressed by sublingual gland epithelium (in mouse) and pancreatic acinar cells, and is now suggested to act as a negative regulator of pancreatic lipase (PL) activity by competing with PL for colipase occupancy. Mature human PLRP1 is 450 amino acids (aa) in length (aa 18-467). It contains one PLAT domain (aa 356-467), and a Val196/Ala198 motif that inactivates its otherwise expected intrinsic lipase activity. There are two isoform variants. One shows a 76 aa substitution for aa 111-467, while another shows a seven aa substitution for aa 111-467. Mature human PLRP1 (aa 18-467) shares 83% aa identity with mature mouse PLRP1.
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