Detects human Reg3A in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) Reg3G, recombinant mouse (rm) Reg3A, rmReg3B and recombinant rat Reg2 is observed and less than 2% cross-reactivity with rhReg1A, rhReg1B, rmRegIV, rmReg2 and rmReg3D.
Polyclonal Sheep IgG
E. coli-derived recombinant human Reg3A Glu27-Asp175 Accession # Q06141
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Detection of Human Reg3A by Western Blot.
Western blot shows lysates of human small intestine tissue. PVDF membrane was probed with 1 µg/mL of Human Reg3A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5940) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Reg3A at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Reg3A (regenerating gene III alpha ) is a secreted, 17 kDa, type III member of the Reg family, C-type lectin superfamily of proteins. It is expressed in intestine and exocrine pancreas, where it may have a protective effect against infection and TNF-alpha -induced stress. Mature mouse Reg3A is 149 amino acids (aa) in length (aa 27‑175). It is essentially a soluble C-type lectin domain (aa 40‑173). There are four type III genes ( alpha -δ) in the mouse Reg family. Reg3A has less that 60% aa identity to beta -δ. Mature mouse Reg3A is 87% and 61% aa identical to rat and human Reg3A, respectively.
Regenerating Islet-derived IIIa
Entrez Gene IDs:
5068 (Human); 19694 (Mouse); 171162 (Rat)
hepatocarcinoma-intestine-pancreas; HIPpancreatitis-associated protein; Human proislet peptide; Lithostathine 3; pancreatic beta cell growth factor; Pancreatitis-associated protein 1; PAP homologous protein; PAP1FLJ18565; PAP2; PAPPAP-H; PBCGF; proliferation-inducing protein 34; proliferation-inducing protein 42; Reg III-alpha; REG3; Reg3A; REG-3-alpha; regenerating islet-derived 3 alpha; regenerating islet-derived protein 3-alpha; Regenerating islet-derived protein III-alpha; REG-III
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