Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Protein O-Glucosyltransferase 1/POGLUT1 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Protein O-Glucosyltransferase 1/POGLUT1
Arg24-Leu388
Accession # Q8NBL1

Specificity

Detects human Protein O-Glucosyltransferase 1/POGLUT1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Protein O‑Glucosyltransferase 1/POGLUT1 Antibody

Detection of Human Protein O-Glucosyltransferase 1/POGLUT1 antibody by Western Blot.

Detection of Human Protein O‑Glucosyltransferase 1/POGLUT1 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and IMR-32 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human Protein O-Glucosyltransferase 1/POGLUT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6437) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Protein O-Glucosyltransferase 1/POGLUT1 at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Protein O‑Glucosyltransferase 1/POGLUT1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and IMR‑32 human neuroblastoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Protein O-Glucosyltransferase 1/POGLUT1

O-Glucosyltransferase1 (POGLUT1) is a homologue of Rumi from Drosophila, an endoplasmic reticulum (ER)-retaining glucosyltransferase, that adds glucose to serine residues within the consensus sequence of C1‑X‑S‑X‑P‑C2 in Notch EGF repeats, thereby regulating cell-fate decisions (1). It is also known as CAP10-like protein (2) and KTELC1 due to the highly conserved CAP10 domain and the presence of an ER-retaining motif, KTEL, at the C‑terminus. The human gene is reported to be involved in the pathogenesis of both acute myelogenous and T‑acute lymphoblastic leukemias (3). Recently, POGLUT1 has been demonstrated in a phosphatase-coupled glycosyltransferase assay to have hydrolase activity on UDP-Glc (4).

References

  1. Acar, M. et al. (2008) Cell 132:247.
  2. Teng, Y. et al. (2006) Gene 371:7
  3. Wang, Y. et al. (2010) Genet Test Mol Biomarkers 14:127.
  4. Wu, Z.L. et al. (2010) Glycobiology, in press.

Alternate Names

CLP46, KDELCL1, KTELC1, MDS010, MDSRP, POGLUT1, Rumi

Entrez Gene IDs

56983 (Human)

Gene Symbol

POGLUT1

UniProt

Q8NBL1

Additional Protein O-Glucosyltransferase 1/POGLUT1 Products

Product Documents for Human Protein O‑Glucosyltransferase 1/POGLUT1 Antibody

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Product Specific Notices for Human Protein O‑Glucosyltransferase 1/POGLUT1 Antibody

For research use only

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