Detects human RAMP2 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse RAMP2 is observed and less than 1% cross-reactivity with recombinant human (rh) RAMP1 and rhRAMP3.
Polyclonal Sheep IgG
E. coli-derived recombinant human RAMP2 Gln43-Val145 Accession # O60895
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human RAMP2 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 0.5 µg/mL of Sheep Anti-Human RAMP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6427) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for RAMP2 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Human RAMP2 (receptor activity modifying protein 2) is a 17 kDa member of the RAMP family of proteins. It is expressed on cardiomyocytes, vascular smooth muscle cells and endothelium, and interacts with CRLR to form a receptor complex for adrenomedullin (AM). AM induces vasodilation on AM1 receptor expressing cells. Mature human RAMP2 is a 133 amino acid (aa) type I transmembrane glycoprotein that contains a 103 aa extracellular domain (ECD) (aa 43-145) and a nine aa cytoplasmic region. Although the ECD contains no typical structural motifs, aa 86-92 are critical for AM binding. There is one potential splice variant that shows a five aa insertion after Glu54. Over aa 43-145, human RAMP2 shares 61% aa identity with mouse RAMP2. Members of RAMP family of proteins are known to form complexes with apparent molecular weight of 25 kDa to 50 kDa that are resistant to denaturing and reducing agents.
Receptor Activity Modifying Protein 2
Entrez Gene IDs:
10266 (Human); 54409 (Mouse); 58966 (Rat)
calcitonin receptor-like receptor activity modifying protein 2; Calcitonin-receptor-like receptor activity-modifying protein 2; CRLR activity-modifying protein 2; RAMP2; receptor (calcitonin) activity modifying protein 2; receptor (G protein-coupled) activity modifying protein 2; receptor activity modifying protein 2; receptor activity-modifying protein 2; receptor-activity-modifying protein 2
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