Human PTF1A Antibody
Human PTF1A Antibody Summary
Met1-Pro92
Accession # Q7RTS3
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human PTF1A by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human PTF1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6119) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PTF1A at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
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PTF1A in Human Pancreatic Cancer Cells. PTF1A was detected in immersion fixed human pancreatic cancer cells using Goat Anti-Human PTF1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6119) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red, upper panel; Catalog # NL001) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
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PTF1A in Human Pancreas. PTF1A was detected in immersion fixed paraffin-embedded sections of human pancreas using Goat Anti-Human PTF1A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6119) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PTF1A
PTF1A (Pancreas transcription factor 1a; also p48 and bHLHA29) is a cell specific member of the bHLH family of proteins. Although its predicted MW is 41 kDa, a high ratio of Pro, Leu and Ala increases its MW to 48 kDa in SDS-PAGE. PTF1A forms a trimeric transcription factor complex with a p75 subunit (HEB, E2 or E47) and a p64 subunit (usually RBP-L). Within this complex, unfortunately termed PTF1, p48/PTF1A binds to a DNA E-box while p64 binds to a TC-box. This regulates the gene transcription of about 20 enzymes synthesized by pancreatic acinar cells. Human PTF1A is 328 amino acids (aa) in length. It contains one basic DNA binding HLH motif (aa 150-175) and a dimerization interface (aa 178-219). Over aa 1-92, human PTF1A shares 88% aa identity with mouse PTF1A.
Product Datasheets
Citation for Human PTF1A Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Hematopoietic progenitors express embryonic stem cell and germ layer genes
Authors: Bernard Pessac, Mehdi Ait Bara, David Ford, Gopi K. Patibandla, David Trisler
Comptes Rendus Biologies
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot
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