Detection of Human Protein O‑Glucosyltransferase 1/POGLUT1 by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and IMR‑32 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Protein O‑Glucosyltransferase 1/POGLUT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6437) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Protein O‑Glucosyltransferase 1/POGLUT1 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Protein O-Glucosyltransferase 1/POGLUT1
O-Glucosyltransferase1 (POGLUT1) is a homologue of Rumi from Drosophila, an endoplasmic reticulum (ER)-retaining glucosyltransferase, that adds glucose to serine residues within the consensus sequence of C1‑X‑S‑X‑P‑C2 in Notch EGF repeats, thereby regulating cell-fate decisions (1). It is also known as CAP10-like protein (2) and KTELC1 due to the highly conserved CAP10 domain and the presence of an ER-retaining motif, KTEL, at the C‑terminus. The human gene is reported to be involved in the pathogenesis of both acute myelogenous and T‑acute lymphoblastic leukemias (3). Recently, POGLUT1 has been demonstrated in a phosphatase-coupled glycosyltransferase assay to have hydrolase activity on UDP-Glc (4).
Acar, M. et al. (2008) Cell 132:247.
Teng, Y. et al. (2006) Gene 371:7
Wang, Y. et al. (2010) Genet Test Mol Biomarkers 14:127.
Wu, Z.L. et al. (2010) Glycobiology, in press.
Entrez Gene IDs:
9630046K23Rik; C3orf9; CAP10-like protein, 46 kDa; chromosome 3 open reading frame 9; CLP46; EC 2.4.1.-; hCLP46CAP10-like 46 kDa protein; KDELC family like 1; KDELCL1; KTEL (Lys-Tyr-Glu-Leu) containing 1; KTEL motif-containing protein 1; KTELC1; MDS010; MDSRP; MDSRPKTELC1; MGC32995; Myelodysplastic syndromes relative protein; POGLUT1; protein O-glucosyltransferase 1; Rumi; x 010 protein
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