Human/Rat PIWIL2 Antibody

Catalog # Availability Size / Price Qty
Detection of Rat PIWIL2 by Western Blot.
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Product Details
Citations (1)
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Human/Rat PIWIL2 Antibody Summary

Species Reactivity
Human, Rat
Detects human PIWIL2 in direct ELISAs and human and rat PIWIL2 in Western blots. In direct ELISAs, less than 4% cross-reactivity with recombinant human PIWIL4 is observed.
Polyclonal Sheep IgG
Antigen Affinity-purified
E. coli-derived recombinant human PIWIL2
Accession # Q8TC59
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Rat PIWIL2 antibody by Western Blot. View Larger

Detection of Rat PIWIL2 by Western Blot. Western blot shows lysates of rat testis tissue. PVDF Membrane was probed with 1 µg/mL of Human PIWIL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6558) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PIWIL2 at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PIWIL2

PIWIL2 (PIWI [P-element-induced wimpy testis]-like 2; also HILI) is a 100-110 kDa cytosolic member of the PIWI subfamily, Argonaute family of proteins. It is expressed in the germ line, particularly in premeiotic and early meiotic germ cells, plus mesenchymal stem cells, and in select cancer stem cells such as CD44+CD24- breast cancer cells. PIWIL2 likely silences mobile genetic elements in germ cells, and appears to depress stem cell proliferation by inhibiting the expression of cell cycle genes. Human PIWIL2 is 973 amino acids (aa) in length. It contains one PAZ domain (aa 384-496) and a C-terminal PIWI domain (aa 668-959) that interact with RNA. There are multiple methylation sites on arginines. Two potential splice forms are reported, one that shows a deletion of aa 887-922, and a second that contains a 12 aa substitution for aa 486-801. Over aa 216-379, human PIWIL2 shares 96% aa identity with rat PIWIL2.

Long Name
Piwi-like Protein 2
Entrez Gene IDs
55124 (Human); 57746 (Mouse); 306011 (Rat)
Alternate Names
Cancer/testis antigen 80; CT80; CT80mili; FLJ10351; HILI; HILIMiwi like; MGC133049; MILI; PIWIL1L; PIWIL2; piwil2-like protein; piwi-like 2 (Drosophila); piwi-like protein 2

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Citation for Human/Rat PIWIL2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Differential L1 regulation in pluripotent stem cells of humans and apes.
    Authors: Marchetto M, Narvaiza I, Denli A, Benner C, Lazzarini T, Nathanson J, Paquola A, Desai K, Herai R, Weitzman M, Yeo G, Muotri A, Gage F
    Nature, 2013;503(7477):525-9.
    Species: Human
    Sample Types: Whole Cells
    Applications: Western Blot


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