Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Pygopus-2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Pygopus-2
Met160-Glu336
Accession # Q9BRQ0

Specificity

Detects human Pygopus-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human Pygopus‑1 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Pygopus‑2 Antibody

Detection of Human Pygopus‑2 antibody by Western Blot.

Detection of Human Pygopus‑2 by Western Blot.

Western blot shows lysates of SH-SY5Y human neuroblastoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Pygopus-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3616) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Pygopus-2 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Pygopus-2 antibody in Human Ovary and Human Ovarian Cancer Tissue by Immunohistochemistry (IHC-P).

Pygopus‑2 in Human Ovary and Human Ovarian Cancer Tissue.

Pygopus-2 was detected in immersion fixed paraffin-embedded sections of normal human ovary (left panel) and ovarian cancer tissue (right panel) using Goat Anti-Human Pygopus-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3616) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Pygopus‑2 Antibody

Application
Recommended Usage

Immunohistochemistry

3-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections human ovary and human ovarian cancer tissue

Western Blot

1 µg/mL
Sample: SH‑SY5Y human neuroblastoma cell line and HeLa human cervical epithelial carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Pygopus-2

Pygopus-2 is a 41 kDa nuclear protein that functions as a transcriptional co-activator in the Wnt signaling pathway. Human Pygopus-2 is synthesized as a 406 amino acid (aa) residue protein that has the conserved N-terminal homology domain (NHD) and the C-terminal PHD (Plant homeodomain) zinc finger. The PHD zinc finger binds Legless/Bcl9, which in turn binds beta -Catenin, which recruits TCF/LEF DNA binding proteins. Formulation of the quaternary complex is required for the activation of Wnt-responsive genes. Over the region used for immunization, human Pygopus-2 shares 95% amino acid sequence identity with the mouse protein.

Alternate Names

PYGO2, Pygopus2

Entrez Gene IDs

90780 (Human); 68911 (Mouse)

Gene Symbol

PYGO2

UniProt

Q9BRQ0

Additional Pygopus-2 Products

Product Documents for Human Pygopus‑2 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Pygopus‑2 Antibody

For research use only

Citations for Human Pygopus‑2 Antibody

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Protocols

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