Human RAGE Antibody

Catalog # Availability Size / Price Qty
MAB11451-SP
MAB11451-100
MAB11451-500
Detection of Human RAGE by Western Blot.
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Product Details
Citations (15)
FAQs
Supplemental Products
Reviews (6)

Human RAGE Antibody Summary

Species Reactivity
Human
Specificity
Detects human RAGE in ELISAs and Western blots. In Western blots, approximately 25% cross-reactivity with recombinant mouse RAGE and recombinant rat RAGE is observed.
Source
Monoclonal Mouse IgG2B Clone # 176902
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human RAGE
Gln24-Ala344
Accession # Q15109
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.01 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Complete Your Experiment

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Blockade of Receptor-ligand Interaction
In a functional ELISA, 1-3 μg/mL of this antibody will block 50% of the binding of 500 ng/mL of biotinylated
AGE-BSA to immobilized recombinant human RAGE/Fc Chimera coated at 5 μg/mL (100 µL/well). At 10 μg/mL, this antibody will block >90% of the binding.
 

Human RAGE Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human RAGE Biotinylated Antibody (Catalog # BAF1145)

Standard: Recombinant Human RAGE Fc Chimera Protein, CF (Catalog # 1145-RG)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human RAGE by Western Blot. View Larger

Detection of Human RAGE by Western Blot. Western blot shows lysates of human lung tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human RAGE Monoclonal Antibody (Catalog # MAB11451) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for RAGE at approximately 43 kDa under non-reducing (NR) conditions and 50 kDa under reducing (R) conditions (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RAGE/AGER

Advanced glycation endproducts (AGE) are adducts formed by the non-enzymatic glycation or oxidation of macromolecules (1). AGE forms during aging and its formation is accelerated under pathophysiologic states such as diabetes, Alzheimer’s disease, renal failure and immune/inflammatory disorders. Receptor for Advanced Glycation Endoproducts (RAGE), named for its ability to bind AGE, is a multi-ligand receptor belonging the immunoglobulin (Ig) superfamily. Besides AGE, RAGE binds amyloid beta -peptide, S100/calgranulin family proteins, high mobility group B1 (HMGB1, also know as amphoterin) and leukocyte integrins (1, 2).

The human RAGE gene encodes a 404 amino acid residues (aa) type I transmembrane glycoprotein with a 22 aa signal peptide, a 320 aa extracellular domain containing an Ig-like V-type domain and two Ig-like Ce-type domains, a 21 aa transmembrane domain and a 41 aa cytoplasmic domain (3). The V-type domain and the cytoplasmic domain are important for ligand binding and for intracellular signaling, respectively. Two alternative splice variants, lacking the V-type domain or the cytoplasmic tail, are known (1, 4). RAGE is highly expressed in the embryonic central nervous system (5). In adult tissues, RAGE is expressed at low levels in multiple tissues including endothelial and smooth muscle cells, mononuclear phagocytes, pericytes, microglia, neurons, cardiac myocytes and hepatocytes (6). The expression of RAGE is upregulated upon ligand interaction. Depending on the cellular context and interacting ligand, RAGE activation can trigger differential signaling pathways that affect divergent pathways of gene expression (1, 7). RAGE activation modulates varied essential cellular responses (including inflammation, immunity, proliferation, cellular adhesion and migration) that contribute to cellular dysfunction associated with chronic diseases such as diabetes, cancer, amyloidoses and immune or inflammatory disorders (1).

References
  1. Schmidt, A. et al. (2001) J. Clin. Invest. 108:949.
  2. Chavakis, T. et al. (2003) J. Exp. Med. 198:507.
  3. Neeper, M. et al. (1992) J. Biol. Chem. 267:14998.
  4. Yonekura, H. et al. (2003) Biochem. J. 370:1097.
  5. Hori, O. et al. (1995) J. Biol. Chem. 270:25752.
  6. Brett, J. et al. (1993) Am. J. Pathol. 143:1699.
  7. Valencia, J.V. et al. (2004) Diabetes 53:743.
Long Name
Receptor for Advanced Glycation End Products
Entrez Gene IDs
177 (Human); 11596 (Mouse); 81722 (Rat); 403168 (Canine)
Alternate Names
advanced glycosylation end product-specific receptor; AGER; RAGE isoform delta; RAGE isoform sRAGE-delta; RAGE; Receptor for advanced glycosylation end products; receptor for advanced glycosylation end-products; SCARJ1

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Citations for Human RAGE Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

15 Citations: Showing 1 - 10
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  1. AGE/RAGE axis regulates reversible transition to quiescent states of ALK-rearranged NSCLC and pancreatic cancer cells in monolayer cultures
    Authors: T Kadonosono, K Miyamoto, S Sakai, Y Matsuo, S Kitajima, Q Wang, M Endo, M Niibori, T Kuchimaru, T Soga, K Hirota, S Kizaka-Kon
    Oncogene, 2022;12(1):9886.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Plasma sRAGE levels strongly associate with centrilobular emphysema assessed by HRCT scans
    Authors: F Klont, P Horvatovic, RP Bowler, E van Rikxoo, JP Charbonnie, M Kwiatkowsk, DA Lynch, S Humphries, R Bischoff, NHT Ten Hacken, SD Pouwels
    Respiratory Research, 2022;23(1):15.
    Species: Human
    Sample Types: Plasma
    Applications: Immunoprecipitation
  3. Platelets Fuel the Inflammasome Activation of Innate Immune Cells
    Authors: V Rolfes, LS Ribeiro, I Hawwari, L Böttcher, N Rosero, S Maasewerd, MLS Santos, T Próchnicki, CMS Silva, CWS Wanderley, M Rothe, SV Schmidt, HJ Stunden, D Bertheloot, MN Rivas, CJ Fontes, LH Carvalho, FQ Cunha, E Latz, M Arditi, BS Franklin
    Cell Rep, 2020;31(6):107615.
    Species: Human
    Sample Types:
    Applications: Inhibition
  4. Cerebrovascular amyloid Angiopathy in bioengineered vessels is reduced by high-density lipoprotein particles enriched in Apolipoprotein E
    Authors: J Robert, EB Button, EM Martin, L McAlary, Z Gidden, M Gilmour, G Boyce, TM Caffrey, A Agbay, A Clark, JM Silverman, NR Cashman, CL Wellington
    Mol Neurodegener, 2020;15(1):23.
    Species: Human
    Sample Types: Whole Tissue
    Applications: Neutralization
  5. Hydrophobicity drives receptor-mediated uptake of heat-processed proteins by THP-1 macrophages and dendritic cells, but not cytokine responses
    Authors: Y Deng, C Govers, M Teodorowic, I Liobyte, I De Simone, K Hettinga, HJ Wichers
    PLoS ONE, 2020;15(8):e0236212.
    Species: Human
    Sample Types: Protein
    Applications: ELISA Detection
  6. Transactivation of RAGE mediates angiotensin-induced inflammation and atherogenesis
    Authors: RJ Pickering, C Tikellis, CJ Rosado, D Tsorotes, A Dimitropou, M Smith, O Huet, RM Seeber, R Abhayaward, EK Johnstone, J Golledge, Y Wang, KA Jandeleit-, ME Cooper, KD Pfleger, MC Thomas
    J. Clin. Invest., 2019;129(1):406-421.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Polydatin protects against lipopolysaccharide-induced endothelial barrier disruption via SIRT3 activation
    Authors: J Wu, Z Deng, M Sun, W Zhang, Y Yang, Z Zeng, J Wu, Q Zhang, Y Liu, Z Chen, X Guo, KS Zhao, Q Huang, Z Chen
    Lab. Invest., 2019;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Claudin-18-mediated YAP activity regulates lung stem and progenitor cell homeostasis and tumorigenesis
    Authors: B Zhou, P Flodby, J Luo, DR Castillo, Y Liu, FX Yu, A McConnell, B Varghese, G Li, NO Chimge, M Sunohara, MN Koss, W Elatre, P Conti, JM Liebler, C Yang, CN Marconett, IA Laird-Offr, P Minoo, K Guan, BR Stripp, ED Crandall, Z Borok
    J. Clin. Invest., 2018;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  9. High-density lipoproteins suppress A?-induced PBMC adhesion to human endothelial cells in bioengineered vessels and in monoculture
    Authors: J Robert, EB Button, S Stukas, GK Boyce, E Gibbs, CM Cowan, M Gilmour, WH Cheng, SK Soo, B Yuen, A Bahrabadi, K Kang, I Kulic, G Francis, N Cashman, CL Wellington
    Mol Neurodegener, 2017;12(1):60.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  10. Autophagy-Lysosome Pathway in Renal Tubular Epithelial Cells Is Disrupted by Advanced Glycation End Products in Diabetic Nephropathy.
    Authors: Liu W, Shen T, Chen R, Wu H, Wang Y, Deng J, Chen Q, Pan Q, Huang Fu C, Tao J, Liang D, Liu H
    J Biol Chem, 2015;290(33):20499-510.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  11. Soluble receptor for advanced glycation end products as an indicator of pulmonary vascular injury after cardiac surgery.
    Authors: Tuinman P, Cornet A, Kuipers M, Vlaar A, Schultz M, Beishuizen A, Groeneveld A, Juffermans N
    BMC Pulm Med, 2013;13(0):76.
    Species: Human
    Sample Types: BALF
    Applications: ELISA Development
  12. Proteolytic release of the receptor for advanced glycation end products from in vitro and in situ alveolar epithelial cells.
    Authors: Yamakawa N, Uchida T, Matthay MA, Makita K
    Am. J. Physiol. Lung Cell Mol. Physiol., 2011;300(4):L516-25.
    Species: Rat
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  13. Effects of atorvastatin on serum soluble receptors for advanced glycation end-products in type 2 diabetes.
    Authors: Tam HL, Shiu SW, Wong Y, Chow WS, Betteridge DJ, Tan KC
    Atherosclerosis, 2010;209(1):173-7.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  14. Receptor for advanced glycation endproducts mediates neutrophil migration across intestinal epithelium.
    Authors: Zen K, Chen CX, Chen YT, Wilton R, Liu Y
    J. Immunol., 2007;178(4):2483-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot
  15. S100A11, an dual mediator for growth regulation of human keratinocytes.
    Authors: Sakaguchi M, Sonegawa H, Murata H, Kitazoe M, Futami J, Kataoka K, Yamada H, Huh NH
    Mol. Biol. Cell, 2007;19(1):78-85.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Reviews for Human RAGE Antibody

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Human RAGE Antibody
By Anonymous on 05/23/2019
Application: ELISA Sample Tested: Citrate Plasma Species: Human

Human RAGE Antibody
By Anonymous on 05/23/2019
Application: ELISA Sample Tested: Serum Species: Human

Human RAGE Antibody
By Anonymous on 05/23/2019
Application: ELISA Sample Tested: Citrate Plasma Species: Human

Human RAGE Antibody
By Anonymous on 08/07/2018
Application: ELISA Sample Tested: EDTA Plasma Species: Human

Human RAGE Antibody
By Anonymous on 08/07/2018
Application: ELISA Sample Tested: Serum Species: Human

Human RAGE Antibody
By Anonymous on 07/01/2018
Application: ICC/IF Sample Tested: HUMAN LUNG Species: Human