PNLIPRP1 (pancreatic lipase related protein 1; also PLRP1) is a secreted 50-55 kDa member of the lipase family, AB hydrolase superfamily of proteins. It is expressed by sublingual gland epithelium (in mouse) and pancreatic acinar cells, and is now suggested to act as a negative regulator of pancreatic lipase (PL) activity by competing with PL for colipase occupancy. Mature human PLRP1 is 450 amino acids (aa) in length (aa 18-467). It contains one PLAT domain (aa 356-467), and a Val196/Ala198 motif that inactivates its otherwise expected intrinsic lipase activity. There are two isoform variants. One shows a 76 aa substitution for aa 111-467, while another shows a seven aa substitution for aa 111-467. Mature human PLRP1 (aa 18-467) shares 83% aa identity with mature mouse PLRP1.
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Lys18-Cys467
Accession # P54315
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human PLRP1 Antibody
Detection of Human PLRP1 by Western Blot.
Western blot shows lysates of human pancreas tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human PLRP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7105) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PLRP1 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Applications for Human PLRP1 Antibody
Western Blot
Sample: Human pancreas tissue
Reviewed Applications
Read 1 review rated 5 using AF7105 in the following applications:
Formulation, Preparation, and Storage
Purification
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: PLRP1
Long Name
Alternate Names
Gene Symbol
UniProt
Additional PLRP1 Products
Product Documents for Human PLRP1 Antibody
Certificate of Analysis
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Product Specific Notices for Human PLRP1 Antibody
For research use only
Related Research Areas
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Application: Western BlotSample Tested: Tumor cell lyastesSpecies: HumanVerified Customer | Posted 06/17/2022Excellent antibody for western blot.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
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