Human RNF8 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human RNF8 in direct ELISAs and Western blots. In direct ELISAs, approximately 100% cross-reactivity with recombinant mouse RNF8 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human RNF8
    Met1-Val124
    Accession # O76064
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human RNF8 by Western Blot. Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and U2OS human osteosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human RNF8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7114) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for RNF8 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RNF8

RNF8 (RING [really interesting gene] finger protein 8; also E3 ubiquitin-protein ligase RNF8) is a 55-58 kDa member of the RNF8 family of proteins. It is ubiquitously expressed, and serves as an E3 ubiquitin ligase. RNF8 is recruited to DNA double-strand breaks by phosphoMDC1, where it promotes the ubiquitination of histones H2A and H2AX. This, in turn, may result in the recruitment of 53BP1, a scaffold protein that holds DNA damage response elements. Human RNF8 is 485 amino acids (aa) in length. It contains one forkhead associated domain (aa 38-92), a Gln-rich segment (aa 276-345), and one Zn-finger region (aa 403-441). There is one utilized phosphorylation site at Ser157. Two potential isoforms are reported. One shows an 18 aa substitution for aa 81-485, while another possesses a 36 aa substitution for aa 413-485. RNF8 apparently undergoes ubiquitination, generating multiple isoforms that run at 62-72 kDa in SDS-Page. Over aa 1-124, human RNF8 shares 74% aa identity with mouse RNF8.

  • Long Name:
    Ring Finger Protein 8
  • Entrez Gene IDs:
    9025 (Human); 58230 (Mouse)
  • Alternate Names:
    ActA Binding Protein 2; C3HC4-type zinc finger protein; EC 6.3.2; EC 6.3.2.-; FLJ12013; KIAA0646UBC13/UEV-interacting ring finger protein; ring finger protein (C3HC4 type) 8; ring finger protein 8E3 ubiquitin-protein ligase RNF8; RNF8
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Control

Ctrl 5-001-A 9
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Secondary Antibodies
Description Application Cat# Citations Images  

Sheep IgG HRP-conjugated Antibody

WB, Simple Western HAF016 14  
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Donkey Anti-Sheep IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL010 3
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Donkey Anti-Sheep IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL011 5
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Donkey Anti-Sheep IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL012 2
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Sheep IgG (H+L) PE-conjugated Antibody

Flow F0126  
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Donkey Anti-Sheep IgG Biotinylated Antibody

WB BAF016 2
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

Flow F0128
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