Human RanGAP1 Antibody

Catalog # Availability Size / Price Qty
Detection of Human RanGAP1 by Western Blot.
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Product Details
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Human RanGAP1 Antibody Summary

Species Reactivity
Detects human RanGAP1 in direct ELISAs and Western blots.
Polyclonal Sheep IgG
Antigen Affinity-purified
E. coli-derived recombinant human RanGAP1
Accession # P46060
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human RanGAP1 antibody by Western Blot. View Larger

Detection of Human RanGAP1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, NTera-2 human testicular embryonic carcinoma cell line, IMR-32 human neuroblastoma cell line, and HEK293T human embryonic kidney cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human RanGAP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7834) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for RanGAP1 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RanGAP1

RANGAP-1 (RAN [RAs-related Nuclear protein] GTPase-Activating Protein 1; also Fug1) is a 82-88 kDa member of the RNA1 family of molecules. It is widely expressed, and serves as a regulator of the nuclear regulatory protein RAN. RAN, when bound to GTP, is active, and is involved in the onset and termination of mitosis, plus the import and export of protein into the nucleus. In the cytosol, RAN:GTP is complexed to an Importin:cargo complex. RANGAP-1 converts the RAN‑associated GTP into GDP, and this induces dissociation of the complex into RAN:GDP, Importin and cargo. The cargo stays in the cytosol, while both RAN:GDP and Importin now enter the nucleus. Here, RAN:GDP is converted into RAN:GTP by RCC1, where it binds to a newly formed Importin:cargo complex, and is exported out of the nucleus, completing a cycle. While in the cytoplasm, RANGAP-1 is phosphorylated and stabilized through SUMOlyation and an interaction with Ubc9 and RanBP2. Human RANGAP-1 is 587 amino acids (aa) in length. It contains six LRRs (aa 48-343) plus two utilized acetylation sites and five phosphorylated Ser/Thr, and there is one SUMOylation motif between aa 523-526. There are three potential splice forms. One contains a single Ser insertion after Gly330, while a second contains an alternative start site 55 aa upstream of the standard site. A third isoform possesses that same upstream start site coupled to a 25 aa substitution for aa 525-587. Over aa 418-587, human RANGAP-1 shares 82% aa sequence identity with mouse RANGAP-1.

Long Name
Ran GTPase Activating Protein 1
Entrez Gene IDs
5905 (Human); 19387 (Mouse); 362965 (Rat)
Alternate Names
Fug1; KIAA1835Fug1; MGC20266; Ran GTPase activating protein 1; ran GTPase-activating protein 1; RanGAP1; SD; segregation distorter homolog (Drosophila); segregation distorter homolog; segregation distortion

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