PIWIL2 (PIWI [P-element-induced wimpy testis]-like 2; also HILI) is a 100-110 kDa cytosolic member of the PIWI subfamily, Argonaute family of proteins. It is expressed in the germ line, particularly in premeiotic and early meiotic germ cells, plus mesenchymal stem cells, and in select cancer stem cells such as CD44+CD24- breast cancer cells. PIWIL2 likely silences mobile genetic elements in germ cells, and appears to depress stem cell proliferation by inhibiting the expression of cell cycle genes. Human PIWIL2 is 973 amino acids (aa) in length. It contains one PAZ domain (aa 384-496) and a C-terminal PIWI domain (aa 668-959) that interact with RNA. There are multiple methylation sites on arginines. Two potential splice forms are reported, one that shows a deletion of aa 887-922, and a second that contains a 12 aa substitution for aa 486-801. Over aa 216-379, human PIWIL2 shares 96% aa identity with rat PIWIL2.
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Key Product Details
Species Reactivity
Validated:
Cited:
Applications
Validated:
Cited:
Label
Antibody Source
Product Specifications
Immunogen
Lys216-Asp379
Accession # Q8TC59
Specificity
Clonality
Host
Isotype
Scientific Data Images for PIWIL2 Antibody
Detection of Rat PIWIL2 by Western Blot.
Western blot shows lysates of rat testis tissue. PVDF Membrane was probed with 1 µg/mL of Human PIWIL2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6558) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PIWIL2 at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.Applications for PIWIL2 Antibody
Western Blot
Sample: Rat testis tissue
Formulation, Preparation, and Storage
Purification
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: PIWIL2
Long Name
Alternate Names
Gene Symbol
UniProt
Additional PIWIL2 Products
Product Documents for PIWIL2 Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for PIWIL2 Antibody
For research use only
Related Research Areas
Citations for PIWIL2 Antibody
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