Human Resistin Quantikine ELISA Kit

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Human Resistin ELISA Standard Curve
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Product Details
Procedure
Citations (44)
FAQs
Supplemental Products
Reviews (1)

Human Resistin Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (20 uL), EDTA Plasma (20 uL), Heparin Plasma (20 uL)
Sensitivity
0.055 ng/mL
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Specificity
Natural and recombinant human Resistin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human Resistin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Resistin in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human Resistin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Resistin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Resistin.

Recovery

The recovery of Resistin spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Tissue Culture Media (n=4) 99 96-103

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Resistin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Resistin ELISA Linearity

Scientific Data

Human Resistin ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Resistin

Resistin, also known ADSF and FIZZ3, is a secreted peptide hormone that circulates as either a noncovalent trimer, or a disulfide-linked homohexamer. Resistin is expressed by white adipocytes, splenocytes, astrocytes, and anterior pituitary epithelium. It blocks Insulin-stimulated uptake of glucose by adipocytes and promotes glucose release by hepatocytes. High circulating Insulin levels induce Resistin secretion which then antagonize Insulin action.

Entrez Gene IDs:
56729 (Human); 57264 (Mouse)
Alternate Names:
Adipose tissue-specific secretory factor; ADSF; ADSFMGC126609; C/EBP-epsilon regulated myeloid-specific secreted cysteine-rich proteinprecursor 1; C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein; Cysteine-rich secreted protein A12-alpha-like 2; Cysteine-rich secreted protein FIZZ3; FIZZ3; FIZZ3MGC126603; found in inflammatory zone 3; HXCP1; Resistin; RETN; RETN1; RSTNXCP1
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 100 µL Standard, Control, or Sample
  6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human Resistin Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

44 Citations: Showing 1 - 10
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  1. Metabolic Syndrome and Adipokines Profile in Bipolar Depression
    Authors: Bilska, K;Dmitrzak-W?glarz, M;Osip, P;Pawlak, J;Paszy?ska, E;Permoda-Pachuta, A;
    Nutrients
    Species: Human
    Sample Types: Serum
  2. Adipokines and Inflammatory Markers in Acute Myocardial Infarction Patients with and without Obstructive Sleep Apnea: A Comparative Analysis
    Authors: Vega-Jasso, AL;Amezcua-Guerra, LM;González-Pacheco, H;Sandoval-Zárate, J;González-Díaz, CA;Escobar-Alvarado, J;Manzano-Luna, JD;Brianza-Padilla, M;
    International journal of molecular sciences
    Species: Human
    Sample Types: Serum
  3. Changes in Adipokine, Resitin, and BDNF Concentrations in Treatment-Resistant Depression after Electroconvulsive Therapy
    Authors: Permoda-Pachuta, A;Malewska-Kasprzak, M;Skibi?ska, M;Rzepski, K;Dmitrzak-W?glarz, M;
    Brain sciences
    Species: Human
    Sample Types: Serum
  4. A single nuclei atlas of aging human abdominal subcutaneous white adipose tissue
    Authors: Sparks, L;Whytock, K;Divoux, A;Sun, Y;Pino, M;Yu, G;Smith, S;Walsh, M;
    Research square
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Association of inflammatory biomarkers and disease activity with subclinical myocardial dysfunction in psoriatic arthritis
    Authors: Pletikosic, I;Marasovic Krstulovic, D;Bakovic, D;Susilovic Grabovac, Z;Tandara, L;Martinovic Kaliterna, D;
    Scientific reports
    Species: Human
    Sample Types: Serum
  6. Metformin Treatment of Hidradenitis Suppurativa: Effect on Metabolic Parameters, Inflammation, Cardiovascular Risk Biomarkers, and Immune Mediators
    Authors: R Hambly, N Kearney, R Hughes, JM Fletcher, B Kirby
    International Journal of Molecular Sciences, 2023-04-09;24(8):.
    Species: Human
    Sample Types: Serum
  7. Analysis of Selected Salivary Adipokines and Cytokines in Patients with Obesity-A Pilot Study
    Authors: L Ostrowska, J Smarkusz-Z, A Gornowicz, K Lendzion, B Zy?k, D Pogodzi?sk
    International Journal of Molecular Sciences, 2023-02-18;24(4):.
    Species: Human
    Sample Types: Saliva
  8. Pro-Inflammatory Adipokine and Cytokine Profiles in the Saliva of Obese Patients with Non-Alcoholic Fatty Liver Disease (NAFLD)-A Pilot Study
    Authors: B Zy?k, L Ostrowska, J Smarkusz-Z, K Witczak-Sa, A Gornowicz, A Bielawska
    International Journal of Molecular Sciences, 2023-02-02;24(3):.
    Species: Human
    Sample Types: Saliva
  9. Evaluation of the usefulness of determining the level of selected inflammatory biomarkers and resistin concentration in perivascular adipose tissue and plasma for predicting postoperative atrial fibrillation in patients who underwent myocardial revascularisation
    Authors: M Rachwalik, M Matusiewic, M Jasi?ski, M Hurkacz
    Lipids in Health and Disease, 2023-01-09;22(1):2.
    Species: Human
    Sample Types: Plasma
  10. Interleukin-19 Levels Are Increased in Palmoplantar Pustulosis and Reduced following Apremilast Treatment
    Authors: K Wolk, D Wilsmann-T, K Witte, TC Brembach, C Kromer, S Gerdes, K Ghoreschi, K Reich, R Mössner, R Sabat
    International Journal of Molecular Sciences, 2023-01-09;24(2):.
    Species: Human
    Sample Types: Plasma
  11. ADSCs stimulated by resistin promote breast cancer cell malignancy via CXCL5 in a breast cancer coculture model
    Authors: YY Wang, AC Hung, YC Wu, S Lo, HD Chen, YK Chen, YC Hsieh, SC Hu, MF Hou, SF Yuan
    Scientific Reports, 2022-09-14;12(1):15437.
    Species: Human
    Sample Types: Cell Culture Supernates
  12. Relationship between different serum cartilage biomarkers in the acute response to running and jumping in healthy male individuals
    Authors: M Dreiner, T Munk, F Zaucke, AM Liphardt, A Niehoff
    Scientific Reports, 2022-04-19;12(1):6434.
    Species: Human
    Sample Types: Serum
  13. Resistin production does not affect outcomes in a mouse model of acute surgical sepsis
    Authors: AS Bonavia, ZC Chroneos, V Ruiz-Velas, CH Lang
    PLoS ONE, 2022-03-14;17(3):e0265241.
    Species: Human
    Sample Types: Serum
  14. Changes in adipokine levels and metabolic profiles following bariatric surgery
    Authors: N Šebunova, J Štšepetova, T Kullisaar, K Suija, A Rätsep, I Junkin, H Soeorg, M Lember, T Sillakivi, R Mändar
    BMC endocrine disorders, 2022-02-03;22(1):33.
    Species: Human
    Sample Types: Serum
  15. Roles of plasma leptin and resistin in novel subgroups of type 2 diabetes driven by cluster analysis
    Authors: X Peng, J Huang, H Zou, B Peng, S Xia, K Dong, N Sun, J Tao, Y Yang
    Lipids in Health and Disease, 2022-01-07;21(1):7.
    Species: Human
    Sample Types: Plasma
  16. Exposure to Zearalenone Leads to Metabolic Disruption and Changes in Circulating Adipokines Concentrations in Pigs
    Authors: V Nagl, B Grenier, P Pinton, U Ruczizka, M Dippel, M Bünger, IP Oswald, L Soler
    Toxins, 2021-11-08;13(11):.
    Species: Porcine
    Sample Types: Serum
  17. Adiponectin as a novel biomarker of disease severity in alopecia areata
    Authors: A Stochmal, A Wa?kiel-Bu, S Chrostowsk, M Zaremba, A Rakowska, J Czuwara, L Rudnicka
    Scientific Reports, 2021-07-05;11(1):13809.
    Species: Human
    Sample Types: Serum
  18. AntiTNF-alpha therapy normalizes levels of lipids and adipokines in psoriatic patients in the real-life settings
    Authors: I Olejniczak, J Narbutt, J Ceryn, M Skibi?ska, I Bednarski, A Wo?niacka, J Sieniawska, M Kraska-Gac, M Ci??y?ska, J ?migielski, M Noweta, M Waszczykow, W Owczarek, A Reich, A Lesiak
    Scientific Reports, 2021-04-29;11(1):9289.
    Species: Human
    Sample Types: Serum
  19. Epigenetic Regulation of Processes Related to High Level of Fibroblast Growth Factor 21 in Obese Subjects
    Authors: T P?atek, A Polus, J Góralska, U Ra?ny, A Dziewo?ska, A Micek, A Dembi?ska-, B Solnica, M Malczewska
    Genes, 2021-02-21;12(2):.
    Species: Human
    Sample Types: Serum
  20. Adipocytokines in Untreated Newly Diagnosed Rheumatoid Arthritis: Association with Circulating Chemokines and Markers of Inflammation
    Authors: GK Vasileiadi, AC Lundell, Y Zhang, K Andersson, I Gjertsson, A Rudin, C Maglio
    Biomolecules, 2021-02-21;11(2):.
    Species: Human
    Sample Types: Plasma
  21. Plasma Adipokines in Patients Resuscitated from Cardiac Arrest: Difference of Visfatin between Survivors and Nonsurvivors
    Authors: YZ Chen, SQ Zhou, YQ Chen, H Peng, YG Zhuang
    Dis. Markers, 2020-01-14;2020(0):9608276.
    Species: Human
    Sample Types: Plasma
  22. The concentration of resistin in perivascular adipose tissue after CABG and postoperative atrial fibrillation
    Authors: M Rachwalik, M Obremska, D Zy?ko, M Matusiewic, K ?ciborski, M Jasi?ski
    BMC Cardiovasc Disord, 2019-12-16;19(1):294.
    Species: Human
    Sample Types: Tissue Homogenates
  23. Effects of Methylmercury and Theaflavin Digallate on Adipokines in Mature 3T3-L1 Adipocytes
    Authors: S Chauhan, K Dunlap, LK Duffy
    Int J Mol Sci, 2019-06-05;20(11):.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  24. Myokine/Adipokine Response to "Aerobic" Exercise: Is It Just a Matter of Exercise Load?
    Authors: Z He, Y Tian, PL Valenzuela, C Huang, J Zhao, P Hong, Z He, S Yin, A Lucia
    Front Physiol, 2019-05-29;10(0):691.
    Species: Human
    Sample Types: Serum
  25. Plasma leptin, but not resistin, TNF-? and adiponectin, is associated with echocardiographic parameters of cardiac remodeling in patients with coronary artery disease
    Authors: AD Farca?, A Rusu, MA Stoia, LA Vida-Simit
    Cytokine, 2018-01-08;103(0):46-49.
    Species: Human
    Sample Types: Plasma
  26. Key Clinical Factors Predicting Adipokine and Oxidative Stress Marker Concentrations among Normal, Overweight and Obese Pregnant Women Using Artificial Neural Networks
    Authors: M Solis-Pare, G Estrada-Gu, O Perichart-, A Montoya-Es, M Guzmán-Hue, H Borboa-Oli, E Bravo-Flor, A Cardona-Pé, V Zaga-Clave, E Garcia-Lat, G Gonzalez-P, JA Hernández-, C Irles
    Int J Mol Sci, 2017-12-28;19(1):.
    Species: Human
    Sample Types: Serum
  27. Is There Any Relationship between Plasma 25-Hydroxyvitamin D?, Adipokine Profiles and Excessive Body Weight in Type 2 Diabetic Patients?
    Authors: J Kocot, P Dziemidok, M Kie?czykow, J Kurzepa, G Szcze?niak, I Musik
    Int J Environ Res Public Health, 2017-12-23;15(1):.
    Species: Human
    Sample Types: Plasma
  28. Resistin as a Prooxidant Factor and Predictor of Endothelium Damage in Patients with Mild Acute Pancreatitis Exposed to Tobacco Smoke Xenobiotics
    Authors: M ?ciskalska, G Marek, Z Grzebienia, H Milnerowic
    Mediators Inflamm., 2017-09-26;2017(0):3039765.
    Species: Human
    Sample Types: Plasma
  29. Metabolic Syndrome and Selective Inflammatory Markers in Psoriatic Patients
    Authors: S Vachatova, C Andrys, J Krejsek, M Salavec, K Ettler, V Rehacek, E Cermakova, A Malkova, Z Fiala, L Borska
    J Immunol Res, 2016-12-21;2016(0):5380792.
    Species: Human
    Sample Types: Serum
  30. Circadian misalignment increases cardiovascular disease risk factors in humans
    Proc. Natl. Acad. Sci. U.S.A., 2016-02-08;113(10):E1402-11.
    Species: Human
    Sample Types: Serum
  31. Serum levels of apoptosis inhibitor of macrophage are associated with hepatic fibrosis in patients with chronic hepatitis C.
    Authors: Mera K, Uto H, Mawatari S, Ido A, Yoshimine Y, Nosaki T, Oda K, Tabu K, Kumagai K, Tamai T, Moriuchi A, Oketani M, Shimada Y, Hidaka M, Eguchi S, Tsubouchi H
    BMC Gastroenterol, 2014-02-13;14(0):27.
    Species: Human
    Sample Types: Serum
  32. Effects of dietary fat modification on insulin sensitivity and on other risk factors of the metabolic syndrome-LIPGENE: a European randomized dietary intervention study.
    Authors: Tierney AC, McMonagle J, Shaw DI, Gulseth HL, Helal O, Saris WH, Paniagua JA, Golabek-Leszczynska I, Defoort C, Williams CM, Karsltrom B, Vessby B, Dembinska-Kiec A, Lopez-Miranda J, Blaak EE, Drevon CA, Gibney MJ, Lovegrove JA, Roche HM
    Int J Obes (Lond), 2010-10-12;35(6):800-9.
    Species: Human
    Sample Types: Serum
  33. Exenatide affects circulating cardiovascular risk biomarkers independently of changes in body composition.
    Authors: Bunck MC, Diamant M, Eliasson B, Corner A, Shaginian RM, Heine RJ, Taskinen MR, Yki-Jarvinen H, Smith U
    Diabetes Care, 2010-04-27;33(8):1734-7.
    Species: Human
    Sample Types: Serum
  34. Regulation of adipokine production in human adipose tissue by propionic acid.
    Authors: Al-Lahham SH, Roelofsen H, Priebe M, Weening D, Dijkstra M, Hoek A, Rezaee F, Venema K, Vonk RJ
    Eur. J. Clin. Invest., 2010-03-25;40(5):401-7.
    Species: Human
    Sample Types: Serum
  35. Improvements in coronary heart disease risk indicators by alternate-day fasting involve adipose tissue modulations.
    Authors: Bhutani S, Klempel MC, Berger RA, Varady KA
    Obesity (Silver Spring), 2010-03-18;18(11):2152-9.
    Species: Human
    Sample Types: Plasma
  36. Resistin, adiponectin, and risk of heart failure the Framingham offspring study.
    Authors: Frankel DS, Vasan RS, D'Agostino RB, Benjamin EJ, Levy D, Wang TJ, Meigs JB
    J. Am. Coll. Cardiol., 2008-12-26;53(9):754-62.
    Species: Human
    Sample Types: Plasma
  37. Decreased C-reactive protein-induced resistin production in human monocytes by simvastatin.
    Authors: Hu WL, Qian SB, Li JJ
    Cytokine, 2007-11-19;40(3):201-6.
    Species: Human
    Sample Types: Cell Culture Supernates
  38. Obesity, adipokines, and abdominal aortic aneurysm: Health in Men study.
    Authors: Golledge J, Clancy P, Jamrozik K, Norman PE
    Circulation, 2007-10-29;116(20):2275-9.
    Species: Human
    Sample Types: Serum
  39. Muscle-specific knockout of PKC-lambda impairs glucose transport and induces metabolic and diabetic syndromes.
    Authors: Farese RV, Sajan MP, Yang H, Li P, Mastorides S, Gower WR, Nimal S, Choi CS, Kim S, Shulman GI, Kahn CR, Braun U, Leitges M
    J. Clin. Invest., 2007-08-01;117(8):2289-301.
    Species: Human
    Sample Types: Serum
  40. Intake of phenol-rich virgin olive oil improves the postprandial prothrombotic profile in hypercholesterolemic patients.
    Authors: Ruano J, Lopez-Miranda J, de la Torre R, Delgado-Lista J, Fernandez J, Caballero J, Covas MI, Jimenez Y, Perez-Martinez P, Marin C, Fuentes F, Perez-Jimenez F
    Am. J. Clin. Nutr., 2007-08-01;86(2):341-6.
    Species: Human
    Sample Types: Plasma
  41. Resistin and GFR.
    Authors: Fagerberg B, Fagerlund C, Hulthe J
    Kidney Int., 2006-10-01;70(7):1371.
    Species: Human
    Sample Types: Serum
  42. Relation of improvement in endothelium-dependent flow-mediated vasodilation after rosiglitazone to changes in asymmetric dimethylarginine, endothelin-1, and C-reactive protein in nondiabetic patients with the metabolic syndrome.
    Authors: Wang TD, Chen WJ, Cheng WC, Lin JW, Chen MF, Lee YT
    Am. J. Cardiol., 2006-08-28;98(8):1057-62.
    Species: Human
    Sample Types: Plasma
  43. Development of recombinant-based mass spectrometric immunoassay with application to resistin expression profiling.
    Authors: Tubbs KA, Kiernan UA, Niederkofler EE
    Anal. Chem., 2006-05-15;78(10):3271-6.
    Species: Human
    Sample Types: Plasma
  44. Adiponectin and other adipocytokines as predictors of markers of triglyceride-rich lipoprotein metabolism.
    Authors: Chan DC, Watts GF, Ng TW, Uchida Y, Sakai N, Yamashita S, Barrett PH
    Clin. Chem., 2005-01-13;51(3):578-85.
    Species: Human
    Sample Types: Plasma

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Human Resistin Quantikine ELISA Kit
By Anonymous on 06/17/2022
Sample Tested: Serum

Assay was simple to follow and yielded results within the standard range according to suggested serum dilution.