|RARRES3 in Raji Human Cell Line. RARRES3 was detected in immersion fixed Raji human Burkitt's lymphoma cell line using Human RARRES3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6139) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.|
RARRES3 (Retinoic acid receptor responder 3; also Tig3) is an 18-20 kDa member of the lecithin retinol acyltransferase family of molecules. It is widely expressed, particularly in keratinocytes, and is considered to have multiple functions. These include phospholipase A activity and an ability to both activate type I transglutaminase, and inhibit Ras activation Human RARRES3 is 164 amino acids (aa) in length. It contains a catalytic residue at Cys113, and a hydrophobic membrane-anchoring domain between aa 134-164. Within the cell, RARRES3 appears to be equally distributed between membrane and soluble fractions. RARRES3 is also reported to form higher order multimers. One potential isoform exists that shows a 26 aa substitution for aa 131-164. No meaningful structural ortholog of RARRES3 exists in rodent.
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