Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Procalcitonin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Procalcitonin
Ala26-Gly117 (Asn57Asp)
Accession # P01258

Specificity

Detects human Procalcitonin in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Procalcitonin Antibody

Detection of Human Procalcitonin antibody by Western Blot.

Detection of Human Procalcitonin by Western Blot.

Western blot shows lysates of human thyroid cancer tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Procalcitonin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8350) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Procalcitonin at approximately 15-16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Procalcitonin Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human thyroid cancer tissue

Reviewed Applications

Read 1 review rated 5 using AF8350 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Procalcitonin

Human Procalcitonin (CALCA and Calcitonin) is 141 aa in length member of the calcitonin family. Precursor protein is cleaved into a propeptide and the following 2 chains: Calcitonin (aa 85-116) and Katacalcin (aa 121-141). Antigen used to develop this antibody is correspondent to the propeptide and Calcitonin part of the precursor molecule. Secreted Calcitonin is known to cause a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones. Over aa 26-117, human Procalcitonin shares 80% aa identity with mouse Procalcitonin. 

Alternate Names

CALC1, CALCA, Calcitonin 1, calcitonin gene-related peptide 1, CGRP, Katacalcin

Entrez Gene IDs

796 (Human); 12310 (Mouse); 24241 (Rat)

Gene Symbol

CALCA

UniProt

P01258

Additional Procalcitonin Products

Product Documents for Human Procalcitonin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Procalcitonin Antibody

For research use only

Customer Reviews for Human Procalcitonin Antibody (1)

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  • Human Procalcitonin Antibody
    Name: Balaji Mahender
    Application: ELISA
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 12/06/2017

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