Human Pro‑BDNF Antibody

R&D Systems | Catalog # MAB31751

R&D Systems
Discontinued Product
MAB31751 has been discontinued. View all BDNF products.

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Western Blot, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 584412
View all BDNF Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Pro-BDNF
Ala19-Arg247
Accession # P23560

Specificity

Detects human Pro-BDNF in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse Pro-BDNF is observed and no cross-reactivity with recombinant human  beta -NGF, NT-4, Pro-NT-3 or Pro-NT-4 is observed. In Western blots, 100% cross-reactivity with recombinant mouse Pro-BDNF is observed and no cross-reactivity with recombinant human mature BDNF or recombinant human NT-3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Pro‑BDNF Antibody

Detection of Human Pro-BDNF antibody by Western Blot.

Detection of Human Pro‑BDNF by Western Blot.

Western blot shows lysates of human brain (cerebellum) tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human Pro-BDNF Monoclonal Antibody (Catalog # MAB31751) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for Pro-BDNF at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of BDNF in Human Brain Cortex.

BDNF was detected in immersion fixed paraffin-embedded sections of human brain cortex using Mouse Anti-Human Pro BDNF Monoclonal Antibody (Catalog # MAB31751) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal processes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human Pro‑BDNF Antibody

Application
Recommended Usage

Immunohistochemistry

5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain cortex

Western Blot

2 µg/mL
Sample: Human brain (cerebellum) tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BDNF

BDNF is a member of the NGF family of neurotrophic factors that are required for the differentiation and survival of neuronal subpopulations in the central and peripheral nervous systems. BDNF functions through interactions with the TrkB receptor tyrosine kinase and the low affinity neurotrophin receptor, p75 (NTR). The human BDNF cDNA encodes 247 amino acids (aa). Cleavage of an 18 aa signal sequence produces an approximately 35 kDa Pro-BDNF form. The N-terminal pro region of BDNF is removed by tPA and furin to release biologically active, 14 kDa BDNF. The propeptides of human and mouse BDNF share 93% aa sequence identity.

Long Name

Brain-derived Neurotrophic Factor

Alternate Names

Abrineurin, ANON2, BULN2, Neurotrophin

Entrez Gene IDs

627 (Human); 12064 (Mouse); 24225 (Rat)

Gene Symbol

BDNF

UniProt

P23560

Additional BDNF Products

Product Documents for Human Pro‑BDNF Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Pro‑BDNF Antibody

For research use only

Citations for Human Pro‑BDNF Antibody

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Protocols

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