|Detection of Human RAMP2 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 0.5 µg/mL of Sheep Anti-Human RAMP2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6427) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for RAMP2 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.|
Human RAMP2 (receptor activity modifying protein 2) is a 17 kDa member of the RAMP family of proteins. It is expressed on cardiomyocytes, vascular smooth muscle cells and endothelium, and interacts with CRLR to form a receptor complex for adrenomedullin (AM). AM induces vasodilation on AM1 receptor expressing cells. Mature human RAMP2 is a 133 amino acid (aa) type I transmembrane glycoprotein that contains a 103 aa extracellular domain (ECD) (aa 43-145) and a nine aa cytoplasmic region. Although the ECD contains no typical structural motifs, aa 86-92 are critical for AM binding. There is one potential splice variant that shows a five aa insertion after Glu54. Over aa 43-145, human RAMP2 shares 61% aa identity with mouse RAMP2. Members of RAMP family of proteins are known to form complexes with apparent molecular weight of 25 kDa to 50 kDa that are resistant to denaturing and reducing agents.
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