Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 289703
View all Meprin beta Subunit/MEP1B Primary Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell line HEK293-derived recombinant human Meprin beta Subunit/MEP1B
Thr23-Ser593
Accession # Q16820

Specificity

Detects human or mouse Pro Meprin beta Subunit/MEP1B in Western blots. It does not react with the mature form.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Applications for Human Pro Meprin  beta  Subunit/MEP1B Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human Meprin  beta  Subunit/MEP1B (Catalog # 2895-ZN)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Meprin beta Subunit/MEP1B

Meprins are multimeric proteases composed of alpha and beta  subunits, which are members of the astacin family of zinc endopeptidases (1, 2). Both subunits form disulfide‑linked homo- or heterooligomers, which are also referred to as Meprin A (composed of alpha  subunits with or without beta  subunits) and Meprin B (composed of beta  subunits only) (3). Although the two subunits share 42% identity in their amino acid sequence, they differ significantly in their oligomeric structure, post-translational processing and subsequently cellular location, and substrate and peptide bond specificity (4). The 701 aa sequence of the human Meprin beta subunit precursor consists of a signal peptide (aa 1 - 21), a pro region (aa 22 - 61), and a mature chain (aa 62 - 701) containing the following domains: catalytic (aa 62 - 259), MAM (aa 260 - 429), MATH (aa 430 - 585), EGF-like (aa 604 - 644), transmembrane (aa 653 - 673), and cytoplasmic (aa 674 - 701).

References

  1. Bond, J.S. and R.J. Beynon (1995) Protein Sci. 4:1247.
  2. Stocker, W. et al. (1995) Protein Sci. 4:823.
  3. Bertenshaw, G.P. et al. (2001) J. Biol. Chem. 276:13248.
  4. Ishmael, F.T. et al. (2005) J. Biol. Chem. 280:13895.

Alternate Names

MEP1B

Entrez Gene IDs

4225 (Human); 17288 (Mouse)

Gene Symbol

MEP1B

UniProt

Q16820

Additional Meprin beta Subunit/MEP1B Products

Product Documents for Human Pro Meprin  beta  Subunit/MEP1B Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Pro Meprin  beta  Subunit/MEP1B Antibody

For research use only

Related Research Areas

Metalloproteases and Regulators

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