Human RAMP2 Antibody

Catalog # Availability Size / Price Qty
AF6427
AF6427-SP
Human RAMP2 Antibody in Data
1 Image
Product Details
FAQs
Supplemental Products
Reviews

Human RAMP2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human RAMP2 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse RAMP2 is observed and less than 1% cross-reactivity with recombinant human (rh) RAMP1 and rhRAMP3.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human RAMP2
Gln43-Val145
Accession # O60895
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human RAMP2 antibody by Western Blot. View Larger

Detection of Human RAMP2 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 0.5 µg/mL of Sheep Anti-Human RAMP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6427) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for RAMP2 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RAMP2

Human RAMP2 (receptor activity modifying protein 2) is a 17 kDa member of the RAMP family of proteins. It is expressed on cardiomyocytes, vascular smooth muscle cells and endothelium, and interacts with CRLR to form a receptor complex for adrenomedullin (AM). AM induces vasodilation on AM1 receptor expressing cells. Mature human RAMP2 is a 133 amino acid (aa) type I transmembrane glycoprotein that contains a 103 aa extracellular domain (ECD) (aa 43-145) and a nine aa cytoplasmic region. Although the ECD contains no typical structural motifs, aa 86-92 are critical for AM binding. There is one potential splice variant that shows a five aa insertion after Glu54. Over aa 43-145, human RAMP2 shares 61% aa identity with mouse RAMP2. Members of RAMP family of proteins are known to form complexes with apparent molecular weight of 25 kDa to 50 kDa that are resistant to denaturing and reducing agents.

Long Name
Receptor Activity Modifying Protein 2
Entrez Gene IDs
10266 (Human); 54409 (Mouse); 58966 (Rat)
Alternate Names
calcitonin receptor-like receptor activity modifying protein 2; Calcitonin-receptor-like receptor activity-modifying protein 2; CRLR activity-modifying protein 2; RAMP2; receptor (calcitonin) activity modifying protein 2; receptor (G protein-coupled) activity modifying protein 2; receptor activity modifying protein 2; receptor activity-modifying protein 2; receptor-activity-modifying protein 2

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human RAMP2 Antibody

There are currently no reviews for this product. Be the first to review Human RAMP2 Antibody and earn rewards!

Have you used Human RAMP2 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review