Human/Rat PIWIL2 Antibody Summary
Accession # Q8TC59
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Rat PIWIL2 by Western Blot. Western blot shows lysates of rat testis tissue. PVDF Membrane was probed with 1 µg/mL of Human PIWIL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6558) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PIWIL2 at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
PIWIL2 (PIWI [P-element-induced wimpy testis]-like 2; also HILI) is a 100-110 kDa cytosolic member of the PIWI subfamily, Argonaute family of proteins. It is expressed in the germ line, particularly in premeiotic and early meiotic germ cells, plus mesenchymal stem cells, and in select cancer stem cells such as CD44+CD24- breast cancer cells. PIWIL2 likely silences mobile genetic elements in germ cells, and appears to depress stem cell proliferation by inhibiting the expression of cell cycle genes. Human PIWIL2 is 973 amino acids (aa) in length. It contains one PAZ domain (aa 384-496) and a C-terminal PIWI domain (aa 668-959) that interact with RNA. There are multiple methylation sites on arginines. Two potential splice forms are reported, one that shows a deletion of aa 887-922, and a second that contains a 12 aa substitution for aa 486-801. Over aa 216-379, human PIWIL2 shares 96% aa identity with rat PIWIL2.
Citation for Human/Rat PIWIL2 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Differential L1 regulation in pluripotent stem cells of humans and apes.
Authors: Marchetto M, Narvaiza I, Denli A, Benner C, Lazzarini T, Nathanson J, Paquola A, Desai K, Herai R, Weitzman M, Yeo G, Muotri A, Gage F
Sample Types: Whole Cells
Applications: Western Blot
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