Human PTF1A Antibody

Discontinued Product

AF6119 has been discontinued.
Detection of Human PTF1A by Western Blot.
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Human PTF1A Antibody Summary

Species Reactivity
Human
Specificity
Detects human PTF1A in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human PTF1A
Met1-Pro92
Accession # Q7RTS3
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
5-15 µg/mL
See below
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human PTF1A by Western Blot. View Larger

Detection of Human PTF1A by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human PTF1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6119) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PTF1A at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Immunocytochemistry PTF1A in Human Pancreatic Cancer Cells. View Larger

PTF1A in Human Pancreatic Cancer Cells. PTF1A was detected in immersion fixed human pancreatic cancer cells using Goat Anti-Human PTF1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6119) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry PTF1A in Human Pancreas. View Larger

PTF1A in Human Pancreas. PTF1A was detected in immersion fixed paraffin-embedded sections of human pancreas using Goat Anti-Human PTF1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6119) at 15 µg/mL overnight at
4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin‑embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PTF1A

PTF1A (Pancreas transcription factor 1a; also p48 and bHLHA29) is a cell specific member of the bHLH family of proteins. Although its predicted MW is 41 kDa, a high ratio of Pro, Leu and Ala increases its MW to 48 kDa in SDS-PAGE. PTF1A forms a trimeric transcription factor complex with a p75 subunit (HEB, E2 or E47) and a p64 subunit (usually RBP-L). Within this complex, unfortunately termed PTF1, p48/PTF1A binds to a DNA E-box while p64 binds to a TC-box. This regulates the gene transcription of about 20 enzymes synthesized by pancreatic acinar cells. Human PTF1A is 328 amino acids (aa) in length. It contains one basic DNA binding HLH motif (aa 150-175) and a dimerization interface (aa 178-219). Over aa 1-92, human PTF1A shares 88% aa identity with mouse PTF1A.

Long Name
Pancreas-specific Transcription Factor 1a
Entrez Gene IDs
256297 (Human); 19213 (Mouse); 117034 (Rat)
Alternate Names
bHLH transcription factor p48; BHLHA29; bHLHa29exocrine pancreas-specific transcription factor p48; Class A basic helix-loop-helix protein 29; p48 DNA-binding subunit of transcription factor PTF1; pancreas specific transcription factor, 1a; pancreas transcription factor 1 subunit alpha; Pancreas-specific transcription factor 1a; PTF1A; PTF1P48; PTF1-p48; PTF1-p48class II bHLH protein PTF1A

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