Human Resistin Quantikine ELISA Kit DRSN00: R&D Systems

Human Resistin Quantikine ELISA Kit

(16 citations)   
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (100 uL), Serum (20 uL), EDTA Plasma (20 uL), Heparin Plasma (20 uL)
  • Sensitivity
    0.055 ng/mL
  • Assay Range
    0.2 - 10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
  • Specificity
    Natural and recombinant human Resistin
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC237, Quantikine Immunoassay Control Group 237 - Please Inquire
Product Summary
The Quantikine Human Resistin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Resistin in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human Resistin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Resistin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Resistin.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample123123
n202020404040
Mean0.62.264.720.612.284.76
Standard Deviation0.030.120.180.050.210.37
CV%55.33.88.29.27.8

Recovery

The recovery of Resistin spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Tissue Culture Media (n=4) 99 96-103
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Resistin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Resistin Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Resistin
Resistin, also known ADSF and FIZZ3, is a secreted peptide hormone that circulates as either a noncovalent trimer, or a disulfide-linked homohexamer. Resistin is expressed by white adipocytes, splenocytes, astrocytes, and anterior pituitary epithelium. It blocks Insulin-stimulated uptake of glucose by adipocytes and promotes glucose release by hepatocytes. High circulating Insulin levels induce Resistin secretion which then antagonize Insulin action.
    • Entrez Gene IDs
      56729 (Human); 57264 (Mouse);
    • Alternate Names
      Adipose tissue-specific secretory factor; ADSF; ADSFMGC126609; C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich proteinprecursor 1; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine-rich secreted protein A12-alpha-like 2; Cysteine-rich secreted protein FIZZ3; FIZZ3; FIZZ3MGC126603; found in inflammatory zone 3; HXCP1; Resistin; RETN; RETN1; RSTNXCP1;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 100 µL Standard, Control, or Sample
    6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
    Citations:

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    16 Citations: Showing 1 - 10
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    Species
    Sample Type
    1. Circadian misalignment increases cardiovascular disease risk factors in humans
      Proc. Natl. Acad. Sci. U.S.A., 2016;113(10):E1402-11.
      Species: Human
      Sample Type: Serum
    2. Metabolic Syndrome and Selective Inflammatory Markers in Psoriatic Patients
      Authors: S Vachatova, C Andrys, J Krejsek, M Salavec, K Ettler, V Rehacek, E Cermakova, A Malkova, Z Fiala, L Borska
      J Immunol Res, 2016;2016(0):5380792.
      Species: Human
      Sample Type: Serum
    3. Serum levels of apoptosis inhibitor of macrophage are associated with hepatic fibrosis in patients with chronic hepatitis C.
      Authors: Mera K, Uto H, Mawatari S, Ido A, Yoshimine Y, Nosaki T, Oda K, Tabu K, Kumagai K, Tamai T, Moriuchi A, Oketani M, Shimada Y, Hidaka M, Eguchi S, Tsubouchi H
      BMC Gastroenterol, 2014;14(0):27.
      Species: Human
      Sample Type: Serum
    4. Effects of dietary fat modification on insulin sensitivity and on other risk factors of the metabolic syndrome-LIPGENE: a European randomized dietary intervention study.
      Authors: Tierney AC, McMonagle J, Shaw DI, Gulseth HL, Helal O, Saris WH, Paniagua JA, Golabek-Leszczynska I, Defoort C, Williams CM, Karsltrom B, Vessby B, Dembinska-Kiec A, Lopez-Miranda J, Blaak EE, Drevon CA, Gibney MJ, Lovegrove JA, Roche HM
      Int J Obes (Lond), 2011;35(6):800-9.
      Species: Human
      Sample Type: Serum
    5. Exenatide affects circulating cardiovascular risk biomarkers independently of changes in body composition.
      Authors: Bunck MC, Diamant M, Eliasson B, Corner A, Shaginian RM, Heine RJ, Taskinen MR, Yki-Jarvinen H, Smith U
      Diabetes Care, 2010;33(8):1734-7.
      Species: Human
      Sample Type: Serum
    6. Regulation of adipokine production in human adipose tissue by propionic acid.
      Authors: Al-Lahham SH, Roelofsen H, Priebe M, Weening D, Dijkstra M, Hoek A, Rezaee F, Venema K, Vonk RJ
      Eur. J. Clin. Invest., 2010;40(5):401-7.
      Species: Human
      Sample Type: Serum
    7. Improvements in coronary heart disease risk indicators by alternate-day fasting involve adipose tissue modulations.
      Authors: Bhutani S, Klempel MC, Berger RA, Varady KA
      Obesity (Silver Spring), 2010;18(11):2152-9.
      Species: Human
      Sample Type: Plasma
    8. Resistin, adiponectin, and risk of heart failure the Framingham offspring study.
      Authors: Frankel DS, Vasan RS, D'Agostino RB, Benjamin EJ, Levy D, Wang TJ, Meigs JB
      J. Am. Coll. Cardiol., 2009;53(9):754-62.
      Species: Human
      Sample Type: Plasma
    9. Decreased C-reactive protein-induced resistin production in human monocytes by simvastatin.
      Authors: Hu WL, Qian SB, Li JJ
      Cytokine, 2007;40(3):201-6.
      Species: Human
      Sample Type: Cell Culture Supernates
    10. Muscle-specific knockout of PKC-lambda impairs glucose transport and induces metabolic and diabetic syndromes.
      Authors: Farese RV, Sajan MP, Yang H, Li P, Mastorides S, Gower WR, Nimal S, Choi CS, Kim S, Shulman GI, Kahn CR, Braun U, Leitges M
      J. Clin. Invest., 2007;117(8):2289-301.
      Species: Human
      Sample Type: Serum
    11. Intake of phenol-rich virgin olive oil improves the postprandial prothrombotic profile in hypercholesterolemic patients.
      Authors: Ruano J, Lopez-Miranda J, de la Torre R, Delgado-Lista J, Fernandez J, Caballero J, Covas MI, Jimenez Y, Perez-Martinez P, Marin C, Fuentes F, Perez-Jimenez F
      Am. J. Clin. Nutr., 2007;86(2):341-6.
      Species: Human
      Sample Type: Plasma
    12. Obesity, adipokines, and abdominal aortic aneurysm: Health in Men study.
      Authors: Golledge J, Clancy P, Jamrozik K, Norman PE
      Circulation, 2007;116(20):2275-9.
      Species: Human
      Sample Type: Serum
    13. Resistin and GFR.
      Authors: Fagerberg B, Fagerlund C, Hulthe J
      Kidney Int., 2006;70(7):1371.
      Species: Human
      Sample Type: Serum
    14. Development of recombinant-based mass spectrometric immunoassay with application to resistin expression profiling.
      Authors: Tubbs KA, Kiernan UA, Niederkofler EE
      Anal. Chem., 2006;78(10):3271-6.
      Species: Human
      Sample Type: Plasma
    15. Relation of improvement in endothelium-dependent flow-mediated vasodilation after rosiglitazone to changes in asymmetric dimethylarginine, endothelin-1, and C-reactive protein in nondiabetic patients with the metabolic syndrome.
      Authors: Wang TD, Chen WJ, Cheng WC, Lin JW, Chen MF, Lee YT
      Am. J. Cardiol., 2006;98(8):1057-62.
      Species: Human
      Sample Type: Plasma
    16. Adiponectin and other adipocytokines as predictors of markers of triglyceride-rich lipoprotein metabolism.
      Authors: Chan DC, Watts GF, Ng TW, Uchida Y, Sakai N, Yamashita S, Barrett PH
      Clin. Chem., 2005;51(3):578-85.
      Species: Human
      Sample Type: Plasma
    Expand to show all 16 Citations
    ELISA Controls
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    Quantikine Immunoassay Control Group 237

    QC237
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    Supplemental ELISA Products
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    Quantikine ELISA Wash Buffer 1

    ELISA WA126 6
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