Key Product Details

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG
View all Pygopus-2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Pygopus-2
Met160-Glu336
Accession # Q9BRQ0

Specificity

Detects human Pygopus-2 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human Pygopus-1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Pygopus‑2 Biotinylated Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human Pygopus‑2

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Pygopus-2

Pygopus-2 is a 41 kDa nuclear protein that functions as a transcriptional co-activator in the Wnt signaling pathway. Human Pygopus-2 is synthesized as a 406 amino acid (aa) residue protein that has the conserved N-terminal homology domain (NHD) and the C-terminal PHD (Plant homeodomain) zinc finger. The PHD zinc finger binds Legless/Bcl9, which in turn binds beta -Catenin, which recruits TCF/LEF DNA binding proteins. Formulation of the quaternary complex is required for the activation of Wnt-responsive genes. Over the region used for immunization, human Pygopus-2 shares 95% amino acid sequence identity with the mouse protein.

Alternate Names

PYGO2, Pygopus2

Entrez Gene IDs

90780 (Human); 68911 (Mouse)

Gene Symbol

PYGO2

UniProt

Q9BRQ0

Additional Pygopus-2 Products

Product Documents for Human Pygopus‑2 Biotinylated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Pygopus‑2 Biotinylated Antibody

For research use only

Citations for Human Pygopus‑2 Biotinylated Antibody

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