Detection of Human Pygopus‑2 by Western Blot. Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Pygopus‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3616) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Pygopus‑2 at approximately 53 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Pygopus‑2 in Human Ovary and Human Ovarian Cancer Tissue. Pygopus‑2 was detected in immersion fixed paraffin-embedded sections of normal human ovary (left panel) and ovarian cancer tissue (right panel) using Goat Anti-Human Pygopus‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3616) at 3 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Pygopus-2 is a 41 kDa nuclear protein that functions as a
transcriptional co-activator in the Wnt signaling pathway. Human
Pygopus-2 is synthesized as a 406 amino acid (aa) residue protein that
has the conserved N-terminal homology domain (NHD) and the C-terminal
PHD (Plant homeodomain) zinc finger. The PHD zinc finger binds
Legless/Bcl9, which in turn binds beta -Catenin, which recruits TCF/LEF DNA
binding proteins. Formulation of the quaternary complex is required for
the activation of Wnt-responsive genes. Over the region used for
immunization, human Pygopus-2 shares 95% amino acid sequence identity
with the mouse protein.
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