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Human RNF4 Alexa Fluor® 532-conjugated Antibody

Catalog #: AF7964X Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF7964X-100UG

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Human RNF4 Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human RNF4 in direct ELISAs and Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E.coli-derived recombinant human RNF4
Met1-Ile190
Accession # P78317
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Label
Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration
Sample
Western Blot
Optimal dilution of this antibody should be experimentally determined.
 
Immunohistochemistry
Optimal dilution of this antibody should be experimentally determined.
 
Immunocytochemistry
Optimal dilution of this antibody should be experimentally determined.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: RNF4

RNF4 (small nuclear ring finger protein, SNURF) is a RING-finger ubiquitin E3 ligase that ubiquitinates and mediates the proteasomal destruction of targets such as PML, PEA3, CENP1, and PARP1. In addition to the RING domain, RNF4 contains four SUMO-interacting motifs (SIMs) that function to recruit this ligase to poly-sumoylated substrates. RNF4 will autoubiquitinate in vitro, and will also ubiquitinate poly-SUMO chains.

Long Name
Ring Finger Protein 4
Entrez Gene IDs
6047 (Human)
Alternate Names
E3 ubiquitin ligase RNF4; EC 6.3.2.-; Protein SNURF; RES4-26; ring finger protein 4SNURFSLX5; RNF4; SLX5; Small nuclear ring finger protein; SNURF

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