Human RanGAP1 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human RanGAP1 in direct ELISAs and Western blots.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human RanGAP1
    Asn418-Val587
    Accession # P46060
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human RanGAP1 by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, NTera‑2 human testicular embryonic carcinoma cell line, IMR‑32 human neuroblastoma cell line, and HEK293T human embryonic kidney cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human RanGAP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7834) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for RanGAP1 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RanGAP1
RANGAP-1 (RAN [RAs-related Nuclear protein] GTPase-Activating Protein 1; also Fug1) is a 82-88 kDa member of the RNA1 family of molecules. It is widely expressed, and serves as a regulator of the nuclear regulatory protein RAN. RAN, when bound to GTP, is active, and is involved in the onset and termination of mitosis, plus the import and export of protein into the nucleus. In the cytosol, RAN:GTP is complexed to an Importin:cargo complex. RANGAP-1 converts the RAN‑associated GTP into GDP, and this induces dissociation of the complex into RAN:GDP, Importin and cargo. The cargo stays in the cytosol, while both RAN:GDP and Importin now enter the nucleus. Here, RAN:GDP is converted into RAN:GTP by RCC1, where it binds to a newly formed Importin:cargo complex, and is exported out of the nucleus, completing a cycle. While in the cytoplasm, RANGAP-1 is phosphorylated and stabilized through SUMOlyation and an interaction with Ubc9 and RanBP2. Human RANGAP-1 is 587 amino acids (aa) in length. It contains six LRRs (aa 48-343) plus two utilized acetylation sites and five phosphorylated Ser/Thr, and there is one SUMOylation motif between aa 523-526. There are three potential splice forms. One contains a single Ser insertion after Gly330, while a second contains an alternative start site 55 aa upstream of the standard site. A third isoform possesses that same upstream start site coupled to a 25 aa substitution for aa 525-587. Over aa 418-587, human RANGAP-1 shares 82% aa sequence identity with mouse RANGAP-1.
  • Long Name:
    Ran GTPase Activating Protein 1
  • Entrez Gene IDs:
    5905 (Human); 19387 (Mouse); 362965 (Rat)
  • Alternate Names:
    Fug1; KIAA1835Fug1; MGC20266; Ran GTPase activating protein 1; ran GTPase-activating protein 1; RanGAP1; SD; segregation distorter homolog (Drosophila); segregation distorter homolog; segregation distortion
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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