Human Pygopus-2 Alexa Fluor® 750-conjugated Antibody

Catalog #: FAB3616S Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
FAB3616S-100UG

Save 15% on Select RUO Reagents. See Details.

All Pygopus-2 Antibodies

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Pygopus-2 Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Pygopus-2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Pygopus-1 or recombinant mouse Pygopus-1 is observed.

Source

Monoclonal Mouse IgG2b Clone # 539730

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human Pygopus-2
Met160-Glu336
Accession # NP_612157

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Pygopus-2

Human Pygopus-2 (pygopus; the name of a legless Australian lizard) is a 41 kDa, 406 amino acid (aa) transcriptional coactivator. It contains three domains: an N‑terminal NLS (aa 41-47), followed by a proline-rich region and a zinc-finger PHD-type domain (aa 330-387). A potential internal alternate start site may generate an isoform that deletes the first 37 aa. Pygopus 2 is a nuclear protein that acts in concert with BCL-9 and TCF to retain beta -Catenin in the nucleus during Wnt-signaling. It does so by forming a “chain-of-adaptors” with the above three proteins. Over the range of aa used for immunization, human pygopus 2 is 95% identical to mouse.

Entrez Gene IDs

90780 (Human); 68911 (Mouse)

Alternate Names

1190004M21Rik; FLJ33226; PYGO2; pygopus 2; pygopus homolog 2 (Drosophila); pygopus homolog 2; Pygopus2; Pygopus-2